AP 14870355 (superviser Uteulin K.)

(2022-2024)

 Project title:   IRN АР14870355 «Conservation and use of Kazakhstan genetic resources of dandelion kok-saghyz (Taraxacum kok-saghyz L.E. Rodin) — a source of high-quality rubber».

Relevance.

Natural rubber (NC) is a strategically important material in the industrial production of the whole world, NC is used in the rubber industry, in the manufacture of a wide range of goods — shoes, clothing, catheters, surgical gloves, tires for airplanes and cars and other products.

The global NC market in 2016 amounted to approximately $24 mlr, with the volume of NC consumption — 12.9 million tons, and by 2023 its projected consumption will increase to 16.5 million tons (International Group for the Study of Rubber – IRSG).

The main source of NK are plantations of the rubber tree Hevea (Hevea brasiliensis Willd. ex A.Juss.) Müll.Arg.), grown in the tropical, humid climate of the countries of Southeast Asia. However, due to the threat of destruction of hevea plantations in Southeast Asia by diseases, currently in temperate countries (Canada, USA, EU countries, Russian Federation, Kazakhstan and others), dandelion kok-sagyz (Taraxacum kok-saghyz L.E. Rodin) is introduced into culture — a source of natural rubber, in quality not inferior to the reference rubber of hevea.

In situ populations of T. kok-saghyz are located in Kazakhstan’s intermountain valleys of the Tien Shan. The problem is that under the conditions of introduction, the viability and content of rubber in the roots of T. kok-saghyz decrease. In this regard, studies of the natural soil habitat of T. kok-saghyz – soils in situ populations are relevant.

1. kok-saghyz is being actively studied in foreign scientific centers. In this regard, the work to ensure the demand of scientific.           

The goal of the project: To develop methods of conservation and sustainable use of Kazakhstan genetic resources of rubber dandelion kok-saghyz (Taraxacum kok-saghyz L.E. Rodin) under in situ conditions.

Expected results:

1. In situ soil environment of T. kok-saghyz populations (Jalauly, Kegen, Saryzhaz and Saline Lake) will be studied,

2.An in situ field genebank of T. kok-saghyz will be organized to distribute seeds to research centers.

3. The intellectual property obtained by the results of the project will be protected.

Scientific Supervisor of the project:

Uteulin K. R, Doctor of Biological Sciences, Associate Professor.

Author ID Scopus 6602865844

Researcher ID Web of Science L-3508-2018

E.mail: gen_uteulink@mail.ru

Research group:

Suleimenov B.U.,  Doctor of Agricultural Sciences, Associate Professor

Author ID Scopus: 55951606700

ORCID ID https://orcid.org/0000-0003-0507-8053

https://kzpatents.com/patents/sulejjmenov-bejjbut-ualihanovich

E.mail:beibuts@mail.

Kuluev, Doctor of Biological Sciences, leading researcher.

Author ID Scopus: 55951606700

ORCID ID https://orcid.org/0000-0003-0507-8053

E.mail: kuluev@bk.ru

Bari G.T., Phd. Young Scientist.

Author ID Scopus: 55951606700

ORCID ID https://orcid.org/0000-0003-0507-8053

E.mail: kuluev@bk.ru

List of publications of the project’s participants (2017-2022)

