{"id":24559,"date":"2025-01-08T11:24:11","date_gmt":"2025-01-08T05:24:11","guid":{"rendered":"http:\/\/ipbb.kz\/eng\/?page_id=24559"},"modified":"2025-12-25T15:37:41","modified_gmt":"2025-12-25T09:37:41","slug":"ap23490860-supervisor-abugalieva-s-i","status":"publish","type":"page","link":"https:\/\/ipbb.kz\/eng\/ap23490860-supervisor-abugalieva-s-i\/","title":{"rendered":"AP23490860 (supervisor Abugalieva S.I.)"},"content":{"rendered":"

Brief description of the project <\/strong><\/p>\n

(2024-2026)<\/p>\n

\u00a0<\/strong><\/p>\n

Project title:<\/strong> IRN AP23490860 \u00abBiodiversity of wild species of the genus Allium<\/em> in southern and southeastern Kazakhstan\u00bb.<\/p>\n

\u00a0<\/strong><\/p>\n

Relevance.\u00a0<\/strong>Allium<\/em> L. is one of the largest genera in the tribe Allieae Dumort., and includes more than 1000 species. The primary center of biodiversity of the genus is Central Asia, including Kazakhstan. There are at least 127 species of the Allium genus in Kazakhstan, at least 29 of which are endemic. The Red Book of Wild Flora of Kazakhstan lists 12 species growing in the south, southeast, and east of the country. Onions have vast economic importance, are food and medicinal plants, and are widely used in medicine, industry, and horticulture. At the same time, due to the large size of the genus, the complex genetic structure caused by different ploidy and different numbers of main chromosomes, and the difficulty in determining morphological characters, there are difficulties in the taxonomic identification of species. Also, the genus is constantly replenished with newly discovered species. For example, in 2021, 5 new endemic onion species were described for the flora of Kazakhstan. Consequently, there is a need to clarify the taxonomy of the genus growing in Kazakhstan using modern molecular genetic and genomic methods, including complete sequencing of plastid genomes. The most informative tools in the study of genetic diversity and molecular taxonomy of plants are highly polymorphic microsatellite DNA markers (SSR-simple sequence repeats), universal DNA barcodes, and relatively inexpensive methods for determining complete sequences of chloroplast genomes. Plant-derived secondary metabolites play an important role in adaptation to various stress conditions. Studying the biochemical diversity of widespread species of the genus Allium<\/em> based on the analysis of secondary metabolites is important for scientific research.<\/p>\n

Studying the genetic diversity of wild species of the genus Allium<\/em> growing in southern and southeastern Kazakhstan is of great importance for the conservation of biological diversity, understanding the evolution and adaptation of plants to changing environmental conditions, and developing effective strategies for the management and conservation of natural resources.<\/p>\n

\u00a0<\/strong><\/p>\n

The goal of the project: <\/strong>To study the genetic diversity of wild species of the genus Allium<\/em> growing in the territory of southern and southeastern Kazakhstan.<\/p>\n

\u00a0<\/strong><\/p>\n

Expected results<\/strong>:<\/p>\n

    \n
  1. Plant populations of wild onion species will be collected and the collection of DNA collected in the territory of southern and south-eastern Kazakhstan will be replenished.<\/li>\n
  2. A quantitative and qualitative analysis of secondary metabolites of widespread onion species collected in southern and southeastern Kazakhstan will be carried out.<\/li>\n
  3. The population structure will be determined and the intra- and interspecific genetic diversity of Kazakhstan species of the genus Allium<\/em> will be studied.<\/li>\n
  4. The nucleotide sequences of DNA markers of the nuclear and chloroplast genome of Allium<\/em> species will be determined, and the phylogenetic tree of the genus will be reconstructed.<\/li>\n
  5. The complete nucleotide sequence of chloroplast genomes of certain species of the genus Allium<\/em> collected in southern and southeastern Kazakhstan will be determined.<\/li>\n
  6. The database \u201cBiodiversity of flora in Kazakhstan\u201d will be updated with information on the distribution and genetic diversity of species of the genus Allium<\/em>.<\/li>\n<\/ol>\n

