ipbbkz@gmail.com
+ 7 (727) 394 75 62
HomeАР 08855758 (superviser Kushnarenko S.)

АР 08855758 (superviser Kushnarenko S.)

Project brief description

(Project duration 27 months, 2020-2022)

 

  Project title: “Development of efficient micropropagation technology for commercially valuable walnut varieties for production of high-quality planting material for southeast Kazakhstan”.

Walnut is one of the most valuable nut-bearing crops in the world, the area of industrial plantings is about 50 million hectares, world production exceeds 1.5 million tons. Over the past five years, Kazakhstan has stepped up efforts to develop nut growing in the country. In 2017, the Kazakhstan Association of Producers and Processors of Nuts and Berries was registered. The area under industrial nut orchards is expanding annually in Almaty and Turkestan regions, where soil and climatic conditions are most suitable for walnut growing. World experience in the production of walnut shows that this is a highly profitable industry if high-quality, highly productive varieties are used. To obtain a uniform standard planting material while maintaining the positive features of the variety, it is necessary to propagate the material vegetatively, not by seeds. Traditionally, walnut varieties are propagated by budding, a very time-consuming and complicated procedure, while the most important issue remains to ensure the purity of stock and scion material, elimination of bacterial and fungal pathogens. It is almost impossible to propagate walnut by cuttings due to the extremely low percentage of cuttings rooting. Thus, there are still no efficient technologies for producing genetically homogeneous material throughout the year. In this regard, the development of technology for micropropagation of walnut is extremely relevant.

  Project goal: Development of efficient micropropagation technology for commercially valuable walnut varieties for the production of high-quality planting material adapted to southeast Kazakhstan.

  Expected results:

  1. Efficient disinfection protocol of plant material, aseptic collection of commercially valuable walnut varieties in vitro.
  2. Commercially rational propagation rate and rooting percentage of walnut varieties in vitro.
  3. Genetic passports of commercially valuable walnut varieties based on microsatellite DNA markers.
  4. A reliable protocol for shoot tip cryopreservation and cryogenic collection of commercially valuable walnut varieties.
  5. High-quality planting material for commercially valuable walnut varieties.

  Project Manager:

  Svetlana Kushnarenko, Head of the Germplasm Cryopreservation Laboratory at the Institute of Plant Biology and Biotechnology (IPBB). Candidate of Biological Sciences, Associate Professor. Over 170 scientific papers have been published, of which 18 are included in the Web of Science database, 12 in the Scopus database; h-index 8. Scopus ID 6507153914; Web of Science ID M-6616-2015.

  Research team members:

  Natalya Romadanova, Leading Researcher, Candidate of Biological Sciences. Over 80 scientific papers have been published; h-index 4. Scopus ID 36111616700.

  Dilyara Gritsenko, Head of the Laboratory of Molecular Biology at IPBB, PhD; h-index 2 (Web of Science), ORCID ID 0000-0001-6377-3711.

  Moldir Aralbaeva, Junior Researcher. Eighteen scientific works have been published, of which three publications are in the Web of Science and Scopus databases. H-index 1. Scopus ID 57195069563.

  Nazgul Rymkhanova, Junior Researcher.

  Natalya Mikhailenko, laboratory assistant.

 

  List of publications and patents of the Project executors for the period from 2015 to 2020.

  • Kushnarenko , Romadanova N., Aralbaeva M. In vitro conservation of the only endangered population of Corylus avellana L. in Kazakhstan // Research on Crops. – 2020. – Vol. 21, No. 4. – Р. 681–686.
  • Утегенова Г.А., Кушнаренко С.В., Қалыбаев Қ.Р., Шораұлы Б., Огарь Н.П. Оценка состояния дикорастущей популяции ореха грецкого (Juglans regia ) в Казахстане // Ізденістер, нәтижелер. Исследования, результаты. – 2019. – № 2. – С. 276-285.
  • Омашева М.Е., Махмутова И.А., Утегенова Г.А., Ромаданова Н.В., Кушнаренко С.В. Сравнительная оценка методов выделения геномной ДНК из свежих, засушенных и гербарных листьев Juglans regia и Corylus avellana // Ізденістер, нәтижелер. Исследования, результаты. – 2019. – № 2. – С. 225-232.
  • Кушнаренко С.В., Ромаданова Н.В., Огарь Н.П., Аралбаева М.М., Верзилов М.А. Современное состояние популяции лещины обыкновенной (Corylus avellana L.) в Казахстане // Вестник Карагандинского Университета. Серия «Биология. Медицина География» – 2019. – № 2 (94). – С. 99-104.
  • Патент на полезную модель № 4998 от 05.06.2020. Кушнаренко С.В., Аралбаева М.М., Ромаданова Н.В. «Способ размножения лещины обыкновенной». Регистрационный № 2019/0952.2. Дата поступления 30 октября 2019 г.
  • Ромаданова Н.В., Нурманов М.М., Махмутова И.А. Кушнаренко С.В. Производство суперэлитных саженцев сортов и клоновых подвоев яблони // Вестник науки Казахского агротехнического университета им. С. Сейфуллина (междисциплинарный). – 2018. – №3 (98). – С. 4-13.
  • Utegenova G., Pallister K., Kushnarenko S., Ozek G., Ozek T., Abidkulova K., Kirpotina L., Schepetkin I., Quinn M., Voyich J. Chemical composition and antibacterial activity of essential oils from Ferula species against methicillin-resistant Staphylococcus aureus // Molecules. – 2018. – 23(7). – Р. 1679-1696.
  • Kushnarenko S., Romadanova N., Aralbayeva, Zholamanova S., Alexandrova A., Karpova O. Combined ribavirin treatment and cryotherapy for efficient Potato virus M and Potato virus S eradication in potato (Solanum tuberosum L.) in vitro shoots // In vitro Cellular & Developmental Biology – Plant. – 2017. – V. 53 (4). – P. 425-432.
  • Romadanova N.V., Kushnarenko S.V., Karasholakova L.N. Development of a common PVS2 vitrification method for cryopreservation of several fruit and vegetable crops // In vitro Cellular & Developmental Biology – Plant. – 2017. – V. 53 (4). – P. 382-393.
  • Ромаданова Н.В., Махмутова И.А., Карашолакова Л.Н., Христенко А.А., Кушнаренко С.В. Оптимизация микроклонального размножения барабариса // Eurasian Journal of Applied Biotechnology. – 2017. – No.2. – P. 47-55.
  • Romadanova N.V., Mishustina S.A., Gritsenko D.A., Omasheva M.Y., Galiakparov N.N., Reed B.M., Kushnarenko S.V. Cryotherapy as a method for reducing the virus infection of apples (Malus) // CryoLetters. – 2016. – V. 37, No. 1. – P. 1-9.
  • Патент 001528 KZ, А01G 1/06. Способ получения оздоровленных саженцев яблони / Ромаданова Н.В., Кушнаренко С.В. // Министерство юстиции республики Казахстан. РГП на праве хоз. Вед. «Институт биологии и биотехнологии растений» КН МОН РК. – № 1528; заявл. 31.07.15/0244.2, опубл. 08.07.16. Бюл. № 8.