1. Bari Gabit, Gainullina Karina, Gumerova Gulnar, Uteulin Kairat, Golovanov Yaroslav , Chemeris Aleksey, Kuluev Bulat. «Multilocus DNA polymorphism of some rubber-bearing dandelions of Russia and Kazakhstan.» Genetic Resources and Crop Evolution. (2021). https://doi.org/10.1007/s10722-021-01233-1. Published online 19 June 2021. The Author(s), under exclusive licence to Springer Nature B.V. 2021. Content courtesy of Springer Nature, terms of use apply. Rights reserved. ISSN 09259864, 15735109. H — index 66. SJR 48. Impact factor 1.071. Database   Scopus Q 2. Percentile 67, Scopus.
2. Amerik, A. Yu. , Martirosyan, Yu. Ts., Martirosyan, L. Yu., Goldberg, V. M., Uteulin, K. R. , Varfolomeev, S. D. Molecular Genetic Analysis of Natural Rubber Biosynthesis. Russian Journal of Plant Physiology. 2021. 68(1):31-45. ISSN 10214437, 16083407 DOI10.1134/S1021443721010039. H  Indeks   CJR  0, 42.  CiteScore  1,8.  Database   Scopus  Q2. Percentile 54, Scopus.
3. Aizhan Rakhmetullina, Piotr Zielenkiewicz, Anna Pyrkova, Kairat Uteulin, Anatoliy Ivashchenko. Prediction of characteristics of interactions of miRNA with mRNA of GRAS, ERF, C2H2 genes of   thaliana, O. sativa  and  Z. mays // Current Plant Biology  28 (2021) 100224. https://doi.org/10.1016/j.cpb.2021.100224 Contents lists available at ScienceDirect. journal homepage: www.elsevier.com/locate/cpb Received 29 July 2021; Received in revised form 6 October 2021; Accepted 6 October 2021 Available online 16 October 2021 2214-6628/© 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license Database Scopus Q 2. Percentile 71, Scopus.
4. Kairat R. Uteulin. Reintroduction of kok-saghyz (Taraxacum kok-saghyz Rodin). REVISTA GEINTEC-GESTAO INOVACAO E TECNOLOGIAS (Management, Innovation and Technologies). ISSN: 2237-0722. Vol. 11 No. 2 (2021). P. 240- 249.    https://www.revistageintec.net/index.php/revista/article/view/1657

DOI: https://doi.org/10.47059/revistageintec.v11i2.1657

5. R. Uteulin. Clone Selection of kok-Saghyz (Taraxacum kok-saghyz), A Source of Natural Rubber. Annals of the Romanian Society for Cell Biology. ISSN:1583-6258, Vol. 25, Issue 3, 2021, Pages. 509 – 515. Received 16 February 2021; Accepted 08 March 2021. ISSN 15836258.   http://annalsofrscb.ro/index.php/journal/article/view/1525.
6. R. Uteulin, K.Z. Zambakin. Table of contents and localization of rubber in the roots of kok-saghyz (Taraxacum kok-saghyz Rodin). Reports of the National Academy of sciences of the Republic of Kazakhstan. Volume 6, Number 334 (2020), 42 – 48. ISSN 2224-5227 DOI: 10.32014/2020.2518-1483.134.
7. R. Uteulin, G. T. Bari, A. Zheksenbai. Dandelion kok-saghyz (Taraxacum kok-saghyz L.Rodin) as a one-year culture developed under conditions of south east Kazakhstan. Bulletin the National academy of sciences of the Republic of Kazakhstan . ISSN 1991-3494 Volume 3, Number 385 (2020), 36 – 42 . ISSN 1991-3494. UDC 633.913.322. https://orcid.org/0000-0002-5459-0902. DOI: 10.32014/2020.2518-1467.67.
8. R. Uteulin, K. J. Zhambakin, I. R. Rakhimbaev, I. O. Baitulin, S. K. Mukhambetzhanov The patent No. 862. Breeding achievement — variety of kok-saghyz «Saryzhaz». Date of registration in the State Register of Breeding Achievements of the Republic of Kazakhstan – November 22, 2018. (RSE National Institute of Intellectual Property, Ministry of Justice of the Republic of Kazakhstan). Equivalent to the publications recommended by the Committee on Control in Education and Science of the Ministry of Education and Science of the Republic of Kazakhstan.

Results for 2022:

Task 1. Study of the natural soil habitat of T. kok-saghyz.

Field research

• — In accordance with the work plan of the project, field research was conducted in Kegen district of Almaty region to study landscapes and soils in the habitats of the dandelion coq sagyz.
• — The floodplains of the Kegen River in the areas of Zhalauly and Kegen villages, in the area of groundwater seepage to the north of the village of Saryzhas, and in the area of the dandelion’s habitat were surveyed. Saryzhas, as well as on the coast of the salt lake Tuzkol.

 Eight soil sections were laid, georeferenced using GPS, a description of the relief, vegetation, as well as the morphological structure of soil profiles by genetic horizons, including the characteristics of color, moisture, compaction of the structure, and mechanical composition of the soil (shown below) were carried out. Photographs of soil profiles and their corresponding landscapes were taken. Soil samples were selected for further chemical analyses.

Location of soil sections in the places where kok-saghyz grows

Sampling and chemical analysis of soils

— Soil samples were collected and chemical analyses of 41 soil samples were conducted in the Kok-Sagyz growing areas and 16 samples in the foothill plain of Zailiisky Alatau.

