{"id":24543,"date":"2024-12-25T18:52:32","date_gmt":"2024-12-25T12:52:32","guid":{"rendered":"http:\/\/ipbb.kz\/eng\/?page_id=24543"},"modified":"2025-12-25T16:59:00","modified_gmt":"2025-12-25T10:59:00","slug":"ap-22787867-superviser-kumarbayeva-m","status":"publish","type":"page","link":"https:\/\/ipbb.kz\/eng\/ap-22787867-superviser-kumarbayeva-m\/","title":{"rendered":"AP 22787867 (superviser Kumarbayeva M.)"},"content":{"rendered":"

Brief description of the project <\/strong><\/p>\n

(2024-2026)<\/p>\n

\u00a0<\/strong>Project title<\/strong>:<\/strong> IRN AP22787867 \u00abMolecular screening and identification of isolates insensitive to strobulirin fungicides with Quinone Outside Inhibitor Qol in wheat\u00a0Pyrenophora tritici-repentis<\/em>\u00a0population\u00bb.<\/p>\n

\u00a0<\/strong>Relevance.\u00a0<\/strong> Tan spot of wheat, the causative agent of which is Pyrenophora tritict-repentis<\/em> (Ptr<\/em>), is the most aggressive among leaf diseases of wheat both worldwide and in Kazakhstan. Under favorable conditions for the development of the disease, crop losses reach 50-65%. The increased importance of tan spot of wheat in recent years is due to an increase in wheat acreage with a combination of direct sowing (no-till) and monoculture; the absence of wheat varieties resistant to diseases and the emergence of new Ptr races; using ineffective fungicides, which ensured the geographical spread of the pathogen over large areas. In this situation, the use of fungicides has become one of the most important tools to help reduce crop losses. Strobilurine fungicides with an external quinone inhibitor, Qol, are widely used against tan spot, but there are insufficient studies reporting the sensitivity of the most aggressive Ptr<\/em> isolates to fungicides. In Kazakhstan, studies aimed at studying the sensitivity of Ptr<\/em> isolates to fungicides have not been conducted before. The widespread use of strobilurin fungicides against leaf blotches requires a study of its mechanism, i.e. there is a need to understand the nature of resistance to strobulirin fungicides. In this regard, the molecular screening and identification of isolates of Qol insensitive to strobulirin fungicides in the wheat Pyrenophora tritici-repentis<\/em> population is an urgent problem.<\/p>\n

\u00a0<\/strong>The goal of the project<\/strong>: <\/strong>Molecular screening and identification of isolates insensitive to strobulirin fungicides with Quinone outside inhibitor Qol in wheat\u00a0Pyrenophora tritici-repentis<\/em>\u00a0population<\/p>\n

\u00a0<\/strong>Expected results<\/strong>:<\/p>\n

    \n
  1. Identification of the causative agent of tan spot -on wheat in Almaty, Kostanay and Akmola regions. Creation of a collection of monoconidial isolates of the causative agent of tan spot P. tritici-repentis<\/em>.<\/li>\n
  2. Molecular screening of P. tritici-repentis<\/em> monoconidial isolates for the presence of CHS-1 chitin synthase genes, ITS internal transcribed spacer, and ToxA<\/em> and ToxB <\/em>toxin formation genes.<\/li>\n
  3. To study the effects of fungicides with an external quinone inhibitor Qol on the resistance of wheat genotypes, depending on the presence of Tsn1<\/em> and Tsc2<\/em> tan spot resistance genes in them.<\/li>\n<\/ol>\n

    \u00a0<\/strong>Scientific Supervisor of the project<\/strong>:<\/strong> PhD, Senior Researcher at the IPBB, Kumarbayeva Madina.<\/p>\n

    \u00a0<\/strong>Research group<\/strong>:\u00a0 <\/strong><\/p>\n

      \n
    1. Keishilov Zh.S.-researcher<\/li>\n
    2. Aydana Kharipzhanova \u2013 PhD, Junior researcher<\/li>\n
    3. Bolatbekova A. A.-PhD doctoral student,<\/li>\n
    4. Zhanuzak D. Junior researcher<\/li>\n
    5. Bakhytuly K. Junior researcher<\/li>\n<\/ol>\n

      \u00a0<\/strong>List of publications of the project\u2019s participants (<\/strong>2018-2023) <\/strong><\/p>\n

