Brief information about the project
(project duration 27 month, 2020-2022 yy.)
Project title: IRN АР08856576 “Creating source material of turnip rape (Brassica rapa) to create new cultivars for Northern Kazakhstan”.
Oilseed cultivation areas are expanding in Kazakhstan every year due to cost-effectiveness of oil crops. At the same time, there is an apparent shortage in domestic oilseed cultivars, especially in canola. Moreover, local canola hybrids do not even exist. This situation is obviously contraindicating country’s food safety. Canola is a tradename of rapeseed (Brassica napus) and turnip rape (Brassica rapa). Unlike conventional rapeseed oil, canola oil does not contain glucosinolates and erucic acid which may be toxic to human and animals. Growth conditions in main regions of spring rapeseed cultivation in the north of the country are changing. Firstly, unstable rainfalls and increasing air temperature during growing season are becoming more common, which is critical for the region of rapeseed cultivation, since the crop is being grown without irrigation. In addition, increase in rapeseed pests due to expanding growth areas has led to an increase in pesticide expenses. As a consequence, there is an urgent need in new canola varieties with resistance to abiotic stress factors and shorter growing season. Shorter growing season and better cold hardiness compared to rapeseed would give an opportunity to avoid critical conditions at early growth stages during intensive exposure to pests. Turnip rape (Brassica rapa) is the plant that fits into these characteristics. In Kazakhstan, turnip rape is almost not grown. Therefore, this project is dedicated to solve the issue of creating local turnip rape cultivars, as well as hybrids in the future.
Goal of the project: To obtain productive turnip rape lines resistant to abiotic stress factors for introducing in breeding process in order to create first domestic cultivars.
Expected results:
- The working collection will contain no less than 30 cultivars and lines of spring turnip rape with high productivity and abiotic stress tolerance.
- Doubled haploid plants of spring turnip rape hybrids.
- Mutant doubled haploid lines of spring turnip rape.
- 1) Doubled haploid hybrid lines of spring turnip rape. 2) Optimized protocol for obtaining doubled haploids of spring turnip rape.
- Preliminarily selected (no less than 30) lines on cold, drought tolerance and on oil fatty acid composition for testing under conditions of Northern Kazakhstan.
- No less than 10 promising lines according to a complex of breeding traits for further breeding and creating domestic cultivars.
Scientific supervisor of the project:
Zhambakin Kabyl Zhaparovich, general director of IPBB, Doctor of Biological Science (specialty in Biotechnology), Professor (specialty in Biotechnology), Academician of KR NAS, h-index 3, 4 candidate dissertations and 3 PhD theses were defended under his supervision.
(https://www.scopus.com/authid/detail.uri?authorId=55331576800; https://app.webofknowledge.com/author/record/3844956)
Member in the research group:
Shamekova Malika Khabidullayevna: higher education (specialty in Animal Science), MSc (specialty in Agricultural Ecology), PhD (specialty in Ecology), Associated Professor (specialty in Biology), 15 years of research experience, fits the project profile, h-index 4, 1 PhD thesis was defended under her supervision.
(https://www.scopus.com/authid/detail.uri?authorId=55617198500; https://app.webofknowledge.com/author/record/4172091).
Daurov Dias Lamzarovich: BSc (specialty in Biotechnology), MSc (specialty in Biotechnology), 7 years of research experience, fits the project profile, h-index 1.
(https://www.scopus.com/authid/detail.uri?origin=resultslist&authorId=57201666405&zone= ; https://app.webofknowledge.com/author/record/35819805?lang=ru_RU&SID=F5UIG1R9xCKg1bvOMMo)
Daurova Ainash Kenenbayevna: BSc (specialty in Biotechnology), MSc (specialty in Biotechnology), 7 years of research experience, fits the project profile, h-index 1.
