Head of the Laboratory: Yerlan Turuspekov, Candidate of Biological Sciences, Associated professor, the h-index — 10.
The laboratory staff:
Saule Abugalieva, Leading Research Scientist, Doctor of Biological Sciences, Associated professor.
Volkova Liubov, Research Scientist, Master.
Ermekbayev Kanat, Research Scientist, PhD student, Master.
Zatybekov Alybek, Research Assistant, PhD student, Master.
Anuarbek Shynar, Research Assistant, Master.
Asatulayev Negmatullo, Research Assistant, Bachelor.
Sagyntay Fariza, Senior laboratory assistant (technician), Bachelor.
Kaparbay Raushan, Senior laboratory assistant (technician), Bachelor.
Tolenova Ayagoz, Laboratory assistant (technician), Bachelor.
Amangeldinov Kuandyk, Laboratory assistant (technician), Student of al-Farabi Kazakh National University.
Zhanarbek Serik, Laboratory assistant (technician), Student of al-Farabi Kazakh National University.
Shamshadin Daniyar, Laboratory assistant (technician), Student of al-Farabi Kazakh National University.
Genyevskaya Yuliya, Laboratory assistant (technician), Student of al-Farabi Kazakh National University.
- The major scientific directions: Genomic selection of crops in Kazakhstan, DNA-based genotyping of wild flora in Kazakhstan.
- The main results of work (within the last 3 years):
Studying the genetic diversity of domestic cultivars and promising lines of wheat, barley, rice, oat, soybean, chickpea, and pea using microsatellite DNA markers.
Genotyping of cultivars collections and lines of wheat, barley, rice and soybean based on a new genomic technology using Illumina SNP-markers.
Studying problems of wheat, barley and soybean adaptations using specific flowering-genes that control the vernalization processes and the response to photoperiod length.
Studying the genetics of the barley collection’s resistance to brown spot disease and stem rust.
Identification of DNA-markers for quantitative trait loci associated with the wheat quality.
Creation of the DNA-bank for endemic, rare and endangered species of flora in Kazakhstan.
Creation of two spring barley cultivars: Shakhristan (2013, in cooperation with Kyzylorda Research Institute of Rice breeding) and Bozman (2014, in cooperation with Karaganda Research Institute of Plant breeding and selection) together with Kazakhstan breeders.
Laboratory projects and programs (within the last 3 years):
Programs list:
“Genetic diversity and preservation of genetic resources of endemic, rare and industrially important plants in the Republic of Kazakhstan” according to «The intellectual potential of the country» priority for 2015-2017. The head is Turuspekov Y.
The list of grant financing programs, the Ministry of agriculture and the Eurasian Economic Community:
“Assessment of genetic variation and phenotypic patterns of soybean towards adaptation and high productivity in different agro-climatic conditions of Kazakhstan” according to «The grant funding for research” budget program for 2015-2017. The head is Abugalieva S.
“Development of DNA markers for wheat breeding towards improvement of grain yield and quality based on use new genomic technologies” according to «The grant funding for research» budget program for 2015-2017. The head is Turuspekov Y.
«Genetic variation and variability of agronomic traits in accessions of local and world collections of oat Avena sativa L.» according to «The grant funding for research” budget program for 2012-2014. The head is Abugalieva S.
«The study of genetic diversity and certification of commercial legumes cultivars», according to the Eurasian Economic Community’s MG.0591 «Innovative Biotechnology» interstate target program for 2012-2014. The head is Abugalieva S.
“The association mapping of genes for grain quality traits of barley” according to «The grant funding for research” budget program for 2012-2014. The head is Turuspekov Y.
“The study of the genetic diversity and search for the associations between DNA markers and agronomic traits in collection of cultivars and lines of rice from Kazakhstan” according to «The grant funding for research” budget program for 2012-2014. The head is Turuspekov Y.
“Genetic mapping of genes and quantitative trait loci associated with resistance to stem rust and spot blotch in populations of wild and cultivated barley” according to «The grant funding for research” budget program for 2013-2015. The head is Turuspekov Y.
«Molecular-genetic analysis of barley in order to create new lines and cultivars that will be resistant to stress factors and will have a high quality grain», according to the «Research in the field of agroindustrial complex and natural resources» budget program for 2012-2014. The head is Turuspekov Y.
International grant (2012-2016):
«Genetics and physiology of wheat development to flowering: tools to breed for improved adaptation and yield potential» — grant of the European Framework Programmes FP7-KBBE-2011-5, project №289842 ADAPTAWHEAT. PI from Kazakhstan is Turuspekov Y.
Scientific cooperation with international and national organizations:
Cooperation with 19 organizations from around the world, including Europe, North and South America, and Australia via the ADAPTAWHEAT international grant.
Cooperation with eight plant breeding organizations in Kazakhstan via the local projects: Karabalyk Agricultural Experimental Station, Aktobe Agricultural Experimental Station, Pavlodar Agricultural Research Institute, Karaganada Research Institute of Plant breeding and selection, Aral Experimental Station of plant genetic resources, Kazakh Research Institute of Agriculture and Plant breeding, Kazakh Research Institute of Rice breeding, Krasny Vodopad Agricultural Experimental Station.
Cooperation with 15 Kazakhstan state national parks and reserves, 5 Botanic Gardens of the Republic of Kazakhstan, National Center of Biotechnology of the Republic of Kazakhstan via «The study of genetic diversity and preservation of genetic resources of endemic, rare and economically valuable plant species in the Republic of Kazakhstan» program (ПЦФ).
Scientific-pedagogical activity:
Turuspekov Y. is a part-time lecturer (0.25 FTE) in al-Farabi Kazakh National University, where he reads lectures on «Genomics» for masters and PhD students in the Department of Biotechnology, Faculty of Biology and Biotechnology.
Abugalieva S. is a co-supervisor of studies of PhD student Zatybekov A. (KazNAU).
Turuspekov Y. is a co- supervisor of studies of PhD student Ermekbayev K. (John Innes Centre, UK, “Bolashak” program).
There are 3 master degree research works of al-Farabi Kazakh National University students under the supervison of Turuspekov Y. and Abugalieva S.
Turuspekov Y. is a member of the Dissertation Council on thesis defense in the field of biology, ecology and biotechnology at al-Farabi Kazakh National University.
International internships, honors, awards, scholarships:
2014 — Medal for contribution to the development of science in the Republic of Kazakhstan (Turuspekov Y.)
2015 — Thompson Reuters and Kazakh National Center for Scientific and Technical Information «Leader of Science» diploma (Abugalieva S. and Turuspekov Y.)
Training at the National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan (Ashimova A., 2015)
Training at the Институте растениеводства, Foggia, Italy (Anuarbek Sh., 2015)
Training at the John Innes Centre, Norwich, England (Ermekbayev K., 2015)
Training at the Okayama University and Kurashiki University, Okayama, Japan (Ermekbayev K., 2014)
Training at the John Innes Centre, Norwich, England (Kondybayev A., 2013)
Publications in 2012-2016:
Articles in peer-reviewed international journals:
- Абугалиева А.И., Абугалиева С.И., Кворри С.А., Туруспеков Е.К., Чакмак И., Савин Т.В., Ганеев В.А. Содержание Fe, Zn и S в зерне популяции дигаплоидных линий мягкой пшеницы Chinese Spring х SQ1 // Вавиловский журнал генетики и селекции. – 2012. –Т.16, №4/2. – С. 894-900. Импакт-фактор РИНЦ 0.304
- Абугалиева С.И., Середа Г.А., Чудинов В.А., Сариев Б.С., Туруспеков Е.К. Анализ хозяйственно-ценных признаков мировой коллекции овса, выращенной в трех различных регионах Казахстана // Труды по прикладной ботанике, генетике и селекции. – 2013. – Т.171. – С. 168-174. Импакт-фактор РИНЦ 0.032
- Turuspekov Y., Sariev B., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Doszhanov M., Volis S., Abugalieva S. Genotype x environment interaction patterns for grain yield of spring barley in different regions of Kazakhstan // Генетика. – 2013, том 49, № 2, с. 196-205. Impact factor 0.448
- Turuspekov Y., Abugalieva S., Ermekbayev K., Sato K. 2014. Genetic characterization of wild barley populations (Hordeum vulgare ssp. spontaneum) from Kazakhstan based on genome wide SNP analysis. Breeding Science 64:399-403. Impact factor 2.13
- Абугалиева С.И., Байбосынова С.М., Кондыбаев А.Б., Подольских А.Н., Туруспеков Е.К. Генетическое и фенотипическое разнообразие коллекции риса в Казахстане // Труды по прикладной ботанике, генетике и селекции. – 2014. – Т.175. 2014. –Т.175., вып. 4. – С. 46-59. Импакт-фактор РИНЦ 0.032
- Abugalieva A .I., TuruspekovK., Abugalieva S.I., Savin T.V. Genetic markers and biochemical evaluation in the winter triticale identification and breeding // Communications in Agricultural and Applied Biological Sciences. 2014, 79(4):233-239.
- Turuspekov Y., Plieske J., Ganal M., Akhunov E., Abugalieva S. Phylogenetic analysis of wheat cultivars in Kazakhstan based on the wheat 90K single nucleotide polymorphism array. Plant Genetic Resources, 2015, available on CJO2015. doi:10.1017/S1479262115000325. Impact factor 0.442
- Volis S., Ormanbekova D., Yermekbayev, SongM.,Shulgina I. The Conservation Value of Peripheral Populations and a Relationship Between Quantitative Trait and Molecular Variation // Evolutionary Biology. – 2015. – Vol. 42, N4. – P.1-11. Impact factor 2.267
- Volis S., Ormanbekova D., Yermekbayev K. Role of phenotypic plasticity and population differentiation in adaptation to novel environmental conditions // Ecology and Evolution. – Vol.5, N17. –P. 3818-3829. Impact factor 2.537
- Volis S., Ormanbekova D., Yermekbayev K., Song M., Shulgina I. Multi-Approaches Analysis Reveals Local Adaptation in the Emmer Wheat (Triticum dicoccoides) at Macro- but not Micro-Geographical Scale // PLoS ONE. – 2015. – Vol.10, N3. – P. e0121153. Impact factor 3.234.
- Turuspekov Y., Ormanbekova D., Rsaliev A., Abugalieva S. Genome-wide association study on stem rust resistance in Kazakh spring barley lines // BMS Plant Biology. 2016. Vol. 16. N.1. P.13-21. DOI 10.1186/s12870-015-0686-z Impact factor 3.813
- Volis S., Ormanbekova D., Yermekbayev K., Abugalieva S., Turuspekov Y., Shulgina I. Genetic architecture of adaptation to novel environmental conditions in a predominantly selfing allopolyploid plant // Heredity. 2016. DOI: 10.1038/hdy.2016.2 Impact factor 3.801
Articles in journals included in the list of Education and Science Monitoring Committee, Ministry of Education and Science of the Republic of Kazakhstan.
Абугалиева С.И., Волкова Л.А., Ермекбаев К.А., Туруспеков Е.К. Генотипирование коммерческих сортов яровой мягкой пшеницы Казахстана с использованием микросателлитных ДНК-маркеров // Биотехнология. Теория и практика. 2012. №2. – С.35-45. DOI: http://dx.doi.org/10.11134/btp.2.2012.4
- Абугалиева С.И., Волкова Л.А., Нурланова А.А., Жанпеисова А.С., Туруспеков Е.К. ДНК-фингерпринтинг сортов сои Казахстана с использованием микросателлитных маркеров // Биотехнология. Теория и практика. – 2013. — №3. – С. 26-34. http://dx.doi.org/10.11134/btp.3.2013.4
- Абугалиева С.И. Генетическое разнообразие сои (Glycine max (L.) Merrill) // Биотехнология. Теория и практика. — 2013. — №4. – С. 13-19. http://dx.doi.org/10.11134/btp.4.2013.2
- Волкова Л.А., Абугалиева С.И., Туруспеков Е.К. Вариабельность нуклеотидных и аминокислотных последовательностей хордоиндолиновых генов в коллекции ярового ячменя Казахстана // Биотехнология. Теория и практика. – 2014. — №2. – С. 48-58.
- Туруспеков Е.К., Рсалиев Ш.С., Рсалиев А.С., Ермекбаев К.А., Абугалиева С.И. Поиск ДНК-маркеров устойчивости ячменя к стеблевой ржавчине // Вестник КазНУ, Серия биологическая. – 2015. – №3(65). – С.162-169. Импакт-фактор АО НЦ НТИ 0,041
- Әнуарбек Ш.Н., Волкова Л.А., Абугалиева С.И., Туруспеков Е.К. Скрининг мировой коллекции сои с использованием ДНК-маркеров // Вестник КазНУ, Серия биологическая. – 2015. – №3(65). – С.110-117. Импакт-фактор АО НЦ НТИ 0,041
Articles in the digest of scientific works and conferences in the Republic of Kazakhstan:
- Абугалиева С.И., Ермекбаев К.А., Волкова Л.А., Орманбекова Д.Е., Туруспеков Е.К. Генетическое разнообразие сортов и линий мягкой пшеницы Казахстана // В сб. Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане (под общ. ред. д.б.н. С.И. Абугалиевой, к.б.н. Е.К. Туруспекова) – Алматы, 2012. С. 67-69.
- Туруспеков Е.К., Сариев Б.С., Чудинов В.А., Середа Г.А., Ортаев А.К., Тохетова Л.А., Цыганков В.И., Досжанов М., Абугалиева С.И. Генетическая и фенотипическая изменчивость коллекций ярового ячменя выращенных в различных регионах Казахстана // В сб. Современное состояние и перспективы развития генетики и селекции зерновых культур в Казахстане (под общ. ред. д.б.н. С.И. Абугалиевой, к.б.н. Е.К. Туруспекова) – Алматы, 2012. С. 51-66.
- Сакауова Г.Б., Ермекбаев К.А., Асатуллаев Н.Ш. О новых местонахождениях эндемичных растений в Каратау // Труды Аксу-Жабаглинского государственного заповедника. Алматы, 2016. Вып. 11. – С. 296-302.
Request for a patent:
- Кожахметов К., Абугалиева С.И., Туруспеков Е.К. Пшеница мягкая озимая ГЛИКК // Промышленная собственность. Официальный бюллетень. –2015. №5. –С.136-137.
Monographs and scientific guidelines:
- Абугалиева С.И., Туруспеков Е.К. «Изучение генетического разнообразия и паспортизация сортов зернобобовых культур». Методические рекомендации. ISBN 978-601-7343-05-7. Алматы, 2014. 56 с.
Theses
In far abroad countries:
- Turuspekov Y., Kondybaev A., Volkova L., Plieske G., Ganal M., Chudinov V., Sereda G., Tokhetova L., Abugalieva S. DNA genotyping and phenotyping of spring wheat accessions from Kazakhstan // 12th International Wheat Genetics Symposium, Japan, 2013, р.107.
- Abugalieva S., Quarrie S., Abugalieva A., Turuspekov Y. QTL-mapping of grain quality traits in bread wheat // 12th International Wheat Genetics Symposium, Japan, 2013, р.108.
- Abugalieva S., Ermekbaev K., Sariev B., Chudinov V., Sereda G., Turuspekov Ye. Genotyping and phenotyping of oat collection // 2nd Conference of Cereal Biotechnology and Breding. – Budapest, 2013.
- Turuspekov Ye., Sato K., Ebana K., Baibosynova S., Podolskih A., Abugalieva S. Genetic and agronomic characterization of rice collection from Kazakhstan // 2nd Conference of Cereal Biotechnology and Breding. – Budapest, 2013. Р.49.
- Abugalieva A.I., Turuspekov E.K., Abugalieva S.I., Savin T.V. Genetic markers and biochemical evaluation in the winter triticale identification and breeding //8thInternational Triticale Symposium. June, 10th — 14th, 2013. Culture- and Convention Center Het Pand, Ghent, Belgium
- Turuspekov Ye., Rsaliev S., Rsaliev A., Sato K., Abugalieva S. Identification of QTL for resistance to spot blotch caused by Bipolaris sorokiniana in Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014. P.74.
- Turuspekov Ye., Rsaliev S., Rsaliev A., Tokhetova L., Chudinov V., Sereda G., Ortaev A., Sariev B., Abugalieva S. Resistance to stem rust in spring barley from Kazakhstan // 1st International Workshop on Barley Leaf Diseases, Salsomaggiore Terme, 03-06 June 2014. P.75.
- Turuspekov Y., Plieske J., Ganal M.W., Akhunov E., Abugalieva S. SNP genotyping of wheat accessions from Kazakhstan // Book od Abstracts of EUCARPIA Cereals Section–
ITMI Joint Conference. Ulrike Lohwasser & Andreas Börner (eds.), Wernigerode 2014, P. 187.
- Turuspekov Y., Sariev B., Abugalieva A., Chudinov V., Sereda G., Tokhetova L., Ortaev A., Tsygankov V., Yespanov A., Blake T., Sato K., Abugalieva Comparative genetic and phenotypic variation of USA and Kazakhstan barley accessions // Book od Abstracts of EUCARPIA Cereals Section – ITMI Joint Conference. Ulrike Lohwasser & Andreas Börner (eds.), Wernigerode 2014, P. 188.
- Turuspekov Y., Abugalieva S. Plant DNA barcoding project in Kazakhstan // Genome. –2015. -Vol. 58, N.5. – P.290.
- Turuspekov Ye., Chudinov V., Sereda G., Tokhetova L., Griffits S., Plieske J., Ganal M., Akhunov E., Abugalieva S. Phenotypic and genotypic variability of wheat in Kazakhstan // 9th International Wheat Conference. Abstracts book. — P.62.
- Abugalieva S., Didorenko S., Ormanbekova D., Anuarbek S., Turuspekov Y. GWAS on Flowering Time in Collection of Soybean Grown in Kazakhstan // Abstracts of International Conference Plant Genetics and Breeding Technologies II. Vienna, Austria. 2016. P. 35.
In the near abroad:
- Волкова Л.А., Ермекбаев К.А., Абугалиева С.И., Туруспеков Е.К. Генетическое разнообразие сортов сои Казахстана // «Селекция и генетика сельскохозяйственных растений: традиции и перспективы»: тез. докл. межд. науч. конф., посв. 100-летию Селекционно-генетического института – Национального центра семеноведения и сортоизучения. – Одесса, 2012. – С.138-139.
- Абугалиева С.И., Сариев Б.С., Чудинов В.А., Середа Г.А., Ортаев А.К., Тохетова Л.А., Цыганков В.И., Ермекбаев К.А., Туруспеков Е.К. Молекулярно-генетический и морфологический анализ сортов и линий ячменя // «Селекция и генетика сельскохозяйственных растений: традиции и перспективы»: тез. докл. межд. науч. конф., посв. 100-летию Селекционно-генетического института – Национального центра семеноведения и сортоизучения. – Одесса, 2012. – С.119-120.
- Туруспеков Е.К., Тохетова Л.А., Сариев Б.С., Чудинов В.А., Середа Г.А., Ортаев А.К., Цыганков В.И., Абугалиева А.И., Рсалиев Ш.С., Еспанов А., Блейк Т., Сато К., Абугалиева С.И. Комплексный генетико-селекционный анализ коллекционных образцов ярового ячменя в Казахстане // Материалы международной конференции «Генетические ресурсы растений – основа продовольственной безопасности и повышения качества жизни», г. Санкт-Петербург, Россия, 6-8 октября 2014 г. С.94.
- Абугалиева С.И., Байбосынова С.М., Кондыбаев А.Б., Подольских А.Н., Сато К., Туруспеков Е.К. Генетическое и фенотипическое разнообразие коллекции риса в Казахстане // Материалы международной конференции «Генетические ресурсы растений – основа продовольственной безопасности и повышения качества жизни», г. Санкт-Петербург, Россия, 6-8 октября 2014 г. С.40.
- Туруспеков Е.К., Плиске Й., Ганал М., Ахунов Э., Абугалиева С.И. Филогенетический анализ коллекций культурной пшеницы с использованием чипа для 90 тысяч SNP-маркеров // Материалы 2-ой международной конференции «Проблемы эволюции и систематики культурных растений». г. Санкт-Петербург, Россия, 9-10 октября 2014 г. С.46.
- Абугалиева С. И., Чудинов В.А., Середа Г.А., Сариев Б.С., Туруспеков Е.К. Генетическое и фенотипическое разнообразие коллекции овса (Avena sativa) // Материалы 2-ой международной конференции «Проблемы эволюции и систематики культурных растений». г. Санкт-Петербург, Россия, 9-10 октября 2014 г. С.36.
- Turuspekov Y., Rsaliev A., Chudinov V., Sarbayev A.. Abugalieva S. Identification of QTLs associated with spot blotch resistance of barley // The 3d International Conference “Plant Genetics, Genomics, Bioinformatics and Biotechnology” PlantGen 2015 Abstracts book. Новосибирск, ИЦиГ СО РАН. – С.59-60.
- Turuspekov Y., Plieske J., Ganal M., Akhunov E., Abugalieva S. Genetic variation of spring wheat in Kazakhstan // The 3d International Conference “Plant Genetics, Genomics, Bioinformatics and Biotechnology” PlantGen 2015 Abstracts book. Новосибирск, ИЦиГ СО РАН. – С.59.
In the Republic of Kazakhstan:
- Қапарбай Р.Е. Іле-Алатауы ұлттық паркіндегі ақезу бәрпісі (Aconitum leucostomum ) өсімдігінің жылдықдамуы // Фараби әлемі: тез. докл. междунар. науч. конф. студентов и молодых ученых. – Алматы: Қазақ университеті, 2015. – С. 90.
- Төленова А.Д. Сравнительный анализ состояния интродукционных и природных популяций девясила высокого (Inula helenium L.) в Заилийском Алатау // Фараби әлемі: тез. докл. междунар. науч. конф. студентов и молодых ученых. – Алматы: Қазақ университеті, 2015. – С. 127.
- Әнуарбек Ш.Н. Изучение генетического разнообразия коллекции гороха с использованием SSR-маркеров // Фараби әлемі: тез. докл. междунар. науч. конф. студентов и молодых ученых. – Алматы: Қазақ университеті, 2015. – С. 140.
Laboratory services:
School of Young Scientists in the laboratory of molecular genetics
The Republican State Enterprise «Institute of Plant Biology and Biotechnology», Committee of Science, Ministry of Education and Science of the Republic of Kazakhstan.
Title: «Methodical bases of plant molecular genetics»
Date: 1 – 13 of September, 2016.
Place: laboratory of molecular genetics,
The Republican State Enterprise «Institute of Plant Biology and Biotechnology», Committee of Science, Ministry of Education and Science of the Republic of Kazakhstan,
Timiryazev Street 45, Almaty 050040, Kazakhastan.
Phone: +7(727)3948006
email: absaule@yahoo.com
Cost: 100 000 tenge per person. The price does not include drive, meals and accommodation costs.
The program of theoretical and practical classes:
Introduction to the problem. Safety instructions. Work process in the lab. Issuance of the course materials.
Day 1. Methods of express DNA extraction for PCR. Preparation of solutions. Disruption of cells and tissues, removing of proteins and impurities. DNA Precipitation.
Day 2. Checking the quality and quantity of DNA. Agarose gel electrophoresis. Spectrophotometer.
Day 3. PCR theory. Formulation of the problem. Ingredients of the reaction. PCR running.
Day 4. Gels filling. Electrophoresis. Gel — documentation system. Documenting of results. Cutting off of DNA-bands from agarose gels.
Day 5. Using of the commercial kits for DNA purification.
Day 6. PCR for DNA sequencing. Purification of PCR products from the excess of dye, primers and other impurities.
Day 7. Introduction to the DNA sequencing machine (DNA sequencer). Start of DNA sequencing.
Day 8. Documentation of the sequencing results. Comparative analysis of nucleotide sequences.
Day 9. Work with application software for cluster analysis of samples. An analysis of massive data for samples genotyping. Results interpretation.
Day 10. Discussion of results . Issuance of certificates.
Commercial Products: None