        \n
      1. Kumarbayeva M.,<\/u> Kokhmetova\u00a0A.,\u00a0Kovalenko\u00a0N.,\u00a0Atishova M., Keishilov Zh., Aitymbetova K. Characterization of\u00a0Pyrenophora tritici-repentis<\/em>(tan spot of wheat) Races in Kazakhstan \/\/\u00a0Phytopathologia Mediterranea. \u2013\u00a0<\/em> \u2013 Vol. 6. \u2013 P. 243-257. IF \u2013 2.08. <\/em>WOS Q2. Scopus:\u00a0<\/strong>percentile 65.<\/strong><\/li>\n
      2. Kokhmetova A., Rathan N.D., Sehgal D., Malysheva A., Kumarbayeva M<\/u>., Atishova M., Bolatbekova A., Krishnappa G., Gultyaeva E., Kokhmetova A., Keishilov Zh., Bakhytuly K. QTL mapping for seedling and adult plant resistance to stripe and leaf rust in two winter wheat populations \/\/ Frontiers in Genetics. \u2013 2023. \u2013 Vol. 14. DOI: 10.3389\/fgene.2023.1265859. IF \u2013 3.7. WOS Q2. Scopus: percentile 53.<\/strong><\/li>\n
      3. Malysheva A., Kokhmetova A., Urazaliev R., Kumarbayeva M.,<\/u> Keishilov Z., Nurzhuma M., Bolatbekova A., Kokhmetova A. Phenotyping and Identification of Molecular Markers Associated with Leaf Rust Resistance in the Wheat Germplasm from Kazakhstan, CIMMYT and ICARDA \/\/ Plants. \u2013 2023. \u2013 Vol. 12(15). \u2013 P. 2786. DOI: 10.3390\/plants12152786. IF \u2013 4.5. WOS Scopus<\/strong>:<\/strong> percentile 83. <\/strong><\/li>\n
      4. Kokhmetova A.M, Rsaliyev S., Atishova M.M, Kumarbayeva M<\/u>.T, Malysheva A., Keishilov Z., Zhanuzak D., Bolatbekova A. Evaluation of wheat germplasm forresistanceto leaf rust (Puccinia triticina<\/em>) and identificationofthe sources of Lr<\/em> resistance genes using molecular markers \/\/ Plants. \u2013 2021. \u2013 Vol. 10(7). \u2013 P. 1484. DOI: 10.3390\/plants10071484. IF \u2013 3.935. WOS Scopus<\/strong>:<\/strong> percentile 56. <\/strong><\/li>\n
      5. Kokhmetova A., Kumarbayeva M<\/u>., Atishova M. Identification of high-yielding wheat genotypes resistant to Pyrenophora tritici-repentis<\/em> (tan spot) \/\/ Euphytica. \u2013 2021. \u2013 Vol. 217. \u2013 P. 97. DOI:10.1007\/s10681-021-02822-y IF 1.84.WOS Q2.<\/strong> Scopus<\/strong>: <\/strong>percentile 74.<\/strong><\/li>\n
      6. Kokhmetova A.M, Rsaliyev A., Malysheva A.A, Atishova M., Kumarbayeva M.T<\/u>, Keishilov Z. Identification of StripeRust Resistance Genes inCommon WheatCultivars and BreedingLines from Kazakhstan \/\/ Plants. \u2013 2021. \u2013 Vol. 10(11). \u2013 P. 2303. DOI: 3390\/plants10112303 IF \u20133.935. WOS Q1. Scopus<\/strong>: <\/strong>percentile 56.<\/strong><\/li>\n
      7. Kokhmetova A., Sehgal D., Ali S., Atishova M., Kumatbayeva M<\/u>., Leonova I., Dreisigacker S. Genome-wide association study of tan spot resistance in a hexaploid wheat collection from Kazakhstan \/\/ Frontiers in Genetics. \u2013 2021. \u2013 Vol. 11. \u2013 P. 581214. IF \u2013 4.7. WOS Q2. Scopus: percentile 53.<\/strong><\/li>\n
      8. Kumarbayeva M.T.<\/u>, Kovalenko N.M., Kremneva O.Yu., Atishova M.N., Keishilov Zh.S., Malysheva A.A., Zhanuzak D.K., Bolatbekova A.A., Kokhmetova A.M. Identification of Wheat Samples for Resistance to Toxins of Pyrenophora Tritici-Repentis<\/em> (Ptr<\/em>) \/\/ International Journal of Biology and Chemistry. \u2013 2022. \u2013 Vol. 15(1). \u2013 P. 64-72. DOI: 10.26577\/ijbch.2022.v15.i1.07. IF \u2013 0.3. WOS Q4. <\/strong><\/li>\n
      9. Malysheva A.A., Kokhmetova A.M., Kumarbayeva M.T<\/u>., Zhanuzak D.K., Bolatbekova A.A., Keishilov Zh.S., Gultyaeva E.I., Kokhmetova A.M., Tsygankov V., Dutbayev Y.B., Dubekova S.B. Identification of Carriers of Puccinia Striiformis Resistance Genes in the Population of Recombinant Inbred Wheat Lines \/\/ International Journal of Biology and Chemistry. \u2013 \u2013 Vol. 15(1). \u2013 P. 4-10. DOI: 10.26577\/ijbch.2022.v15.i1.01. IF \u2013 0.3. WOS Q4.<\/strong><\/li>\n
      10. Kokhmetova A., Kovalenko N.V., Kumarbayeva M<\/u>. Pyrenophora tritici-repentis<\/em> population structure in the Republic of Kazakhstan and identification of wheat germplasm resistant to tan spot \/\/ \u0412\u0430\u0432\u0438\u043b\u043e\u0432\u0441\u043a\u0438\u0439 \u0436\u0443\u0440\u043d\u0430\u043b \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0438 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438. \u2013 2020. \u2013 Vol. 24. \u2013 P. 722-729. IF \u2013 0.9. WOS <\/strong>Q<\/strong>3<\/strong>. Scopus<\/strong>: percentile <\/strong><\/li>\n
