{"id":24862,"date":"2025-12-25T12:34:53","date_gmt":"2025-12-25T06:34:53","guid":{"rendered":"https:\/\/ipbb.kz\/eng\/?page_id=24862"},"modified":"2025-12-25T12:34:53","modified_gmt":"2025-12-25T06:34:53","slug":"ap-26103455-superviser-zhambakin-k","status":"publish","type":"page","link":"https:\/\/ipbb.kz\/eng\/ap-26103455-superviser-zhambakin-k\/","title":{"rendered":"AP 26103455 (superviser Zhambakin K.)"},"content":{"rendered":"\n<p class=\"has-text-align-center\"><strong>Brief description of the project<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center\">(2025-2027)<\/p>\n\n\n\n<p><strong>Project title<\/strong><strong>:<\/strong> IRN AP 26103455 \u00abEvaluation of the ecological state of soils in the zones of technogenic pollution of metallurgical enterprises by studying their enzymatic activity and microbiome composition\u00bb.<\/p>\n\n\n\n<p><strong>Relevance.&nbsp;<\/strong><\/p>\n\n\n\n<p>Under conditions of intensive technogenic impact, the application of approaches aimed at assessing the degree of soil degradation under natural functioning conditions is of particular importance. Given the high adaptive capacity of microorganisms to changing environmental conditions, it is crucial to investigate soil microorganisms that function in close proximity to zones affected by technogenic pollution.<\/p>\n\n\n\n<p>The main research approaches include:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>qualitative and quantitative analysis of pollutants in industrially contaminated areas;<\/li>\n\n\n\n<li>assessment of soil enzymatic activity as an indicator of ecological status;<\/li>\n\n\n\n<li>determination of molecular and genetic mechanisms underlying the resistance of soil microorganisms to technogenic factors;<\/li>\n\n\n\n<li>isolation and characterization of heavy metal\u2013resistant microbial strains with potential application in the bioremediation of technogenically impacted areas.<\/li>\n<\/ul>\n\n\n\n<p><strong>The goal of the project<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>To assess the ecological status of soils in industrially contaminated zones of the Ust-Kamenogorsk Metallurgical Complex (UMC) and the Ulba Metallurgical Plant (UMP) through the determination of pollutant composition, microbial community structure, and biochemical activity of the soil microbiome.<\/p>\n\n\n\n<p><strong>Expected results<\/strong><strong>:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Qualitative and quantitative composition of pollutants in soils affected by industrial contamination from UKMC and UMP will be determined;<\/li>\n\n\n\n<li>Spatial interpolation maps of heavy metal distribution in industrial contamination zones will be generated using QGIS 3.36.2;<\/li>\n\n\n\n<li>Soil enzymatic activity will be evaluated as an indicator of ecological condition in technogenically impacted and control areas;<\/li>\n\n\n\n<li>Molecular and genetic characteristics of soil microorganism resistance to technogenic stressors will be identified;<\/li>\n\n\n\n<li>Heavy metal\u2013resistant microbial strains will be isolated and characterized for potential application in bioremediation of contaminated areas;<\/li>\n\n\n\n<li>A model of microbial ecological succession will be developed based on comparative analysis of the obtained results;<\/li>\n\n\n\n<li>One Doctor of Philosophy (PhD) will be trained;<\/li>\n\n\n\n<li>Research results will be published in peer-reviewed scientific journals.<\/li>\n<\/ol>\n\n\n\n<p><strong>Scientific Supervisor of the project<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>Zhambakin Kabyl Zhap\u0430rovich, Doctor of Biological Sciences, Professor<\/p>\n\n\n\n<p><strong>Research group<\/strong><strong>:&nbsp;<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Zhambakin Kabyl Zhap\u0430rovich \u2014 Professor, Doctor of Biological Sciences, Academician of the National Academy of Sciences of the Republic of Kazakhstan<\/li>\n\n\n\n<li>Shamekova Malika Khabidulaevna \u2014 PhD, Associate Professor<\/li>\n\n\n\n<li>Daurov Dias Lamzarovich \u2014 Master\u2019s degree<\/li>\n\n\n\n<li>Abilda Zhanar Kassymkhankyzy \u2014 Master\u2019s degree<\/li>\n\n\n\n<li>Kanat Rakhim \u2014 Bachelor\u2019s degree<\/li>\n\n\n\n<li>Sapakhova Zagipa Beisenovna \u2014 PhD, Associate Professor<\/li>\n\n\n\n<li>Sadykova Assel Dulatovna \u2014 Higher education<\/li>\n\n\n\n<li>Choi Khanylbek \u2014 Bachelor\u2019s degree<\/li>\n<\/ol>\n\n\n\n<p><strong>List of publications of the project\u2019s participants<\/strong><strong><\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Method for phytoremediation of soil. Innovation Patent of the Republic of Kazakhstan No. 2008\/1108.1 (06.10.2008).<\/li>\n\n\n\n<li>Method for identifying pesticide-contaminated soils. Innovation Patent of the Republic of Kazakhstan No. 2008\/1260.1 (14.11.2008).<\/li>\n\n\n\n<li>Method for bioindication of plant resistance to pesticides. Innovation Patent of the Republic of Kazakhstan No. 2008\/1261.1 (14.11.2008).<\/li>\n\n\n\n<li>Method for utilization of pesticide-contaminated phytomass. Application No. 17069 (10.10.2011).<\/li>\n\n\n\n<li>Digital platform for seed quality control \u201cE-Tukym\u201d. Certificate No. 45900 (16.05.2024).<\/li>\n\n\n\n<li>Accounting system for seed potatoes and fruit planting material in Kazakhstan. Certificate No. 40039 (31.10.2023).<\/li>\n<\/ol>\n\n\n\n<p>1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurov D., Daurova A., Sapakhova Z., Kanat R., Akhmetzhanova D., Abilda Z., Toishimanov M., Raissova N., Otynshiyev M., Zhambakin K., Shamekova M. (2024). The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers. Agronomy, 14(8), 1782. https:\/\/doi.org\/10.3390\/agronomy14081782. \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q1, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 84.<\/p>\n\n\n\n<p>2.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurov D., Lim Y.H., Park S.U., Kim Y.H., Daurova A., Sapakhova Z., Zhapar K., Abilda Z., Toishimanov M., Shamekova M., Zhambakin K., Kim H.S., Kwak S.S. Selection and characterization of lead-tolerant sweetpotato cultivars for phytoremediation. Plant Biotechnology Reports. 2024. https:\/\/doi.org\/10.1007\/s11816-024-00900-w. \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q2, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 69.<\/p>\n\n\n\n<p>3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Toishimanov M., Abilda Z., Daurov D., Daurova A., Zhapar K., Sapakhova Z., Kanat R., Stamgaliyeva Z., Zhambakin K., Shamekova M. Phytoremediation properties of sweet potato for soils contaminated by heavy metals in South Kazakhstan. Applied Sciences. 2023. 13. 9589. https:\/\/doi.org\/10.3390\/app13179589. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 2.44, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q2, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 83.<\/p>\n\n\n\n<p>4.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurov D., Zhambakin K., Shamekova M. Phytoremediation as a way to clean technogenically polluted areas of Kazakhstan. Brazilian journal of biology = Revista brasleira de biologia. 2023. 83. e271684. https:\/\/doi.org\/10.1590\/1519-6984.271684. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 3.05\u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q3, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 61.<\/p>\n\n\n\n<p>4&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Sapakhova Z., Raissova N., Daurov D., Zhapar K., Daurova A., Zhigailov A., Zhambakin K., Sweet potato as a key crop for food security under the conditions of global climate change: A Review. Plants. 2023. 12. 2516. https:\/\/doi.org\/10.3390\/plants12132516. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 2.55, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q1, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 83.<\/p>\n\n\n\n<p>5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Sapakhova Z., Islam K.R, Toishimanov M., Zhapar K., Daurov D., Daurova A., Raissova N., Kanat R., Shamekova M., Zhambakin K. Mulching to improve sweet potato production. Journal of Agriculture and Food Research. 2024. 15. 101011. https:\/\/doi.org\/10.1016\/j.jafr.2024.101011. \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q1, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 78.<\/p>\n\n\n\n<p>6&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Gritsenko D., Daurova A., Pozharskiy A., Nizamdinova G., Khusnitdinova M., Sapakhova Z., Daurov D., Zhapar K., Shamekova M., Kalendar R., Zhambakin K. Investigation of mutation load and rate in androgenic mutant lines of rapeseed in early generations evaluated by high-density SNP genotyping. Heliyon. 2023. 9(3). e14065. https:\/\/doi.org\/10.1016\/j.heliyon.2023.e14065. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 0.41, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q2, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 86.<\/p>\n\n\n\n<p>7&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurov D., Argynbayeva A., Daurova A., Zhapar K., Sapakhova Z., Zhambakin K., Shamekova M. Monitoring the spread of potato virus diseases in Kazakhstan. American Journal of Potato Research. 2023. 100(1). 63-70. https:\/\/doi.org\/10.1007\/s12230-022-09895-y. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 0.55, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q3, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 76.<\/p>\n\n\n\n<p>8&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurova A.K., Volkov D.V., Daurov D.L., Zhapar K.K., Sapakhova Z.B., Shamekova M.Kh., Zhambakin K.Zh. Improvement of breeding-valuable traits of rapeseed (Brassica napus) using mutagenesis. International Journal of Agriculture and Biology. 2022. 28(4). 219-227. https:\/\/doi.org\/10.17957\/IJAB\/15.1973. \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q3, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 56.<\/p>\n\n\n\n<p>9&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurova A., Daurov D., Volkov D., Zhapar K., Raimbek D., Shamekova M., Zhambakin K. Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits. Oilseeds and fats, Crops and Lipids (OCL). 2020. 27. 45. https:\/\/doi.org\/10.1051\/ocl\/2020041. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 0.33, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q2, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 68.<\/p>\n\n\n\n<p>10&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurov D., Daurova A., Karimov A. Tolegenova D., Volkov D., Raimbek D., Zhambakin K., Shamekova M. Determining effective methods of obtaining virus-free potato for cultivation in Kazakhstan. American Journal of Potato Research. 2020. 97. 367-375. https:\/\/doi.org\/10.1007\/s12230-020-09787-z. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 0.40, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q3, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 76.<\/p>\n\n\n\n<p>11&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Daurova A., Daurov D., Volkov D., Karimov A., Abai Z., Raimbek D., Zhapar K., Zhambakin K., Shamekova M. Mutagenic treatment of microspore-derived embryos of turnip rape (Brassica rapa) to increase oleic acid content. Plant Breeding. 2020. 139(5). 916-922. https:\/\/doi.org\/10.1111\/pbr.12830. \u0418\u043d\u0434\u0435\u043a\u0441 \u0446\u0438\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f (FWCI)\u2013 0.35, \u041a\u0432\u0430\u0440\u0442\u0438\u043b\u044c \u2013 Q2, \u041f\u0440\u043e\u0446\u0435\u043d\u0442\u0438\u043b\u044c \u2013 73.<\/p>\n\n\n\n<p><strong>Results for 202<\/strong><strong>5<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>Spring and autumn field expeditions were conducted to collect soil samples. Qualitative and quantitative analyses of soil pollutants were performed. Cadmium, copper, and zinc were identified as the primary pollutants based on significant exceedances of maximum permissible concentrations (MPCs) detected through chemical soil analysis.<\/p>\n\n\n\n<p>The highest exceedances were observed for cadmium (2\u201356 MPC), followed by zinc (2\u201350 MPC), while copper exceedances ranged from 1.2 to 25 MPC.<\/p>\n\n\n\n<p>Catalase activity was determined by titration of hydrogen peroxide using 0.02 M KMnO\u2084. Soil enzymatic activity corresponded closely to contamination levels:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>severe contamination \u2014 approximately 40% activity;<\/li>\n\n\n\n<li>moderate contamination \u2014 50\u201355%;<\/li>\n\n\n\n<li>low contamination \u2014 approximately 55%;<\/li>\n\n\n\n<li>control sites \u2014 highest activity (~65%).<\/li>\n<\/ul>\n\n\n\n<p>A soil-free blank was used as the reference standard.<\/p>\n\n\n\n<p>At the current stage, DNA was extracted from soil samples using the DNeasy PowerSoil Kit and further purified with AMPure XP magnetic beads to achieve the required DNA quality. Sequencing was performed using the PromethION platform. Soils from areas with low, moderate, high, and very high ecological risk were sequenced.<\/p>\n\n\n\n<p>Bacteria belonging to the family <em>Pseudomonadaceae<\/em>, genus <em>Pseudomonas<\/em>, specifically <em>Pseudomonas mendocina<\/em> NK-01, were identified. These aerobic bacteria exhibit relatively low pathogenicity compared to closely related taxa. In addition, poxviruses were detected, which are animal viruses with potential pathogenicity for humans.<\/p>\n\n\n\n<p><strong>Publications (202<\/strong><strong>5<\/strong><strong>):<\/strong><strong> <\/strong>no publications<strong><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Brief description of the project (2025-2027) Project title: IRN AP 26103455 \u00abEvaluation of the ecological state of soils in the zones of technogenic pollution of metallurgical enterprises by studying their enzymatic activity and microbiome composition\u00bb. Relevance.&nbsp; Under conditions of intensive technogenic impact, the application of approaches aimed at assessing the degree of soil degradation under natural functioning conditions is of<\/p>\n<div class=\"h10\"><\/div>\n<p><a class=\"more-link1\" href=\"https:\/\/ipbb.kz\/eng\/ap-26103455-superviser-zhambakin-k\/\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-24862","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/pages\/24862","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/comments?post=24862"}],"version-history":[{"count":1,"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/pages\/24862\/revisions"}],"predecessor-version":[{"id":24863,"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/pages\/24862\/revisions\/24863"}],"wp:attachment":[{"href":"https:\/\/ipbb.kz\/eng\/wp-json\/wp\/v2\/media?parent=24862"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}