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AP 09260382 (superviser Sapakhova Z.)

Brief information about the project

Name of the project: IRN AP09260382 «Production of sweetpotato plants tolerant to abiotic stresses and determination methods for its effective cultivation».

Sweetpotato (Ipomoea batatas) grown in over 100 countries around the world. Sweetpotato is considered as a food security crop and staple food in the rural economies of many countries.

Sweetpotato is a heat-loving plant, it takes about 90 warm days to form a tuber. The optimal temperature for growth and development is 24°C. For large-scale cultivation and cost-effective production, even in the southern regions of Kazakhstan, cold tolerant cultivars are required. Nowadays, this agricultural crop among the population in Kazakhstan is practically unknown and is not cultivated on an industrial scale, therefore it is very important to optimize agricultural technology for effective cultivation.

In Kazakhstan, sweetpotato have been studied for the last 5 years at the National Center for Biotechnology of the Republic of Kazakhstan and by the authors of the project (IPBB). Studies have shown that in the southeast of Kazakhstan, this crop gives a good harvest results, but in years with spring frosts, the harvest results is decreasing.

One of the most effective ways to increase plant tolerance simultaneously to several abiotic stress factors is the use of genetic engineering methods to increase the rate of proline biosynthesis and reduce the rate of its biodegradation in plant. Proline is the second osmolytic after carbohydrates in terms of prevalence and content in plant, and there is a positive correlation between the level of its content in plant tissues and their tolerance to abiotic stress factors.

To achieve the goal of the project, namely, obtaining cold-resistant sweetpotato lines, we intend to increase the collection of sweetpotato, select the best varieties in terms of yield and biochemical composition, on their basis, create cisgenic plants resistant to abiotic stresses by suppressing the proline dehydrogenase gene.

Project goal: To select the best sweetpotato cultivars in terms of yield and biochemical composition of tubers and, on their basis, create cisgenic plants resistant to abiotic stress by suppressing the proline dehydrogenase gene and develop scientifically based agrotechnics for the effective cultivation of sweetpotato in the Almaty region of Kazakhstan.

Expected results:

The collection of sweetpotato will be expanded (no less than 35 samples) for field trials and selection of the best varieties for genetic transformation

Genetic construction for suppressing the proline dehydrogenase gene

Scientifically grounded agrotechnology for growing sweetpotato in the Almaty region of Kazakhstan. (Optimization of the timing and density of planting seedlings, covering the ridges with various films and mulch).

The selection of the best collection samples of sweet potatoes in terms of yield and biochemical composition (vitamins, sugar, starch) will be carried out based on the results of growing the first year of field trials

Sweetpotato plants will be obtained carrying a DNA region in the genome encoding dsRNA to the conserved region of the ProDH gene.

Sweet potato plants carrying a DNA region encoding dsRNA to the conserved region of the proline dehydrogenase (ProDH) gene for resistance to cold and drought will be studied

The main hypotheses of the project will be confirmed or refuted.

Project manager:

Sapakhova Zagipa Beisenovna, higher education (specialty Biology), PhD, scientific work experience of 17 years corresponds to the profile of the scientific project, h-index-3. https://www.scopus.com/authid/detail.uri?authorId=56046815800. Author ID in Scopus — 56046815800, Researcher ID Web of Science — M-6615-2015, ORCID ID — 0000-0002-8007-5066, Research Gate: Zagipa Sapakhova.

 

 

Member in the research group:

Zhapar Kuanysh Kabyluly: bachelor (specialty Biology), master (specialty Biology), doctoral student of KazNAU (specialty Plant Protection and Quarantine), scientific work experience of 7 years corresponds to the profile of the scientific project, h-index 1. Scopus Author ID – 57201671071. ORCID ID — 0000-0002-9007-9730.     (https://www.scopus.com/authid/detail.uri?authorId=57201671071; https://app.webofknowledge.com/author/record/13315794).