      11. 6Kokhmetova A., Atishova M., Kumarbayeva M<\/u>., Leonova I. Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot \/\/ \u0412\u0430\u0432\u0438\u043b\u043e\u0432\u0441\u043a\u0438\u0439 \u0436\u0443\u0440\u043d\u0430\u043b \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0438 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438. \u2013 2019. \u2013 Vol. 23(7). \u2013 P. 879-886. DOI:10.18699\/VJ19.562. IF \u2013 0.9. WOS Q4. Scopus: percentile <\/strong><\/li>\n
      12. \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0435\u0439\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u0492\u0430\u043b\u044b\u043c\u0431\u0435\u043a \u049a., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422. \u049a\u0430\u0437\u0430\u049b\u0441\u0442\u0430\u043d\u0434\u0430 \u04e9\u0441\u0456\u0440\u0456\u043b\u0435\u0442\u0456\u043d \u0431\u0438\u0434\u0430\u0439 \u0441\u043e\u0440\u0442\u0442\u0430\u0440\u044b\u043d\u044b\u04a3 \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437 Pyrenophora tritici-repentis \u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0442\u04e9\u0437\u0456\u043c\u0434\u0456\u043b\u0456\u0433\u0456\u043d\u0435 \u0444\u0438\u0442\u043e\u043f\u0430\u0442\u043e\u043b\u043e\u0433\u0438\u044f\u043b\u044b\u049b \u0441\u043a\u0440\u0438\u043d\u0438\u043d\u0433 \u0436\u04af\u0440\u0433\u0456\u0437\u0443 \/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2019. \u2013 \u21162. \u2013 \u0421.213-218.<\/li>\n
      13. \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u0492\u0430\u043b\u044b\u043c\u0431\u0435\u043a \u041a., \u041a\u0435\u0439\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u0420\u0441\u0430\u043b\u0438\u0435\u0432 \u0410.\u0421. \u0413\u0435\u043d\u0435\u0442\u0438\u043a\u043e-\u0441\u0435\u043b\u0435\u043a\u0446\u0438\u043e\u043d\u043d\u043e\u0435 \u0438 \u0444\u0438\u0442\u043e\u043f\u0430\u0442\u043e\u043b\u043e\u0433\u0438\u0447\u0435\u0441\u043a\u043e\u0435 \u0438\u0437\u0443\u0447\u0435\u043d\u0438\u0435 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u0438 \u043a \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437\u0443 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \u043f\u0448\u0435\u043d\u0438\u0446\u044b. \u0412\u0435\u0441\u0442\u043d\u0438\u043a \u041d\u0430\u0443\u043a\u0438 \u041a\u0430\u0437\u0430\u0445\u0441\u043a\u043e\u0433\u043e \u0410\u0433\u0440\u043e\u0442\u0435\u0445\u043d\u0438\u0447\u0435\u0441\u043a\u043e\u0433\u043e \u0423\u043d\u0438\u0432\u0435\u0440\u0441\u0438\u0442\u0435\u0442\u0430. \u21163(102) 2019. \u0421. 47-57.<\/li>\n
      14. \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430. \u0410.\u041c., \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432. \u0416.\u0421., \u0492\u0430\u043b\u044b\u043c\u0431\u0435\u043a. \u049a., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422. \u049a\u0430\u0437\u0430\u049b\u0441\u0442\u0430\u043d\u0434\u0430 \u04e9\u0441\u0456\u0440\u0456\u043b\u0435\u0442\u0456\u043d \u0431\u0438\u0434\u0430\u0439 \u0441\u043e\u0440\u0442\u0442\u0430\u0440\u044b\u043d\u044b\u04a3 \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437 Pyrenophora tritici-repentis \u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0442\u04e9\u0437\u0456\u043c\u0434\u0456\u043b\u0456\u0433\u0456\u043d\u0435 \u0444\u0438\u0442\u043e\u043f\u0430\u0442\u043e\u043b\u043e\u0433\u0438\u044f\u043b\u044b\u049b \u0441\u043a\u0440\u0438\u043d\u0438\u043d\u0433 \u0436\u04af\u0440\u0433\u0456\u0437\u0443 \/\/ \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u0259\u0442\u0438\u0436\u0435\u043b\u0435\u0440 \u2013 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b \u2013 2019. \u2116 2 (82). — \u0421.213-218.<\/li>\n
