Laboratory of Clonal Propagation

General Information

The Laboratory of Clonal Propagation was created in 1th April 2008 by a decision of Academic Council of Institute. Laboratory located in a building at Prospect. Al-Farabi 93/8 and has 3 laboratory’s and light-culture’s rooms, greenhouse30 sq.m, experimental field 200 sq.m.There are5 qualified specialists, including Candidate of Biological Sciences, PhD-student and 2M.Sc.

Laboratory staff

HeadofLab.Valentina K. Mursaliyeva, PhD in Biology

Senior researcher Svetlana V. Nam

Junior researcherDarya N. Satybaldiyeva, PhD-student;

Junior researcherZhanara S.Kozhebayeva, M.Sc.

JuniorresearcherTlek M. Mukhanov, M.Sc.

Head of laboratory

Valentina K. Mursaliyeva, candidate of biological science, worked as head of the laboratory from 2008 years. She graduated from the Kazakh State University, the biological faculty. In 1999 she defended her PhD’s thesis on specialty «Plant Physiology and Biochemistry». A specialist in the field of plant tissue culture, plant physiology and biochemistry. Author 85 publications, including 50 articles, 4 guidelines, 5 patents.She was awarded an honorary diploma of the Ministry of Education and Science of the Republic of Kazakhstan

The main activities

  • plant cell, tissue and organ culture
  • highly efficient techniques for micropropagation of rare and economically valuable plant species
  • study of plants flora of Kazakhstan — promising sources of biology active substances(in collaboration with botanists and phytochemists)
  • study the biological activity of secondary metabolites in test-systems in vitro
  • development biotechnologies for Herbal biological active supplements

The major achievements

  • Completed a series of works on development of efficient technologies for micropropagation of ornamental, wood, medicinal and technical plants
  • the deposited collection in vitro commercially valuable rose cultivars is supported (+40C)
  • micropropagation technique and in vitro germplasm preservation for rare crocus species of Kazakhstan
  • the phytochemical analysis on the major classes of biologically active substances for the species Stachys siboldii, Сistanche salsa, Sanguisorba officinalis, Bergenia crassifolia, Crocus alatavicus, Allochrusa gypsophiloides and the evaluation of biological activity of phytoextracts in the system of plant bioassays and in vitro tissue culture
  • technology of production of extracts tannins Sanguisorba officinalis, Bergenia crassifolia and new biological preparations for the rhizogenesis induction of difficult rooting cuttings of fruit and ornamental crops
  • standards for dietary supplements based on Stachys siboldii with antidiabetic properties
  • protocols nutrient medium for callus and suspension cultures of some endemic species of flora of Central Kazakhstan
  • innovative patents of RK (7), standard of organization (3), guidance (4), catalog of rose and 45 articles


  • Development of biotechnology cloning of coniferous and apple plants containing endogenous cytokinins and evaluation their adaptive capacity in conditions in vitro, in situ and ex situ (2006-2008)
  • Development of technology for production of biologically active food additives from Stevia rebaudiana bertoni and Stachys sieboldii, grown in the conditions of Kazakhstan (with JSC «Institute of Industrial Biotechnology», Stepnogorsk 2006-2008)
  • Biotechnology of new dietary supplements and special dietary foods antidiabetic and restorative action (2009-2011)
  • Micropropagationand collection creation of roses for the production of elite planting material of commercially important varieties of domestic and foreign selection (2009-201)
  • The study of the biological activity of tannins and developing the scientific basis of their practical application (together with the laboratory of phys. And Biochemistry of Plants 2009-2011)
  • Development of biological products to increase the efficiency of reproduction of commercially valuable fruit and ornamental crops» (2012-2014)
  • Biotechnology of food additives based on Crocus — a natural source of food coloring and flavoring» (2012-2014);
  • Preparation the callus culture of endemic species of plants-producers of new natural compounds (2013-2014)
  • The development micropropagation technique and in vitro preservation of Crocus from flora of Kazakhstan (2013-2015)
  • The development of effective propagation technologies, germplasm preservation and restoration of degraded natural populations of endemic — Turkestan soap root (Allochrusa gypsophiloides Regel) for the rational utilization of its genetic resources in the industry (2015-2017)

Scientific cooperation with international and domestic organizations

Pamukkale University, Laboratory of secondary metabolites, Denizli, Turkey

University of Mugla Sıtkı Kochman, microbial biotechnology laboratory and the laboratory of cell cultures, Turkey

Ufa Scientific Center «Institute of Biology» the Russian Academy of Sciences

Institute of Botany of the National Academy of Sciences of Azerbaijan, Department of Experimental Botany

Central Botanical Garden of National Academy of Sciences of Belarus, Biochemistry and Plant Biotechnology Department

Main Botanical Garden of Russian Academy of Sciences, Laboratory of Plant Biotechnology