     <\/p>\n

    Scientific Supervisor of the project:<\/strong> Saule Abugalieva, principal researcher, Doctor of Biological Sciences (genetics), Professor (biology). She has many years of experience in the field of molecular genetics. Co-author of more than 100 scientific articles were published, including 73 articles in peer-reviewed foreign scientific publications, indexed in Web of Science (H-index=12, https:\/\/www.webofscience.com\/wos\/author\/record\/450734<\/a>) and\/or Scopus (H-index=14, https:\/\/www.scopus.com\/authid\/detail.uri?authorId=6507029042&origin=recordpage<\/a>), more than 25 articles in journals recommended by the Committee for Quality Assurance in Science and Higher\u00a0 Education (CQASHE) of the Ministry of Science and Higher Education of the Republic of Kazakhstan . Co-author of 6 monographs, 5 scientific and methodological recommendations, 4 utility model patents. Co-author of 3 varieties of barley, 2 varieties of wheat, 1 variety of soybean. Awarded the badge \u201cFor merits in the development of science of the Republic of Kazakhstan\u201d (2017). Prof. Abugalieva was awarded by the governmental scientific scholarship for scientists and specialists who have made an outstanding contribution to the development of science and technology of the Republic of Kazakhstan (2018). She was awarded the Science Leader Diploma from Thomson Reuters and JSC NCTI (2015). She was a Best Researcher 2022 (Competition of the Ministry of Science and Higher Education of the Republic of Kazakhstan).<\/p>\n

     <\/p>\n

    Research group:\u00a0 <\/strong><\/p>\n

    Shyryn Almerekova<\/strong>, PhD (geobotany), associate professor, co-author of more than 40 scientific articles, including 25 articles in peer-reviewed publications indexed in Web of Science (https:\/\/www.webofscience.com\/wos\/author\/record\/2003633<\/a>) and Scopus (H-index \u2013 8, https:\/\/www.scopus.com\/authid\/detail.uri?authorId=57196952712<\/a>), of which 20 articles are related to the study of wild flora of Kazakhstan. She has successfully completed internships in scientific centers in Germany, Spain, Hungary, and South Korea. She was awarded a certificate of honor from the Ministry of Science and Higher Education (2022) and the state scientific scholarship for talented young scientists (2021).<\/p>\n

    Akerke Amalova<\/strong>, PhD (biology), researcher, has 7 years of work experience in the field of molecular genetics. The master’s specialty is geobotany. Co-author of 22 articles, 9 of them in the Scopus\/WoS database (H-index=3 in the Web of Science database (https:\/\/www.webofscience.com\/wos\/author\/record\/2003632<\/a>) and H-index=4 Scopus (https :\/\/www.scopus.com\/authid\/detail.uri?authorId=57213623570), trained in scientific centers in England and Lithuania.<\/p>\n

    Moldir Ye<\/strong>rmagambetova<\/strong>, PhD (geobotany), researcher, has 5 years of experience in botany and population genetics of wild plant species. Co-author of more than 20 scientific publications, including 14 articles indexed in Scopus H-index 5 (Scopus https:\/\/www.scopus.com\/authid\/detail.uri?authorId=57381827100<\/a>), and Web of Science. Trained in scientific centers of China, Hungary, England, and South Korea.<\/p>\n

    \u00a0<\/strong><\/p>\n

    List of publications of the project\u2019s participants (2018-2023) <\/strong><\/p>\n

      \n
    1. Yermagambetova M.,<\/u> Almerekova S.,<\/u> Turginov O., Sultangaziev O., Abugalieva S.,<\/strong> Turuspekov Y. Genetic Diversity and Population Structure of Juniperus seravschanica<\/em> collected in Central Asia \/\/ Plants (IF=4.8, Q1-Plant Sciences<\/em>; SJR=0.790, percentile: 83 \u2013 Plant Sciences<\/em>). \u2013 2023. \u2013 Vol. 12. \u2013 N. 16. \u2013 P. 2961. doi: 10.3390\/plants12162961<\/li>\n
    2. Yermagambetova M.<\/u>, Abugalieva S.,<\/strong> Turuspekov Y., Almerekova S.<\/u> Illumina sequencing data of the complete chloroplast genome of rare species Juniperus seravschanica<\/em> (Cupressaceae) from Kazakhstan \/\/ Data in Brief (IF=<\/em> 1, Q3- Multidisciplinary Sciences,<\/em> SJR=<\/em> 0.258, percentile: 72 \u2013 Multidisciplinary<\/em>). \u2013 2023. \u2013 Vol. 46. \u2013 P. 108866.<\/li>\n
    3. Abugalieva S.,<\/strong> Volkova L., Genievskaya Y., Ivaschenko A., Kotukhov Y., Sakauova G., Turuspekov Y. Taxonomic assessment of Allium<\/em> species from Kazakhstan based on ITS and mat<\/em>K markers \/\/ BMC Plant Biology<\/em><\/strong> (IF=5.9, Q1 \u2013 Plant sciences; SJR=1.159, percentile: 92-Plant Science<\/em>). \u2013 2017. \u2013 Vol.17 (2). \u2013 \u0420.51-60. DOI: 10.1186\/s12870-017-1194-0<\/li>\n
    4. Almerekova S., Yermagambetova M.,<\/u> Abugalieva S.,<\/strong> Turuspekov Y. The complete chloroplast genome sequencing data of Juniperus sabina<\/em> (Cupressaceae Bartl.) from Kazakhstan.\u00a0Data in Brief<\/em><\/strong> (IF<\/em>= 1.1, Q3 Multidisciplinary sciences; SJR= 0.131, percentile: 72- Multidisciplinary)<\/em>. \u2013 2022. \u2013 Vol.45. \u2013 P.108644. DOI: 10.1016\/j.dib.2022.108644<\/li>\n
    5. \u0415\u0440\u043c\u0430\u0433\u0430\u043c\u0431\u0435\u0442\u043e\u0432\u0430 \u041c.\u041c., <\/u>\u0410\u0431\u0443\u0433\u0430\u043b\u0438\u0435\u0432\u0430 \u0421.\u0418.,<\/strong> \u0422\u0443\u0440\u0443\u0441\u043f\u0435\u043a\u043e\u0432 \u0415.\u041a., \u0410\u043b\u044c\u043c\u0435\u0440\u0435\u043a\u043e\u0432\u0430 \u0428.\u0421.<\/u> \u041a\u043e\u043d\u0441\u043f\u0435\u043a\u0442 \u0440\u043e\u0434\u0430 Juniperus<\/em> L., \u043f\u0440\u043e\u0438\u0437\u0440\u0430\u0441\u0442\u0430\u044e\u0449\u0435\u0433\u043e \u0432 \u041a\u0430\u0437\u0430\u0445\u0441\u0442\u0430\u043d\u0435.\u00a0Proceedings<\/em><\/strong> on<\/em><\/strong> Applied<\/em><\/strong> Botany<\/em><\/strong>, <\/em><\/strong>Genetics<\/em><\/strong> and<\/em><\/strong> Breeding<\/em><\/strong> (\u0422\u0440\u0443\u0434\u044b \u043f\u043e \u043f\u0440\u0438\u043a\u043b\u0430\u0434\u043d\u043e\u0439 \u0431\u043e\u0442\u0430\u043d\u0438\u043a\u0435, \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0435 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438)<\/em><\/strong> (SJR<\/em>= 0.160, <\/em>percentile<\/em>: 6 — <\/em>Plant<\/em> Science<\/em>, <\/em>Agricultural<\/em> and<\/em> Biological<\/em> Sciences<\/em>). \u2013 2022. \u2013 T. \u2013 \u21163. \u2013 \u0421. 161-170. https:\/\/doi.org\/10.30901\/2227-8834-2022-3-161-170<\/a><\/li>\n
    6. Yermagambetova M.M., Almerekova S.S.,<\/u> Krekova Y., Abugalieva S.I.,<\/strong> Turuspekov Y.K. Genetic variation in populations of Picea schrenkiana<\/em> et CA Mey. based on simple sequence repeat markers. Moscow University Biological Sciences Bulletin<\/em><\/strong> (SJR= 0.189, percentile: 42 — General Agricultural and Biological Sciences, Agricultural and Biological Sciences<\/em>). \u2013 2022. \u2013 Vol. 77(2). \u2013 P. 76-83. DOI : 10.3103\/S0096392522020134<\/li>\n
    7. \u00d6zek, G., Schepetkin I.A., Yermagambetova, M<\/u>., \u00d6zek T., Kirpotina, L.N., Almerekova, S.S.<\/u>, Abugalieva, S.I.,<\/strong> Khlebnikov A.I., Quinn M.T. Innate Immunomodulatory Activity of Cedrol, a Component of Essential Oils Isolated from Juniperus Species \/\/ Molecules <\/em><\/strong>(IF= 4.9, Q2 Biochemistry & Molecular Biology; SJR= 0.705, percentile: 83- Chemistry (miscellaneous), Chemistry)<\/em> \u2013 2021. \u2013 Vol. 26. \u2013 P. 7644. https:\/\/doi.org\/10.3390\/molecules26247644<\/a><\/li>\n
    8. Sumbembayev, A. A., Abugalieva, S. I.,<\/strong> Danilova, A. N., Matveyeva, E. V., & Szlachetko, D. L. A flower morphometry of members of the genus Dactylorhiza<\/em> Necker ex Nevski (Orchidaceae) from the Altai Mountains of Kazakhstan \/\/\u00a0Biodiversitas<\/em><\/strong> (<\/em>SJR= 0.327, percentile: 51 — Plant Science)<\/em>. \u2013 2021. \u2013 Vol.\u00a022<\/em>(8). \u2013 P. 3545-3555 DOI: 10.13057\/biodiv\/d220855<\/li>\n
    9. Almerekova, S.,<\/u> Shchegoleva, N., Abugalieva, S.,<\/strong> & Turuspekov, Y. The molecular taxonomy of three endemic Central Asian species of Ranunculus<\/em> (Ranunculaceae) \/\/\u00a0PLoS ONE<\/em><\/strong> (IF=3.8, Q2\u00a0\u2013\u00a0Multidisciplinary sciences;\u00a0SJR=0.885, percentile: 87\u00a0\u2013\u00a0Multidisciplinary)<\/em>. \u2013 2020. \u2013 Vol.15(10). \u2013 P. e0240121 doi: 10.1371\/journal.pone.0240121<\/li>\n
    10. Almerekova S.,<\/u> Favarisova N., Turuspekov Y., Abugalieva S.<\/strong> * Cross-genera transferability of microsatellite markers and phylogenetic assessment of three Salsola species from Western Kazakhstan \/\/ Proceedings of the Latvian Academy of Sciences, Section B<\/em><\/strong> (SJR=0.143<\/em>,\u00a0percentile: 29-<\/em>Multidisciplinary)<\/em>. \u2013 2020. \u2013 Vol. 74. \u2013 No. 5. \u2013 P. 325-334. doi: 10.2478\/prolas-2020-0049<\/li>\n
    11. Genievskaya Y., Karelova D., Abugalieva S.,<\/strong> Zhao P., Chen G., Turuspekov Y. SSR-based evaluation of genetic diversity in populations of Agriophyllum squarrosum<\/em> and Agriophyllum minus<\/em> Fisch. & Mey. collected in South-East Kazakhstan \/\/ Vavilovskii Zhurnal Genetiki i Selektsii=Vavilov Journal of genetics and Breeding<\/em><\/strong> (IF=0.9, Q3\u00a0\u2013 <\/em>Agriculture,Multidisciplinary sciences;\u00a0SJR=0.223, percentile: 46 \u2013General Agricultural and Biological Sciences<\/em>). \u2013 2020. \u2013 Vol. 24. \u2013 No. 7. \u2013 P. 697-704. DOI: 10.18699\/VJ20.664.<\/li>\n
    12. \u0410\u043b\u044c\u043c\u0435\u0440\u0435\u043a\u043e\u0432\u0430 \u0428.\u0421.,<\/u> \u0410\u0431\u0443\u0433\u0430\u043b\u0438\u0435\u0432\u0430 \u0421.\u0418., *<\/strong> \u041c\u0443\u0445\u0438\u0442\u0434\u0438\u043d\u043e\u0432 \u041d.\u041c. Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan \/\/ Vavilov Journal of Genetics and Breeding (\u0412\u0430\u0432\u0438\u043b\u043e\u0432\u0441\u043a\u0438\u0439 \u0436\u0443\u0440\u043d\u0430\u043b \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0438 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438)<\/em><\/strong> (IF=0.9, Q3\u00a0\u2013 <\/em>Agriculture,Multidisciplinary sciences;\u00a0SJR=0.223, percentile: 46 \u2013General Agricultural and Biological Sciences)<\/em> \u2013 2018. \u2013 \u0422. 22(2). \u2013 \u0421.285-290. DOI: http:\/\/dx.doi.org\/10.18699\/VJ18.362<\/a><\/li>\n
    13. Turuspekov , Genievskaya Y., Baibulatova A., Zatybekov A., Kotuhov Y., Imanbayeva A., Abugalieva S.<\/strong>* Phylogenetic taxonomy of Artemisia<\/em> L. species from Kazakhstan based on mat<\/em>K analyses \/\/ Proceedings of the Latvian Academy of Sciences. Section B.<\/em><\/strong> (SJR=0.127<\/em>,\u00a0percentile: 19-<\/em>Multidisciplinary)<\/em>. \u2013 2018. \u2013 Vol. 72, Issue 1, P. 29-37, ISSN (Online) 1407-009X, DOI: https:\/\/doi.org\/10.1515\/prolas-2017-0068<\/a>.<\/li>\n
    14. Genievskaya Y., Abugalieva S.,<\/strong> Zhubanysheva A., Turuspekov Y. Morphological description and DNA barcoding study of sand rice (Aqriophyllum squarrosum<\/em>, Chenopodiaceae) collected in Kazakhstan \/\/ BMC Plant Biology<\/em><\/strong> (IF=5.761, Q1 \u2013 Plant sciences; SJR=1.159, percentile: 92-Plant Science<\/em>). \u2013 2017. \u2013 Vol.17 (S.1). \u2013 \u0420. 1-8. doi: 10.1186\/s12870-017-1132-1<\/li>\n
    15. Almerekova Sh.,<\/u> Mukhitdinov N., Abugalieva S.<\/strong> Phylogenetic study of the endemic species Oxytropis almaatensis<\/em> (Fabaceae) based on nuclear ribosomal DNA ITS sequences \/\/ BMC Plant biology<\/em><\/strong> (IF=5.9 Q1 \u2013 Plant sciences; SJR=1.159, percentile: 92-Plant Science<\/em>). \u2013 2017. \u2013 Vol.17 (Suppl.1). \u2013 \u0420. 19-27. doi.org\/10.1186\/s12870-017-1128-x;<\/li>\n
    16. \u0422\u0443\u0440\u0443\u0441\u043f\u0435\u043a\u043e\u0432 \u0415.\u041a., \u0410\u0431\u0443\u0433\u0430\u043b\u0438\u0435\u0432\u0430 \u0421.\u0418.,<\/strong> \u0410\u043b\u044c\u043c\u0435\u0440\u0435\u043a\u043e\u0432\u0430 \u0428.\u0421.<\/u> \u0410\u0432\u0442\u043e\u0440\u0441\u043a\u043e\u0435 \u0441\u0432\u0438\u0434\u0435\u0442\u0435\u043b\u044c\u0441\u0442\u0432\u043e \u043e \u0432\u043d\u0435\u0441\u0435\u043d\u0438\u0438 \u0441\u0432\u0435\u0434\u0435\u043d\u0438\u0439 \u0432 \u0433\u043e\u0441\u0440\u0435\u0435\u0441\u0442\u0440 \u043f\u0440\u0430\u0432 \u043d\u0430 \u043e\u0431\u044a\u0435\u043a\u0442\u044b, \u043e\u0445\u0440\u0430\u043d\u044f\u0435\u043c\u044b\u0435 \u0430\u0432\u0442\u043e\u0440\u0441\u043a\u0438\u043c \u043f\u0440\u0430\u0432\u043e\u043c \u2013\u043d\u0430 \u0431\u0430\u0437\u0443 \u0434\u0430\u043d\u043d\u044b\u0445 \u00ab\u0411\u0438\u043e\u0440\u0430\u0437\u043d\u043e\u043e\u0431\u0440\u0430\u0437\u0438\u0435 \u0444\u043b\u043e\u0440\u044b \u041a\u0430\u0437\u0430\u0445\u0441\u0442\u0430\u043d\u0430\u00bb (\u211620035 \u043e\u0442 01.09.2021),\u00a0http:\/\/www.kazflora.kz\/<\/a>.<\/li>\n
    17. \u0410\u043c\u0430\u043b\u043e\u0432\u0430 \u0410.<\/u>, \u041a\u0443\u0440\u043c\u0430\u043d\u0431\u0430\u0435\u0432\u0430 \u041c., \u0422\u0443\u0440\u0443\u0441\u043f\u0435\u043a\u043e\u0432 \u0415., \u0418\u0432\u0430\u0449\u0435\u043d\u043a\u043e \u0410.,
      \n\u0410\u0431\u0438\u0434\u043a\u0443\u043b\u043e\u0432\u0430, \u041a. (2018). \u041e\u043d\u0442\u043e\u0433\u0435\u043d\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0441\u0442\u0440\u0443\u043a\u0442\u0443\u0440\u0430 \u0446\u0435\u043d\u043e\u043f\u043e\u043f\u0443\u043b\u044f\u0446\u0438\u0439 Tulipa<\/em> \u043e<\/em>strowskiana<\/em> Regel \u0432 \u0417\u0430\u0438\u043b\u0438\u0439\u0441\u043a\u043e\u043c \u0410\u043b\u0430\u0442\u0430\u0443. \u0412\u0435\u0441\u0442\u043d\u0438\u043a \u041a\u0430\u0437\u041d\u0423. \u0421\u0435\u0440\u0438\u044f \u044d\u043a\u043e\u043b\u043e\u0433\u0438\u0447\u0435\u0441\u043a\u0430\u044f, 56(3), 101-114<\/li>\n<\/ol>\n