      15. \u041a\u0435\u0439\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041c\u0430\u0434\u0435\u043d\u043e\u0432\u0430 \u0410.\u041a., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u0416\u0438\u0433\u0438\u0442\u0431\u0435\u043a\u043e\u0432\u0430 \u0410.\u0414. \u041a\u04af\u0437\u0434\u0456\u043a \u0431\u0438\u0434\u0430\u0439 \u043a\u043e\u043b\u043b\u0435\u043a\u0446\u0438\u044f\u0441\u044b\u043d\u044b\u04a3 \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437\u0493\u0430 (Pyrenophora tritici-repentis) \u0442\u04e9\u0437\u0456\u043c\u0434\u0456\u043b\u0456\u0433\u0456\u043d \u0431\u0430\u0493\u0430\u043b\u0430\u0443 \/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2020. \u2013 \u21162. \u2013\u0411.128-135.<\/li>\n
      16. \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422. \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u0420\u0441\u0430\u043b\u0438\u0435\u0432 \u0410.\u0421. \u0424\u0438\u0442\u043e\u043f\u0430\u0442\u043e\u043b\u043e\u0433\u0438\u0447\u0435\u0441\u043a\u0438\u0439 \u0441\u043a\u0440\u0438\u043d\u0438\u043d\u0433 \u043d\u0430 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u044c \u043a \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437\u0443 \u0438 \u043e\u0446\u0435\u043d\u043a\u0430 \u0445\u043e\u0437\u044f\u0439\u0441\u0442\u0432\u0435\u043d\u043d\u043e-\u0446\u0435\u043d\u043d\u044b\u0445 \u043f\u0440\u0438\u0437\u043d\u0430\u043a\u043e\u0432 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \u043e\u0437\u0438\u043c\u043e\u0439 \u043c\u044f\u0433\u043a\u043e\u0439 \u043f\u0448\u0435\u043d\u0438\u0446\u044b \/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2020. \u2013 \u21162. \u2013\u0421.247-252.<\/li>\n
      17. Kumarbaeva M.T., Kokhmetova A.M., Keishilov Zh.S., Chudinov V., Zhanuzak D.K. 2022. Disease monitoring to determine the level of spread and development of the pathogen pyrenophora tritici-repentis in Kazakhstan \/\/ Herald of science of S. Seifullin KazATU. 1(112): 906. DOI:10.51452\/kazatu.2022.1(112).906<\/li>\n
      18. Keishilov Zh.S., Kokhmetova A.M., Kumarbaeva M.T., Bolatbekova A.A., Malysheva A.A., Kokhmetova A.M. 2022. Monitoring of leaf rust (puccinia recondita) of spring wheat in northern Kazakhstan 2019-2021 \/\/ Herald of science of S. Seifullin KazATU. 1(112): 930. DOI: 10.51452\/kazatu.2022.1(112).930<\/li>\n
      19. Kumarbayeva, M.K., A.M. Kokhmetova, N.M. Kovalenko, O.Yu. Kremneva, M.N. Atishova, Zh.S. Keishilov, A.A. Malysheva, D.K. Zhanuzak, A.A. Bolatbekova, and A.M. Kokhmetova. \u201cIdentification of Wheat Samples for Resistance to Toxins of Pyrenophora Tritici-Repentis (Ptr)\u201d. International Journal of Biology and Chemistry 15 (1):64-72. https:\/\/doi.org\/10.26577\/ijbch.2022.v15.i1.07<\/a>.<\/li>\n
      20. Malysheva, A.A., A.M. Kokhmetova, M.K. Kumarbayeva, D.K. Zhanuzak, A.A. Bolatbekova, Zh.S. Keishilov, E.I. Gultyaeva, A.M. Kokhmetova, V. Tsygankov, Y.B. Dutbayev, and S.B. Dubekova. 2022. \u201cIdentification of Carriers of Puccinia Striiformis Resistance Genes in the Population of Recombinant Inbred Wheat Lines\u201d. International Journal of Biology and Chemistry 15 (1):4-10. https:\/\/doi.org\/10.26577\/ijbch.2022.v15.i1.01<\/a>.<\/li>\n
      21. Kokhmetova, A., Malysheva, A., Kumarbayeva, M., Bolatbekova, A., & Kokhmetova, A. 2022. Evaluation of the wheat recombinant inbred lines for resistance to leaf rust. \u041d\u0430\u0443\u0447\u043d\u044b\u0439 \u0436\u0443\u0440\u043d\u0430\u043b \u00ab\u0414\u043e\u043a\u043b\u0430\u0434\u044b \u041d\u0410\u041d \u0420\u041a\u00bb, (2), 48\u201360. https:\/\/doi.org\/10.32014\/2022.2518-1483.147<\/a><\/li>\n
      22. \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432, \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430, \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430, \u041c.\u0422., \u0416\u0430\u043d\u0443\u0437\u0430\u049b, \u0414.\u041a., \u0420\u0441\u0430\u043b\u0438\u0435\u0432, \u0428.\u0421. \u0411\u0438\u0434\u0430\u0439\u0434\u044b\u04a3 \u0441\u0430\u0440\u044b \u0442\u0430\u0442 (Puccinia striiformis f.sp. tritici) \u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0410\u043b\u043c\u0430\u0442\u044b \u043e\u0431\u043b\u044b\u0441\u044b \u0431\u043e\u0439\u044b\u043d\u0448\u0430 2019\u20132021 \u0436\u044b\u043b\u0434\u0430\u0440\u044b \u0436\u04af\u0440\u0433\u0456\u0437\u0456\u043b\u0433\u0435\u043d \u043c\u043e\u043d\u0438\u0442\u043e\u0440\u0438\u043d\u0433\u0456 \/\/ \u0412\u0435\u0441\u0442\u043d\u0438\u043a \u041a\u0430\u0440\u0430\u0433\u0430\u043d\u0434\u0438\u043d\u0441\u043a\u043e\u0433\u043e \u0443\u043d\u0438\u0432\u0435\u0440\u0441\u0438\u0442\u0435\u0442\u0430. \u0421\u0435\u0440\u0438\u044f \u00ab\u0411\u0438\u043e\u043b\u043e\u0433\u0438\u044f. \u041c\u0435\u0434\u0438\u0446\u0438\u043d\u0430. \u0413\u0435\u043e\u0433\u0440\u0430\u0444\u0438\u044f\u00bb. \u2013 2022. \u2013 Vol. 2. \u2013 \u2116. 106. \u2013 P. 82-88. DOI31489\/2022BMG2\/82-88<\/li>\n