 

  Results obtained for 2020-2021

The work on the in vitro culture establishing and the formation of an aseptic collection of commercially valuable varieties and forms of walnuts has been completed. It was found that the use of a Plant Preservative Mixture (PPM) reduced bacterial and fungal contamination from an average of 41.7% to 22.1% and from 42.0% to 29.5%, respectively. The positive effect of PPM was noted on the percentage of formation of aseptic walnut plants, which averaged 16.7%. An aseptic collection of 10 commercially valuable varieties and forms of walnuts has been created.

Optimization of nutrient media for walnut micropropagation has been carried out. The values of the multiplication factor (Kp) ranged from 3.1 to 3.5, depending on the genotype. A two-stage method for rooting walnut shoots in in vitro culture has been developed, in which the percentage of shoots rooting ranged from 79.7 to 85.7%, depending on the genotype. The next step will be the transfer of walnut plants from in vitro culture to soil substrate.

Genotyping of 10 walnut accessions was carried out using SSR markers. For each walnut accession, DNA passports have been developed containing genetic profiles for 9 polymorphic markers (WGA1, WGA4, WGA9, WGA32, WGA69, WGA79, WGA118, WGA202, WGA276).

 

  Research publications

Publications in foreign editions

  1. a) publications in journals included in the Web of Science Core Collection database (Thomson Reuters, USA)

1 Kushnarenko S., Aralbayeva M., Rymkhanova N., Romadanova N. Plant Preservative Mixture increases the percentage of walnut aseptic shoots // In Vitro Cellular & Developmental Biology. – Animal. – 2021. – Vol. 57, supplement issue 1, June 2021. – P. S51.

Papers in editions of the Republic of Kazakhstan

  1. a) in journals, collections, incl. conference proceedings

1 Кушнаренко С.В. Создание криобанка гермоплазмы растений в Казахстане: 15 летний опыт работы // В сб. Сохранение и воспроизводство генетических ресурсов для устойчивого сельского хозяйства и обеспечения продовольственной безопасности в масштабах ОИС. Матер. конф. 25 мая 2021 г., Нур-Султан, 2021. – С. 67-70.

Abstracts

  1. a) in the proceedings of international conferences from near and far abroad:

1 Kushnarenko S., Romadanova N., Aralbayeva M., Rymkhanova N. Conserving Juglans regia L. from the natural population in Kazakhstan by cryopreservation of isolated embryonic axes and shoot tips // Conserving Exceptional Plants Symposium, Cincinnati Zoo & Botanical Garden, USA, October 19-21, 2021.

2 Кушнаренко С.В., Аралбаева М.М., Рымханова Н.Қ., Ромаданова Н.В., Турдиев Т.Т. Методы биотехнологии для сохранения генетических ресурсов грецкого ореха в Казахстане // Тез. докл. II Межд. науч. практ. конф. «Геномика и современные биотехнологии в размножении, селекциии сохранении растений», Ялта, 13-15 октября, 2021 г. – 2021. – С. 161.

3 Рымханова Н.Қ., Аралбаева М.М., Кушнаренко С.В. Создание асептической коллекции коммерчески ценных сортов грецкого ореха // Тез. докл. II Межд. науч. практ. конф. «Геномика и современные биотехнологии в размножении, селекции и сохранении растений», Ялта, 13-15 октября, 2021 г. – 2021. – С. 141.

  1. b) in the proceedings of international conferences of the Republic of Kazakhstan:

1 Аралбаева М., Ермекқызы Д., Джусупбекова А. Оптимизация методов микроклонального размножения и укоренения коммерчески-ценных сортов грецкого ореха (Juglans regia L.) // Матер. межд. науч. конф. студентов и молодых ученых «Фараби әлемі», Алматы, 6-8 апреля 2021 г. – 2021. – С. 11.

2 Джусупбекова А., Ермекқызы Д. Оптимизация метода криоконсервации изолированных зародышевых осей грецкого ореха Juglans regia L. // Матер. межд. науч. конф. студентов и молодых ученых «Фараби әлемі», Алматы, 6-8 апреля 2021 г. – 2021. – С. 34.