Russian State Agrarian University – MTAAnamed after K.A.Timiryazev, Department of Genetics, Biotechnology, Breeding and Seed

Institute of breeding and seed production of vegetable crops (VNIISSOK)RAAS,Laboratory ofintroduction and Seed

National Center of Biotechnology of Committee of Scienceof Ministry of Education and Science of the Republic of Kazakhstan (CS of MES  RK)

Institute of Botany and Phytointroduction CS MES RK, Laboratory of Plant Resources

Institute of Protection and Quarantine of CS MES RK, Laboratory of plant biotechnology

The Kazakh Academy of Nutrition (KAN)of the Academy of Medical Sciences of RK (AMS RK), Laboratory of specialized food products and biologically active additives to food

al-Farabi Kazakh National University, Department of Chemistry of Natural Compounds

LLP «Rosa KZ»

Issyk state arboretum (formerly JSC «Lesnoi pitomnik»)

Institute of Chemical Sciences named afterA.B. Bekturova

The International research and production holding “Phytochemistry”, Botany and Biotechnology Laboratory

Research-teaching activities

The undergraduate students, graduate and doctoral PhD of al-Farabi Kazakh National University,  Kazakh National Agrarian University and KKazakh State Women’s Teacher Training Uuniversity conducted biology and biotechnology research.In the laboratory 6 bachelors, 5 masters have been prepared, currently studying bachelor 2 and PhD-student

Foreign internships, honorary scientific knowledge, awards, scholarships

Pamukkale University (Turkey), Laboratory of secondary metabolites, methods for assessing the biological activities of plant extracts

GU «Ufa Scientific Center« Institute of Biology», ELISA assay

Regional Training Workshop «Using the technology of molecular markers in plant genetic resources diversity assessment» of the project «In Situ / On farm Conservation of Agricultural Biodiversity (Horticultural Crops and Wild Fruit Species) in Central Asia», UNEP-GEF

Mursaliyeva V.K. Diploma of the Ministry of Education and Science.

Nam S.V. Diploma of the Ministry of Education and Science.

Vecherko N.A.Grant holder of the State scientific grant of the President of the Republic of Kazakhstan for talented young scientists, 2009-2011

Satybaldiyeva D.N. I degree Diploma of winner in the Republican competition of student and scientific works in natural and technical sciences


Мурсалиева В.К., Сатыбалдиева Д.Н., Рахимбаев И.Р. Микроклональное размножение крокуса: методические рекомендации. Алматы: ИП Волкова, 2015, 24 с.

Мурсалиева В.К., Мухамбетжанов С.К., Нам С.В. Микроклональное размножение роз. Методическое руководство по организации и проведению работ. Алматы: ИП Волкова, 2011, 64 с.

Мурсалиева В.К., Мухамбетжанов С.К., Нам С.В. Каталог роз. Алматы: ИП Волкова, 2011, 52 с.

Есмагул К., Мурсалиева В.К. Руководство к практическим занятиям по биохимии. 2010, Алматы, 112 с.

Satybaldiyeva D., Mursaliyeva V., Rakhimbayev I., Zayadan B., Mammadov R. Preliminary Phytochemical Analysis and Antioxidant, Antibacterial Activities of Crocus alatavicus from Kazakhstan // Notulae Botanicae Horti Agrobotanici Cluj-Napoca (Romania), 2015, v.43(2), p.343-348.

Nam S.V., Mukhambetzhanov S.K., Mursaliyeva V.K., Rakhimbaev I. Micropropagation of rose cultivar ‘Pareo’// African J. of Biotechnology.  2011, v.10(46), pp. 9344-9346

Hiroshi Nakano, Kevin K. Schrader, Leonid K. Mamonov, Tatyana S. Kustova, Valentina K. Mursaliyeva, Charles L. Cantrell. Isolation and identification of flavobacterium columnare and streptococcus iniae antibacterial compounds from the terrestrial plant Atraphaxis laetevirens // J. of Agric. Food Chem.– 2012. – V. 60. – P. 10415-10419.

Satybaldiyeva D.N., Mursaliyeva V.K., Mammadov R., Zayadan B.K. Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L. // Inter. J. of Biology and Chemistry (KazNU), 2016, N.1

Satybaldiyeva D.N., Mammadov R., Mursaliyeva V.K., Zayadan B.K. Determination of reducing power and minimum inhibitory concentration of Crocus alatavicus extracts // Abstr. Book of Symposium on Euroasian Biodiversity, 2016, р. 348.

Mursaliyeva V.K., Satybaldiyeva D.N. Investigation of Crocus  alatavicus – endemic from flora of Kazakhstan // Abst. of 2nd Inter. Symp. Secondary Metabolites, 2014,  p. 54.

Kozhebayeva ZH.S., Mursalieva V.K. The influence of phytoextracts on rhizogenesis of severs apples in vitro // Int. conference “Using Genetic Biodiversity to Increase the Quality of Organically Grown Fruits”, Poland, 2013, p. 34.