      23. \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041c\u0430\u043b\u044b\u0448\u0435\u0432\u0430 \u0410.\u0410., \u0411\u043e\u043b\u0430\u0442\u0431\u0435\u043a\u043e\u0432\u0430 \u0410.\u0410. \u0418\u0434\u0435\u043d\u0442\u0438\u0444\u0438\u043a\u0430\u0446\u0438\u044f \u0438\u0441\u0442\u043e\u0447\u043d\u0438\u043a\u043e\u0432 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u0438 \u043a \u0436\u0435\u043b\u0442\u043e\u0439 \u0440\u0436\u0430\u0432\u0447\u0438\u043d\u0435 (Puccinia striiformis westend f. sp. tritici) \u043f\u0448\u0435\u043d\u0438\u0446\u044b \u0432 \u043a\u043e\u043b\u043b\u0435\u043a\u0446\u0438\u0438 \u043e\u0437\u0438\u043c\u044b\u0445 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \/\/\u2013 \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440 \u2013 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 2023. \u2013 \u21162 (98). \u2013 \u0421. 89-101. DOI https:\/\/doi.org\/10.37884\/2-2023\/09<\/a><\/li>\n
      24. \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u041c\u0430\u043b\u044b\u0448\u0435\u0432\u0430 \u0410.\u0410., \u0411\u0430\u0445\u044b\u0442\u04b1\u043b\u044b \u049a. \u0411\u0438\u0434\u0430\u0439\u0434\u044b\u04a3 \u0441\u0435\u043f\u0442\u043e\u0440\u0438\u043e\u0437 (Septoria tritici) \u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0430\u043b\u043c\u0430\u0442\u044b \u043e\u0431\u043b\u044b\u0441\u044b \u0431\u043e\u0439\u044b\u043d\u0448\u0430 2022 \u0436\u044b\u043b\u044b \u0436\u04af\u0440\u0433\u0456\u0437\u0456\u043b\u0433\u0435\u043d \u043c\u043e\u043d\u0438\u0442\u043e\u0440\u0438\u043d\u0433\u0456 \/\/\u2013 \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440 \u2013 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 2023. \u2013 \u21162 (98). \u2013 \u0421. 225-235. DOI https:\/\/doi.org\/10.37884\/2-2023\/22<\/a><\/li>\n
      25. \u041c\u0430\u043b\u044b\u0448\u0435\u0432\u0430 \u0410.\u0410., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u041a\u0435\u0439\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u0414\u0443\u0431\u0435\u043a\u043e\u0432\u0430 \u0421. \u041c\u043e\u043b\u0435\u043a\u0443\u043b\u044f\u0440\u043d\u043e-\u0433\u0435\u043d\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0439 \u0441\u043a\u0440\u0438\u043d\u0438\u043d\u0433 Lr-\u0433\u0435\u043d\u043e\u0432 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u0438 \u0443 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \u043c\u044f\u0433\u043a\u043e\u0439 \u044f\u0440\u043e\u0432\u043e\u0439 \u043f\u0448\u0435\u043d\u0438\u0446\u044b \u0432 \u0443\u0441\u043b\u043e\u0432\u0438\u044f\u0445 \u044e\u0433\u043e\u0432\u043e\u0441\u0442\u043e\u043a\u0430 \u041a\u0430\u0437\u0430\u0445\u0441\u0442\u0430\u043d\u0430 \/\/ \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440 \u2013 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 2023. \u2013 \u21163 (99). \u2013 \u0421. 92-99. (\u041a\u041e\u041a\u0421\u041d\u0412\u041e).<\/li>\n
      26. \u0416\u0430\u043d\u0443\u0437\u0430\u043a \u0414.\u041a., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u0411\u043e\u043b\u0430\u0442\u0431\u0435\u043a\u043e\u0432\u0430 \u0410., \u0411\u0430\u0445\u044b\u0442\u0443\u043b\u044b \u041a., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c. \u0418\u0434\u0435\u043d\u0442\u0438\u0444\u0438\u043a\u0430\u0446\u0438\u044f \u043d\u043e\u0441\u0438\u0442\u0435\u043b\u0435\u0439 \u0433\u0435\u043d\u043e\u0432 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u0438 \u043a \u0436\u0435\u043b\u0442\u043e\u0439 \u0440\u0436\u0430\u0432\u0447\u0438\u043d\u0435 \u043d\u0430 \u043e\u0441\u043d\u043e\u0432\u0435 \u043c\u043e\u043b\u0435\u043a\u0443\u043b\u044f\u0440\u043d\u043e\u0433\u043e \u0441\u043a\u0440\u0438\u043d\u0438\u043d\u0433\u0430 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \u044f\u0440\u043e\u0432\u043e\u0439 \u043f\u0448\u0435\u043d\u0438\u0446\u044b \/\/ \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440 \u2013 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 2023. \u2013 \u21163 (99). \u2013 \u0421. 136-145. (\u041a\u041e\u041a\u0421\u041d\u0412\u041e).<\/li>\n