Zhigalov Andrey Viktorovich: higher education (specialty biology), candidate of biological sciences (specialty molecular biology), 15 years of scientific work experience corresponds to the profile of the scientific project, h-index 2.      (https://www.scopus.com/authid/detail.uri?origin=resultslist&authorId=6508121286&zone=&featureToggles=FEATURE_AUTHOR_DETAILS_BOTOX:1&at_feature_toggle=1), (https://app.webofknowledge.com/author/record/4848976?lang=ru_RU&SID=E6Z3oWNmLijDjWIAkQB).

Shamekova Malika Khabidulayevna: higher education (specialty Zootechnic), Master’s degree (specialty Agroecology), PhD (specialty Ecology), currently Head of the Laboratory of Breeding and Biotechnology of the Institute of Plant Biology and Biotechnology, associate professor, 15 years of research experience corresponds to the profile of the scientific project, h-index 4, 1 PhD thesis was defended under her scientific supervision.

https://www.scopus.com/authid/detail.uri?authorId=55617198500

https://app.webofknowledge.com/author/record/4172091

Daurov Dias Lamzarovich: higher education (specialty Biotechnology), PhD doctoral student (specialty Ecology), 8 years of research experience corresponds to the profile of the scientific project, h-index-1.  https://www.scopus.com/authid/detail.uri?origin=resultslist&authorId=57201666405&zone=. Author ID в Scopus – 57201666405, Researcher ID Web of Science – AAP-5148-2020, ORCID ID – 0000-0003-3073-4577, Research Gate: Dias Daurov.  (https://www.scopus.com/authid/detail.uri?origin=resultslist&authorId=57201666405&zone=; https://app.webofknowledge.com/author/record/35819805?lang=ru_RU&SID=F5UIG1R9xCKg1bvOMMo).

Bakbergenova Makpal Oryntaevna: higher education (specialty Agronomy), 6 years of scientific work experience corresponds to the profile of the scientific project, h-index-1. https://www.scopus.com/authid/detail.uri?authorId=57201670063. Author ID в Scopus – 57201670063.

Kairkanov Bolat Kozhekbaevich: general secondary education.

Zhizhin Nikolay Ivanovich: higher education (specialty Chemistry), 15 years of scientific work experience and 4 of them correspond to the profile of the scientific project.

List of publications and patents of the project performers for the period from 2015 to 2020.

  1. Madenova A., Sapakhova Z., Bakirov S., Galymbek K., Yernazarova G., Kokhmetova A., Keishilov Z. Screening of wheat genotypes for the presence of common bunt resistance genes // Saudi Journal of Biological Sciences. 2021. Vol. 28(5). P. 2816-2823. org/10.1016/j.sjbs.2021.02.013
  2. Madenova A., Kokhmetova A., Sapakhova Z., Galymbek K., Keishilov Z., Akan K., Yesserkenov A. Effect of common bunt (Tilletia caries (DC) Tul) infection on agronomic traits and resistance of wheat entries // Research on Crops. 2020. 21 (4). P. 791-797. DOI : 10.31830/2348-7542.2020.121
  3. Kokhmetova A., Ali Sh., Sapakhova Z., Atishova M. Identification of genotypes-carriers of resistance to tan spot Ptr ToxA and Ptr ToxB of Pyrenophora tritici-repentis in common wheat collection // Vavilov Journal of Genetics and Breeding – 2018. – 22 (8). – P. 978-986. DOI 10.18699/VJ18.440.
  4. Kokhmetova A., Kremneva O., Volkova G., Atishova M., Sapakhova Z. Evaluation of wheat cultivars growing in Kazakhstan and Russia for resistance to tan spot // Journal of Plant Pathology. – 2017. – Vol. 99 (1). – P. 161-167. DOI: 10.4454/jpp. v99i1.3812.
  5. Morgounov A., Akin B., Demir L.A., Keser M., Kokhmetova A., Martynov S., Orhan A., Ozdemir F., Azseven A., Yessimbekova M., Sapakhova Z. Yield gain in leaf rust resistant and susceptible winter wheat varieties due to fungicide application // Crop and Pasture Science. – – 66 (7). P. – 649-659. http://dx.doi.org/10.1071/CP14158.
  6. