“Genetic diversity and preservation of genetic resources of endemic, rare and industrially important plants in the Republic of Kazakhstan” according to «The intellectual potential of the country» priority for 2015-2017. The head is Turuspekov Y.
Introduction to the problem. Safety instructions. Work process in the lab. Issuance of the course materials.
Day 1. Methods of express DNA extraction for PCR. Preparation of solutions. Disruption of cells and tissues, removing of proteins and impurities. DNA Precipitation.
Day 2. Checking the quality and quantity of DNA. Agarose gel electrophoresis. Spectrophotometer.
Day 3. PCR theory. Formulation of the problem. Ingredients of the reaction. PCR running.
Day 4. Gels filling. Electrophoresis. Gel — documentation system. Documenting of results. Cutting off of DNA-bands from agarose gels.
Day 5. Using of the commercial kits for DNA purification.
Day 6. PCR for DNA sequencing. Purification of PCR products from the excess of dye, primers and other impurities.
Day 7. Introduction to the DNA sequencing machine (DNA sequencer). Start of DNA sequencing.
Day 8. Documentation of the sequencing results. Comparative analysis of nucleotide sequences.
Day 9. Work with application software for cluster analysis of samples. An analysis of massive data for samples genotyping. Results interpretation.
Day 10. Discussion of results . Issuance of certificates.
Абугалиева А.И., Абугалиева С.И., Кворри С.А., Туруспеков Е.К., Чакмак И., Савин Т.В., Ганеев В.А. Содержание Fe, Zn и S в зерне популяции дигаплоидных линий мягкой пшеницы Chinese Spring х SQ1 // Вавиловский журнал генетики и селекции. – 2012. –Т.16, №4/2. – С. 894-900. Импакт-фактор РИНЦ 0.304
Абугалиева С.И., Середа Г.А., Чудинов В.А., Сариев Б.С., Туруспеков Е.К. Анализ хозяйственно-ценных признаков мировой коллекции овса, выращенной в трех различных регионах Казахстана // Труды по прикладной ботанике, генетике и селекции. – 2013. – Т.171. – С. 168-174. Импакт-фактор РИНЦ 0.032
Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan // Генетика. – 2013, том 49, № 2, с. 196-205. Impact factor 0.448
Turuspekov Y., Abugalieva S., Ermekbayev K., Sato K. 2014. Genetic characterization of wild barley populations (Hordeum vulgare ssp. spontaneum) from Kazakhstan based on genome wide SNP analysis. Breeding Science 64:399-403. Impact factor 2.13
Абугалиева С.И., Байбосынова С.М., Кондыбаев А.Б., Подольских А.Н., Туруспеков Е.К. Генетическое и фенотипическое разнообразие коллекции риса в Казахстане // Труды по прикладной ботанике, генетике и селекции. – 2014. – Т.175. 2014. –Т.175., вып. 4. – С. 46-59. Импакт-фактор РИНЦ 0.032
Abugalieva A .I., TuruspekovK., Abugalieva S.I., Savin T.V. Genetic markers and biochemical evaluation in the winter triticale identification and breeding // Communications in Agricultural and Applied Biological Sciences. 2014, 79(4):233-239.
Read the articleTuruspekov Y., Plieske J., Ganal M., Akhunov E., Abugalieva S. Phylogenetic analysis of wheat cultivars in Kazakhstan based on the wheat 90K single nucleotide polymorphism array. Plant Genetic Resources, 2015, available on CJO2015. doi:10.1017/S1479262115000325. Impact factor 0.442
Read the articleVolis S., Ormanbekova D., Yermekbayev, SongM.,Shulgina I. The Conservation Value of Peripheral Populations and a Relationship Between Quantitative Trait and Molecular Variation // Evolutionary Biology. – 2015. – Vol. 42, N4. – P.1-11. Impact factor 2.267
Read the articleVolis S., Ormanbekova D., Yermekbayev K. Role of phenotypic plasticity and population differentiation in adaptation to novel environmental conditions // Ecology and Evolution. – Vol.5, N17. –P. 3818-3829. Impact factor 2.537
Volis S., Ormanbekova D., Yermekbayev K., Song M., Shulgina I. Multi-Approaches Analysis Reveals Local Adaptation in the Emmer Wheat (Triticum dicoccoides) at Macro- but not Micro-Geographical Scale // PLoS ONE. – 2015. – Vol.10, N3. – P. e0121153. Impact factor 3.234.
Абугалиева С.И., Ермекбаев К.А., Волкова Л.А., Орманбекова Д.Е., Туруспеков Е.К. Генетическое разнообразие сортов и линий мягкой пшеницы Казахстана // В сб. Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане (под общ. ред. д.б.н. С.И. Абугалиевой, к.б.н. Е.К. Туруспекова) – Алматы, 2012. С. 67-69.
Туруспеков Е.К., Сариев Б.С., Чудинов В.А., Середа Г.А., Ортаев А.К., Тохетова Л.А., Цыганков В.И., Досжанов М., Абугалиева С.И. Генетическая и фенотипическая изменчивость коллекций ярового ячменя выращенных в различных регионах Казахстана // В сб. Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане (под общ. ред. д.б.н. С.И. Абугалиевой, к.б.н. Е.К. Туруспекова) – Алматы, 2012. С. 51-66.
Сакауова Г.Б., Ермекбаев К.А., Асатуллаев Н.Ш. О новых местонахождениях эндемичных растений в Каратау // Труды Аксу-Жабаглинского государственного заповедника. Алматы, 2016. Вып. 11. – С. 296-302.
Turuspekov Y., Kondybaev A., Volkova L., Plieske G., Ganal M., Chudinov V., Sereda G., Tokhetova L., Abugalieva S. DNA genotyping and phenotyping of spring wheat accessions from Kazakhstan // 12th International Wheat Genetics Symposium, Japan, 2013, р.107.
Abugalieva S., Quarrie S., Abugalieva A., Turuspekov Y. QTL-mapping of grain quality traits in bread wheat // 12th International Wheat Genetics Symposium, Japan, 2013, р.108.
Abugalieva S., Ermekbaev K., Sariev B., Chudinov V., Sereda G., Turuspekov Ye. Genotyping and phenotyping of oat collection // 2nd Conference of Cereal Biotechnology and Breding. – Budapest, 2013.
Turuspekov Ye., Sato K., Ebana K., Baibosynova S., Podolskih A., Abugalieva S. Genetic and agronomic characterization of rice collection from Kazakhstan // 2nd Conference of Cereal Biotechnology and Breding. – Budapest, 2013. Р.49.
Abugalieva A.I., Turuspekov E.K., Abugalieva S.I., Savin T.V. Genetic markers and biochemical evaluation in the winter triticale identification and breeding //8thInternational Triticale Symposium. June, 10th — 14th, 2013. Culture- and Convention Center Het Pand, Ghent, Belgium
Turuspekov Ye., Rsaliev S., Rsaliev A., Sato K., Abugalieva S. Identification of QTL for resistance to spot blotch caused by Bipolaris sorokiniana in Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014. P.74.
Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014. P.75.
Turuspekov Y., Plieske J., Ganal M.W., Akhunov E., Abugalieva S. SNP genotyping of wheat accessions from Kazakhstan // Book od Abstracts of EUCARPIA Cereals Section–
ITMI Joint Conference. Ulrike Lohwasser & Andreas Börner (eds.), Wernigerode 2014, P. 187.
Turuspekov Y. is a part-time lecturer (0.25 FTE) in al-Farabi Kazakh National University, where he reads lectures on «Genomics» for masters and PhD students in the Department of Biotechnology, Faculty of Biology and Biotechnology.
Abugalieva S. is a co-supervisor of studies of PhD student Zatybekov A. (KazNAU).
Turuspekov Y. is a co- supervisor of studies of PhD student Ermekbayev K. (John Innes Centre, UK, “Bolashak” program).
There are 3 master degree research works of al-Farabi Kazakh National University students under the supervison of Turuspekov Y. and Abugalieva S.
Turuspekov Y. is a member of the Dissertation Council on thesis defense in the field of biology, ecology and biotechnology at al-Farabi Kazakh National University.
The Republican State Enterprise «Institute of Plant Biology and Biotechnology», Committee of Science, Ministry of Education and Science of the Republic of Kazakhstan.
Title: «Methodical bases of plant molecular genetics»
Date: 1 – 13 of September, 2016.
Place: laboratory of molecular genetics,
The Republican State Enterprise «Institute of Plant Biology and Biotechnology», Committee of Science, Ministry of Education and Science of the Republic of Kazakhstan,
Timiryazev Street 45, Almaty 050040, Kazakhastan.
Phone: +7(727)3948006
email: absaule@yahoo.com
Cost: 100 000 tenge per person. The price does not include drive, meals and accommodation costs.