(https://www.scopus.com/authid/detail.uri?origin=AuthorProfile&authorId=57201673636&zone=; https://app.webofknowledge.com/author/record/10089321?lang=ru_RU&SID=F5UIG1R9xCKg1bvOMMo)
Volkov Dmitriy Vladimirovich: higher education (specialty in Biotechnology), MSc (specialty in Biology), 2nd year PhD student at KazNAU (specialty in Agronomy), 15 years of research experience, fits the project profile, h-index 1. (https://www.scopus.com/authid/detail.uri?authorId=57201665674&eid=2-s2.0-85045629532 https://app.webofknowledge.com/author/record/35259389?lang=ru_RU&SID=F6mGWWnVAVKXP1xQwE2).
Zhapar Kuanysh Kabyl uly: BSc (specialty in Biology), MSc (specialty in Biology), PhD student at KazNAU (specialty in Plant Protection and Quarantine), 7 years of research experience, fits the project profile, h-index 1. (https://www.scopus.com/authid/detail.uri?authorId=57201671071; https://app.webofknowledge.com/author/record/13315794).
Zhizhin Nikolai Ivanovich: higher education (specialty in Chemistry), 15 years of research experience, 4 of which fit the project profile.
List of publications and patents of the project executors for the period from 2015 to 2020.
- Zhambakin, K., Zhapar, K. Current status and prospects of plant biotechnology in Kazakhstan. Plant Biotechnology Report 14, 177–184 (2020). https://doi.org/10.1007/s11816-020-00601-0 IF-1.259, Q-3, процентиль-64
- Daurova Ainash, Daurov Dias, Volkov Dmitriy, Aibek Karimov, Zhandos Abai, Daniyar Raimbek,Kuanish Zhapar, Kabyl Zhambakin, Malika Shamekova. Mutagenic treatment of microspore-derived embryos of turnip rape (Brassica rapa) to increase oleic acid content. Plant Breeding. 2020;00:1–7. https://doi.org/10.1111/pbr.12830 IF – 1.251, Q -3, процентиль- 62
- K.Daurova, D.L.Daurov, D.V.Volkov, M.O.Bakbergenova, Zh.Zhambakin,M.Kh.Shamekova. Production of rapeseed (Brassica napus) interspecific hybrids with rape(Brassica campestris) and mustard (Brassica juncea) // Journal of Biotechnology. – 2017. –V.256. – PS99 http://dx.doi.org/10.1016/j.jbiotec.2017.06.1138 IF- 3.163, Q-2, процентилль-74
- Zhambakin K., Zatybekov A., Volkov D., Shamekova M. Production of rapeseed mutant lines by microspore-derived embryo culture // Journal of biotechnology – 2014. – Vol.185,S,–P.29-30. http://dx.doi.org/10.1016/j.jbiotec.2014.07.100 IF-3.163, Q-2, процентилль-74
- Sartbaeva I.A., Usenbekov B.N., Rysbekova M., Mukhina D.T., Kazkeev K.Z., Zhambakin E.A., Zhanbyrbaev K., Berkimbai A.B., Akhmetova D.S., Meldebekova A.A., Obtaining of Doubled Haploid Lines for Selection of Glutinous Rice. Биотехнология, 2018, Т. 34, № 2, С. 26–36 DOI: 10.21519/0234-2758-2018-34-1-26-36
- Kalendar R, Muterko A, Shamekova M, Zhambakin K. In Silico PCR Tools for a Fast Primer, Probe, and Advanced Searching. Methods in Molecular Biology (Clifton, N.J.) Vol.51, 2 : 147-153. DOI:1007/978-1-4939-7060-5_1
Results obtained for 2020-2021
A working collection of turnip rape has been formed from varieties of Russian and German breeding, from mutant lines of doubled haploids obtained by us in previous studies, as well as lines of doubled haploids of interspecific hybrids of rapeseed with turnip rape.
Samples of the working collection of spring wheat were obtained in vitro and in vivo. Digaploid hybrid plants of spring surepitsa were obtained. Mutant digaploid lines of spring surepitsa were obtained. The protocol of mutagenesis of embryoids from the culture of microspores of spring surepitsa has been optimized. Seeds of doubled haploids of mutant plants of spring surepitsa, as well as their hybrids, were obtained.