      \u00a0<\/strong><\/p>\n

      Results for 2024:<\/strong><\/p>\n

        \n
      1. Collection of plants of Allium<\/em> species populations and replenishment of the DNA collection collected in the territory of southern and southeastern Kazakhstan has begun. As a result of expeditionary trips to the regions of southern and southeastern Kazakhstan, leaf material of 79 samples of 6 species (7 populations) of Allium<\/em> was collected. The designed and scanned herbarium material was deposited in the database on the biodiversity of the wild flora of Kazakhstan IPBB KazFlora.<\/li>\n
      2. Determination of the population structure and study of intra- and interspecific genetic diversity of Kazakhstani species of the genus Allium<\/em> have begun. The study of intra- and interspecific genetic diversity of species of the genus Allium<\/em> collected in the territory of southern and southeastern Kazakhstan was carried out using SSR markers. Work was carried out on DNA extraction, optimization of PCR conditions and analysis of samples of collected onion populations using SSR markers.<\/li>\n
      3. Determination of nucleotide sequences of DNA markers of the nuclear and chloroplast genome of Allium<\/em> species and reconstruction of the phylogenetic tree of the genus have been started. Determination of nucleotide sequences of DNA markers of the nuclear and chloroplast genome of Allium<\/em> species has been started. The ITS marker was chosen for the nuclear genome, and the matK and rbcL markers were chosen for the chloroplast genome. The study of 2 species by ITS, matK and rbcL markers was carried out.<\/li>\n<\/ol>\n

        \u00a0<\/strong><\/p>\n

        Results for 2025<\/strong><\/p>\n

        In 2025, comprehensive field, molecular genetics, and biochemical studies of wild-growing species of the genus Allium<\/em> were carried out within the framework of the project.<\/p>\n