      27. \u041aumarbayeva \u041c.\u0422., \u041aokhmetova \u0410.\u041c., \u041calysheva A.A., Bolatbekova \u0410.\u0410., \u041aokhmetova \u0410.\u041c. Identification of sources of resistance to Septoria tritici in winter wheat germoplasm \/\/ \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 2023. \u2013 \u21164(100). \u2013 P. 57-62 (\u041a\u041e\u041a\u0421\u041d\u0412\u041e).<\/li>\n
      28. \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u0414\u0443\u0442\u0431\u0430\u0435\u0432 \u0415.\u0411., \u0425\u0430\u0440\u0456\u043f\u0436\u0430\u043d\u043e\u0432\u0430 \u0410.\u0406., \u0414\u0430\u0431\u0430\u0431\u0430\u0442 \u0410.\u0410. \u0416\u0430\u0437\u0434\u044b\u049b \u0436\u04b1\u043c\u0441\u0430\u049b \u0431\u0438\u0434\u0430\u0439 \u04af\u043b\u0433\u0456\u043b\u0435\u0440\u0456\u043d\u0456\u04a3 puccinia recondita \u0436\u04d9\u043d\u0435 bipolaris sorokiniana \u0430\u0443\u0440\u0443 \u049b\u043e\u0437\u0434\u044b\u0440\u0493\u044b\u0448\u0442\u0430\u0440\u044b\u043d\u0430 \u0442\u04e9\u0437\u0456\u043c\u0434\u0456\u043b\u0456\u0433\u0456\u043d \u0444\u0438\u0442\u043e\u043f\u043e\u0442\u043e\u043b\u043e\u0433\u0438\u044f\u043b\u044b\u049b \u0431\u0430\u0493\u0430\u043b\u0430\u0443 \/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2024. \u2116 2 \u2013 122-134 \u0431\u0435\u0442. (\u041a\u041a\u0421\u041e\u041d \u041c\u041e\u041d \u0420\u041a). DOI: https:\/\/doi.org\/10.37884\/2-2024\/12<\/a><\/li>\n
      29. \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422.,\u00a0\u0411\u043e\u043b\u0430\u0442\u0431\u0435\u043a\u043e\u0432\u0430 \u0410.\u0410.,\u041d\u04b1\u0440\u0436\u04b1\u043c\u0430\u00a0\u041c.\u041d.\u00a0\u049a\u0430\u0437\u0430\u049b\u0441\u0442\u0430\u043d\u043d\u044b\u04a3 \u0441\u043e\u043b\u0442\u04af\u0441\u0442\u0456\u043a \u0436\u04d9\u043d\u0435 \u043e\u04a3\u0442\u04af\u0441\u0442\u0456\u043a \u043e\u0431\u043b\u044b\u0441\u0442\u0430\u0440\u044b \u0431\u043e\u0439\u044b\u043d\u0448\u0430, \u0431\u0438\u0434\u0430\u0439\u0434\u044b\u04a3 \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437 (pyrenophora tritici-repentis)<\/em>\u00a0\u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0436\u04af\u0440\u0433\u0456\u0437\u0456\u043b\u0433\u0435\u043d \u043c\u043e\u043d\u0438\u0442\u043e\u0440\u0438\u043d\u0433\u0456\u00a0\/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2024.\u00a0\u2116 2\u00a0\u2013\u00a074-84\u00a0\u0431\u0435\u0442.\u00a0(\u041a\u041a\u0421\u041e\u041d \u041c\u041e\u041d \u0420\u041a).\u00a0DOI:\u00a0https:\/\/doi.org\/10.37884\/2-2024\/08 <\/a><\/li>\n
      30. \u0411\u0430\u0445\u044b\u0442\u0443\u043b\u044b \u041a., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u0423\u043c\u0438\u0440\u0437\u0430\u043a\u043e\u0432\u0430 \u0410.\u0422., \u041a\u0443\u043b\u0438\u0435\u0432 \u0410.\u0421., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422., \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421. (2024). \u041c\u043e\u043d\u0438\u0442\u043e\u0440\u0438\u043d\u0433\u0440\u0430\u0441\u043f\u0440\u043e\u0441\u0442\u0440\u0430\u043d\u0435\u043d\u0438\u044f Juglans regia L. \u0432 \u044e\u0436\u043d\u044b\u0445 \u0438 \u044e\u0433\u043e-\u0432\u043e\u0441\u0442\u043e\u0447\u043d\u044b\u0445 \u0440\u0435\u0433\u0438\u043e\u043d\u0430\u0445 \u041a\u0430\u0437\u0430\u0445\u0441\u0442\u0430\u043d\u0430 \u0438 \u043c\u043e\u0440\u0444\u043e\u043b\u043e\u0433\u0438\u0447\u0435\u0441\u043a\u043e\u0435 \u0438\u0437\u0443\u0447\u0435\u043d\u0438\u0435 \u043f\u043b\u043e\u0434\u043e\u0432 \u0433\u0440\u0435\u0446\u043a\u043e\u0433\u043e \u043e\u0440\u0435\u0445\u0430 \/\/ \u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440 \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b. \u2013 \u21164. \u2013 2024.<\/li>\n<\/ol>\n