Mursalieva V.K., Nam S.V. Influence of hydrolysable tannins on rhizogenesis rose in vitro // 1st International Symposium on Secondary Metabolitest Chemical, Biological and Biotechnological Properties. Turkey, 2011 г. P.132

МурсалиеваВ.К., НамС.В., КожебаеваЖ.С., ЖамбакинК.Ж., ШаушековЗ.К. ПолучениекаллуснойисуспензионнойкультурполыниказахскойArtemisia kasakorum (Krasch.) Pavl. // ВестникКазНУ. Серияэкологическая, 2015, №1/2 (43), с. 497-502.

ГемеджиеваН.Г., МурсалиеваВ.К., МухановТ.М. ОценкасовременногосостоянияприродныхпопуляцийAllochrusa gypsophiloides (Regel) Schischk. вЮжно-Казахстанскойобласти // ИзвестияНАНРК. Серия биологическая и медицинская, 2016, №1 (313), с. 22-29

Кожебаева Ж.С., Мурсалиева В.К. Оптимизация условий микроклонального размножения яблони Сиверса // Вестник КазНУ. Серия биологическая. 2014, №1/2 (60), с. 265-268.

Cатыбалдиева Д.Н., Нам С.В., Мурсалиева В.К., Заядан Б.К. Введение в культуру in vitro крокуса алатауского Crocus alatavicus L// Вестник КазНУ. Серия биологическая. 2014, №1/2 (60), с. 336-339.

Мурсалиева В.К., Сатыбалдиева Д.Н., Гемеджиева Н.Г., Султанова Н.А., Елибаева Н.С., Турашева С.К. Исследование состава и содержания биологически активных веществ бадана толстолистного (Bergenia crassifolia (L.) Fitsch.)  и кровохлебки лекарственной (Sanguisorba officinalis L.) // Вестник КазНУ. Серия биологическая. 2013, №1 (57), с. 82-87.

Ким Ю.В., Нам С.В., Мурсалиева В.К., Жамбакин К.Ж.  Оптимизация условий микроклонального размножения Clematis alpine сорта Rube // Исследования, результаты. 2011, №2, с. 14-19

Муравлева Л.Е., Кулмагамбетов И.Р., Синявский Ю.А., Сарсенбаев Б.А., Мурсалиева В.К и др. Влияние несимметричного диметилгидразина на уровень внеклеточных нуклеиновых кислот в крови растущих животных, получавших БАДы на основе стахиса и стевии. Фундаментальные исследования. Москва, 2009, № 6, с.30-34.

Мурсалиева В.К., Нам С.В., Шаденова Е.А.,Жумабеков Е.Ж. Особенности культивирования некоторых видов хвойных в условиях invitro // Биотехнология. Теория и практика. 2008, №.1., 28-35.

Стандарт организации: Биологически активные добавки к пище «Стахстевин»

Стандарт организации: Биологически активная добавка к пище «Настойка  стахиса»

Стандарт организации: Биологически активная добавка к пище «Стахисан»

Инновационный патент № 23840 Фиточай “Есентайский”. Мамонов Л.К., Пономарев Б.Н., Сарсенбаев Б.А., Мурсалиева В.К., Васильев Ю.И.

Инновационный патент №23839 Фиточай “Алматинский”.  Мамонов Л.К., Пономарев Б.Н., Мурсалиева В.К.

Инновационный патент № 24973. Способ повышения выхода биомассы при культивировании каллусов. Мамонов Л.К., Мурсалиева В.К., Пономарев Б.Н., Завадский В.А., Сатыбалдиева Д.Н.

Инновационный патент №26649. Способ стимулирования корнеобразования в культуре in vitro сельскохозяйственных растений и древесных культур. Мурсалиева В.К., Мамонов Л.К., Нам С.В., Пономарев Б.Н., Гемеджиева Н.Г.

Заключение о выдаче патента на полезную модель «Способ получения in vitro микроклубнелуковиц крокуса алатауского». Мурсалиева В.К., Сатыбалдиева Д.Н., Нам С.В., Рахимбаев И.Р.


Training practices, thesesimplementation for students of biology

Two-week training formicropropagationtechnique

Research — comprehensive assessment of growth-regulatory activity of new drugs / or compounds in various test systems (culture in vitro, bioassays, seeds, mikrocherenki, green cuttings, etc.)

For greenhouses, nurseries and floriculture farms

— Consultancy and methodological assistance in plant micropropagation;

— Development of technologies for clonal micropropagation of difficult propagatedand rare species with medicinal, edible and ornamental values;

— Replication of high-quality material on the customer’s order at any time of the year: production — plantlets in vitro, container culture, own-rooted seedlings

Лаборатория клонального размножения

Лаборатория клонального размножения

Лаборатория клонального размножения



Лаборатория клонального размножения