        Field research and collection<\/em>. In 2025, natural populations of 12 wild Allium<\/em> species were collected, including A. caesium, A. eriocoleum, A. inconspicuum, A. inops, A. karataviense, A. protensum, A. turkestanicum, A. atrosanguineum, A. montanostepposum, A. oreoprasum, A. tianschanicum,<\/em> and A. tulipifolium<\/em>. In total, 24 populations were studied, and 506 plant material samples were collected, allowing for a substantial expansion of the project\u2019s genetic collection.<\/p>\n

        Biochemical studies<\/em>. Leaf material of the widely distributed species A. karataviense<\/em> and A. turkestanicum<\/em> was dried for subsequent investigation of biochemical diversity. A literature review was conducted to identify target compounds and select optimal methodologies. Extracts from the leaf material of the indicated species were obtained.<\/p>\n

        Microsatellite (SSR) analysis<\/em>. Genotyping was performed for two populations of Allium montanostepposum<\/em> using 13 SSR markers, of which 5 were polymorphic, and for five populations of Allium karataviense<\/em> using 21 SSR markers, of which 10 showed polymorphism. The obtained data will be used to assess intra- and interspecific genetic diversity of Allium<\/em> species studied within the project.<\/p>\n

        Sequencing of nuclear and chloroplast DNA markers<\/em>. Work on the analysis of nucleotide sequences of nuclear (ITS) and chloroplast (matK, rbcL) DNA markers was continued. In 2025, samples of eight Allium<\/em> species were sequenced, including A. montanostepposum, A. karataviense, A. turkestanicum, A. eriocoleum, A. inconspicuum, A. inops, A. protensum,<\/em> and A. tulipifolium<\/em>.<\/p>\n

        Chloroplast genome analysis.<\/em> Sequencing and annotation of chloroplast genomes of A. microdictyon, A. atrosanguineum, A. korolkowii,<\/em> and A. sabulosum<\/em> were carried out. In each genome, 133 genes were annotated, including protein-coding, tRNA, and rRNA genes. The obtained data are planned to be used for comparative analysis of chloroplast genome structure and reconstruction of phylogenetic relationships within the genus Allium<\/em>.<\/p>\n

        Information for potential users<\/strong><\/p>\n

        The data obtained during the project are of interest to specialists in botany, molecular genetics, plant taxonomy, and plant biotechnology. The resulting collection of wild species of the genus Allium<\/em>, the results of SSR genotyping, sequencing of nuclear and chloroplast DNA markers, and annotated chloroplast genomes can be used to assess genetic diversity, clarify taxonomic status, and reconstruct phylogenetic relationships within the genus. Biochemical extracts and analytical data hold promise for further phytochemical and pharmacognostic research. The project’s results can be applied in fundamental and applied scientific research, educational processes, and in the preparation of scientific publications and subsequent research programs.<\/p>\n

         <\/p>\n

         <\/p>\n

        Publications 2025<\/strong><\/p>\n

        Abstracts of the international conference <\/em><\/strong>(indicating the form of the report<\/em>)<\/strong><\/p>\n

          \n
        1. Abugalieva S., Almerekova Sh., Yermagambetova M., Turuspekov Y. Characterization of the plastid genomes of Allium<\/em> species from Kazakhstan \/\/ The 11th International Conference on Agricultural and Biological Sciences (ABS 2025). Matsue, Japan, July 21-24, 2025. (poster)<\/li>\n
        2. Ibraimov A., Almerekova S., Abugalieva S. Complete plastome sequence of Allium microdictyon<\/em> and its characteristics. \/\/ 3rd International Symposium on the Frontiers of Plant Diversity Conservation Research on the Pan-Third Pole Region (PDCR 2025): Proceedings of the international symposium \/ edited by prof. Y.K. Turuspekov, prof. S.I. Abugalieva. \u2013 Almaty: IPBB, 2025. \u2013 p. 52. (poster)<\/li>\n<\/ol>\n

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          Brief description of the project (2024-2026) \u00a0 Project title: IRN AP23490860 \u00abBiodiversity of wild species of the genus Allium in southern and southeastern Kazakhstan\u00bb. \u00a0 Relevance.\u00a0Allium L. is one of the largest genera in the tribe Allieae Dumort., and includes more than 1000 species. The primary center of biodiversity of the genus is Central Asia, including Kazakhstan. There are at<\/p>\n

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