— The following types of analyses were conducted: humus, gross nitrogen, CO2, pH, absorbed Ca, Mg, Na, K, nutrients (N, P, K), amount and composition of salts, mechanical (granulometric) composition of soils, microelements.

— Territories on foothill plain of Zailiisky Alatau have been surveyed for the choice of sites for laying of experiments in situ, and also carrying out of model experiments in Institute where 3 soil cuts have been put in the areas of approximate conditions of growing kok-sagyz with sampling for chemical analyses, and also the soil in an amount of 75 kg for statement of experiments in Institute.

— Conclusion

— It was established that kok-sagyz grows in sity in conditions of floodplains of intermountain valleys on strongly saline floodplain meadow soils of sulphate-sodium composition with high content of humus (3-9 %) and in alkaline environment (рН = 8,8 — 9,6) and very high content of nutrients (N, P, K).

Problem 2: Organization of a field genebank of T. kok-saghyz species under in situ conditions.

Reintroduction of T. kok-saghyz to organize a field genebank under in situ conditions.

Publications (2022):

1. Bari GT, Zhanbyrbaev EA, Jantasov SK, Uteulin KR Identification and production of inulin from the roots of kok-saghyz (Taraxacum kok-saghyz Rodin) // 3i: intellect, idea, innovation. Kostanay Regional University named after Akhmet Baitursynov. №3. 2022. — С. 79-85. KKSON MNVO RK.

Results for 2023-2024:

For the first time, a field gene bank for kok-saghyz has been established in its natural habitat in the intermountain valleys of the Tien Shan in Kazakhstan. The current state of the soils in the kok-saghyz natural habitat area has been studied. The in situ field gene bank of kok-saghyz has been created specifically as a source of elite seeds to replenish Kazakhstan and international ex situ gene banks, research, breeding programs, and other consumers. The in situ seed productivity of the kok-sagyz genebank is 21,600 seeds per year. Seeds were sent to scientific centers. Data on the in situ genebank is documented. To increase the productivity of the introduced kok-sagyz, a technology for obtaining a complex fertilizer has been developed.

The in situ field gene bank of kok-saghyz is an implemented technological innovation ensuring the conservation and use of Kazakhstan’s genetic resources of kok-saghyz, a method of protecting this rare species included in the Red Book. Convention on Biological Diversity Goals: The project has achieved the goals of the Convention on Biological Diversity: conservation of biological diversity and sustainable use of its components. The benefits of using genetic resources may include monetary and non-monetary benefits. The project’s research results have been applied to address scientific and technological development tasks of the Republic of Kazakhstan.

Publications (2023-2024):

1. Uteulin K., Suleimenov B., Pachikin K. The Soils of Natural (In Situ) Coenopopulations of Taraxacum kok-saghyz L.E. Rodin in Kazakhstan. Agronomy 2023, 13(11), 2737; https://doi.org/10.3390/agronomy13112737. Published: 30 October 2023, Q1, percentile 84.
2. Bulat Kuluev, Kairat Uteulin, Gabit Bari, Elvina Baimukhametova, Khalit Musin, Alexey Chemeris. Molecular Genetic Research and Genetic Engineering of Taraxacum kok-saghyz L.E. Rodin. 2023, 12(8), 1621; https://doi.org/10.3390/plants12081621 (registering DOI). Q 1. percentile 86.
3. Uteulin K. Kazakh populations of kok—sagyz dandelion (Taraxacum kok-saghyz L.E. Rodin). «Bulletin of the Karaganda University. Series «Biology, medicine, geography«». – 2023. – Vol.2, №110. – Р. 151- 161. https://doi.org//10.31489/2023BMG2/.
4. Uteulin K. Seed germination and seedling development of rubber dandelion kok-saghyz (Taraxacum kok-saghyzE. Rodin) on soil samples in situ populations: «Salt Lake», «Saryzhaz», «Kegen». «Bulletin of the Karaganda University. Series «Biology, medicine, geography«». – 2023. – № 3. – Р. 196-205. https://doi.org// 10.31489/2023BMG3/196-205.
5. Patent RK for Invention №36776, 10.2024 «Method of pre-sowing treatment of kok-sagyz seeds (Taraxacum kok-saghyz L.E. Rodin)». Authors: Bari G.T., Uteulin K.
6. Uteulin K. The monograph «Kok sagyz. The current state. Introduction to culture«. – 2024. — Аlmaty, Zhebe-design. – 120 р.