        Results for 202<\/strong>4<\/strong>:<\/strong><\/p>\n

          \n
        1. Monitoring of the spread and development of tan spot in the main grain-growing regions of the country (in Almaty, Turkestan, Kostanay and Akmola regions) was carried out in order to identify the causative agent of tan spot. Infectious material was collected and monoconidial isolates of P. tritici-repentis<\/em> were isolated in pure culture. A collection of monoconidial isolates of Ptr was created, the racial composition was characterized and the virulence of the isolates was determined using Canadian differentiating varieties.<\/li>\n
        2. Phytopathological screening and phenological observations of a collection of wheat samples at the stage of an adult plant were carried out in the field conditions of the Almaty region. Phenological observations and evaluation of wheat lines for the main elements of productivity were carried out.<\/li>\n
        3. Isolation of monoconidial isolates of P. tritici-repentis<\/em> (Ptr) in pure culture and morphological identification of the isolates were carried out. As part of the study, the isolation of monoconidial isolates of Ptr into pure culture was carried out. A collection of 107 pure cultures of monoconidial isolates of P. tritici-repentis <\/em>was created for further studies, including pathogenicity tests and molecular characterization.<\/li>\n<\/ol>\n

          Publications (202<\/strong>4<\/strong>):<\/strong><\/p>\n

            \n
          1. \u041a\u0435\u0438\u0448\u0438\u043b\u043e\u0432 \u0416.\u0421., \u041a\u043e\u0445\u043c\u0435\u0442\u043e\u0432\u0430 \u0410.\u041c., \u041a\u0443\u043c\u0430\u0440\u0431\u0430\u0435\u0432\u0430 \u041c.\u0422.,\u00a0\u0411\u043e\u043b\u0430\u0442\u0431\u0435\u043a\u043e\u0432\u0430 \u0410.\u0410.,\u041d\u04b1\u0440\u0436\u04b1\u043c\u0430\u00a0\u041c.\u041d.\u00a0\u049a\u0430\u0437\u0430\u049b\u0441\u0442\u0430\u043d\u043d\u044b\u04a3 \u0441\u043e\u043b\u0442\u04af\u0441\u0442\u0456\u043a \u0436\u04d9\u043d\u0435 \u043e\u04a3\u0442\u04af\u0441\u0442\u0456\u043a \u043e\u0431\u043b\u044b\u0441\u0442\u0430\u0440\u044b \u0431\u043e\u0439\u044b\u043d\u0448\u0430, \u0431\u0438\u0434\u0430\u0439\u0434\u044b\u04a3 \u043f\u0438\u0440\u0435\u043d\u043e\u0444\u043e\u0440\u043e\u0437 (pyrenophora tritici-repentis)<\/em>\u00a0\u0430\u0443\u0440\u0443\u044b\u043d\u0430 \u0436\u04af\u0440\u0433\u0456\u0437\u0456\u043b\u0433\u0435\u043d \u043c\u043e\u043d\u0438\u0442\u043e\u0440\u0438\u043d\u0433\u0456\u00a0\/\/ \u00ab\u0406\u0437\u0434\u0435\u043d\u0456\u0441\u0442\u0435\u0440, \u043d\u04d9\u0442\u0438\u0436\u0435\u043b\u0435\u0440-\u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u044f, \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b\u00bb, \u2013\u0410\u043b\u043c\u0430\u0442\u044b, 2024.\u00a0\u2116 2\u00a0\u2013\u00a074-84\u00a0\u0431\u0435\u0442.\u00a0(\u041a\u041a\u0421\u041e\u041d \u041c\u041e\u041d \u0420\u041a).\u00a0DOI:\u00a0https:\/\/doi.org\/10.37884\/2-2024\/08 <\/a>\u041a\u041e\u041a\u041d\u0412\u041e<\/strong><\/li>\n
          2. Kumarbayeva \u041c, Kokhmetova A., Keishilov Zh. Identification of promising wheat lines resistant to tan spot (Pyrenophora tritici-repentis<\/em>) using breeding and molecular methods. ICPBB 2024, \u0420. 67. (Almaty, Kazakhstan). \u0442\u0435\u0437\u0438\u0441<\/strong><\/li>\n<\/ol>\n

             <\/p>\n

            Results for 2025<\/strong><\/p>\n

            Molecular screening of monoconidial isolates of Pyrenophora tritici-repentis<\/em> was performed to detect the presence of the chitin synthase gene CHS-1, the internal transcribed spacer (ITS), and the toxin-encoding genes ToxA and ToxB.<\/p>\n

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            1. As a result of the molecular screening, all examined P. tritici-repentis<\/em> isolates (100%) carried the housekeeping gene CHS-1, producing an amplicon of 275 bp, which confirmed the quality of the extracted DNA and the suitability of the isolates for PCR analysis. ITS sequence analysis revealed a fragment of approximately 700 bp in 76 isolates, showing 99% identity with P. tritici-repentis<\/em> and thus confirming their species identity; no amplification was observed with PTM primers, excluding P. teres f. maculata<\/em>.<\/li>\n<\/ol>\n

              Screening of toxin-encoding genes showed the presence of ToxA in 53 isolates (69.7%) and ToxB in 6 isolates (7.9%). Thus, the P. tritici-repentis<\/em> population is characterized by a high prevalence of the ToxA gene and a low frequency of ToxB, which refines the genetic structure and potential virulence of the studied isolates.<\/p>\n

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              1. Under laboratory conditions, the sensitivity of Pyrenophora tritici-repentis<\/em> isolates to QoI (strobilurin) fungicides (azoxystrobin and pyraclostrobin) and to the triazole fungicide propiconazole was evaluated. All isolates exhibited a dose-dependent reduction in mycelial growth with increasing fungicide concentrations.<\/li>\n<\/ol>\n

                For azoxystrobin, the mean inhibition of mycelial growth ranged from 23.0 to 48.4% across concentrations of 0.01\u2013100 \u00b5g\/mL. EC\u2085\u2080 values varied between 2.03 and 6.91 mg\/L, indicating reduced sensitivity and the presence of isolates with signs of resistance to QoI fungicides. Considerable inter-isolate variability was observed, ranging from highly sensitive isolates to those showing partial growth even at the highest concentrations.<\/p>\n

                Propiconazole showed substantially higher biological activity: mycelial growth inhibition reached 83.1%, and complete growth suppression was observed in most isolates at 10\u2013100 \u00b5g\/mL. EC\u2085\u2080 values ranged from 0.04 to 1.15 mg\/L, indicating high sensitivity of the pathogen population, although signs of partial resistance were detected in some isolates.<\/p>\n

                Overall, the results demonstrate that QoI fungicides are less effective against P. tritici-repentis<\/em> mycelial growth than propiconazole, and the observed variability in sensitivity highlights the need for resistance monitoring and fungicide rotation to ensure effective control of tan spot.<\/p>\n

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                1. During the study, Pyrenophora tritici-repentis isolates were propagated and inoculum was produced to establish an artificial infection background, followed by phytopathological screening of wheat under field and laboratory conditions. The infection background was created using colonized oat grains and naturally infected straw.<\/li>\n<\/ol>\n

                  Field evaluations conducted during the 2025 growing season showed that under pronounced atmospheric drought conditions, the expression of tan spot on the natural infection background was minimal, indicating age-related resistance in most of the tested wheat genotypes. The NDVI index ranged from 0.20 to 0.69, reflecting plant stress caused by moisture deficit and high temperatures. Phenological traits included 200\u2013212 days to heading, and plant height ranged from 40 to 95 cm.<\/p>\n

                  At the seedling stage, significant differences in virulence among Ptr isolates were observed. Isolates from the Almaty region (KZ-11-S-IF-KIZ-2024 and KZ-7-S-ZHAM-2024) exhibited moderate to high virulence, with disease scores ranging from 1.0 to 3.6, whereas the isolate from the Kostanay region (KZ-6-N-KOS-2024) showed low virulence (1.0\u20133.5, predominantly 1\u20132). A significant correlation was established between symptom severity and the genotypic characteristics of both the isolates and wheat cultivars (r = 0.63; p < 0.001).<\/p>\n

                  These results confirm the presence of regional differences in virulence of P. tritici-repentis and emphasize the importance of a comprehensive assessment of wheat resistance at different developmental stages, using isolates of diverse geographic origin.<\/p>\n

                  \u00a0<\/em><\/p>\n

                  Publications 2025<\/strong><\/p>\n

                  Articles in a peer-reviewed domestic publication with a non-zero impact factor (recommended by the SHEQAC MSHE RK)<\/em><\/strong><\/p>\n

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                  1. Keishilov Zh., Kokhmetova A., Kumarbayeva M., Nurzhuma M., Bakhytuly K. (2025). Monitoring of the distribution and development of tan spot (Pyrenophora tritici-repentis<\/em>) of spring wheat in the northern regions of Kazakhstan. Izdenister Natigeler, 2(106), 121\u2013131. https:\/\/doi.org\/10.37884\/2-2025\/12<\/a><\/li>\n
                  2. Kumarbayeva M.T., Kokhmetova A.M., Keishilov Zh.S., Bolatbekova A.A., Bakhytuly K, Kovalenko N.M. (2025). Identification of new sources of disease resistance in wheat. Izdenister Natigeler, 3 (107). https:\/\/doi.org\/10.37884\/3-2025\/30<\/li>\n
                  3. Keishilov Zh., Kokhmetova A., Kumarbayeva M., Bolatbekova A.A., Bakhytuly K. Assessment of resistance of winter wheat cultivars to tan spot (Pyrenophora tritici-repentis<\/em>) in the Almaty and Turkestan regions. Science and Education, Scientific and Practical Journal of Zhangir Khan West Kazakhstan Agrarian and Technical University, Vol. 3, Issue 3 (80), pp. 32\u201343, September 2025.DOI: https:\/\/doi.org\/10.52578\/2305-9397-2025-3-3-32-43<\/a><\/li>\n<\/ol>\n

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                    \"\"<\/a>\"\"<\/a>\"\"<\/a>\u00a0<\/em><\/strong><\/p>\n

                    Abstracts of the international conference <\/em><\/strong>(indicating the form of the report<\/em>)<\/strong><\/p>\n

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                    1. Kumarbayeva M. T., Kokhmetova A. M., Lapochkina I. F., Kovalenko N. M., Zeleneva Yu. V., Keishilov Zh., Bolatbekova A., Bakhytuly K., Kharipzhanova A. Identification of wheat germplasm resistant to tan spot (Pyrenophora tritici-repentis<\/em>) using genetic, breeding, and molecular approaches. In: Proceedings of the V International Scientific and Practical Conference \u201cCurrent Problems of Plant Immunity to Harmful Organisms\u201d. Saint Petersburg: All-Russian Institute of Plant Protection (VIZR), 2025, 35 p.<\/li>\n<\/ol>\n

                       <\/p>\n

                      \"\"<\/a>\"\"<\/a>\u00a0<\/em><\/p>\n

                      \u00a0<\/strong><\/p>\n

                       <\/p>\n","protected":false},"excerpt":{"rendered":"

                      Brief description of the project (2024-2026) \u00a0Project title: IRN AP22787867 \u00abMolecular screening and identification of isolates insensitive to strobulirin fungicides with Quinone Outside Inhibitor Qol in wheat\u00a0Pyrenophora tritici-repentis\u00a0population\u00bb. \u00a0Relevance.\u00a0 Tan spot of wheat, the causative agent of which is Pyrenophora tritict-repentis (Ptr), is the most aggressive among leaf diseases of wheat both worldwide and in Kazakhstan. Under favorable conditions for<\/p>\n

                      <\/div>\n

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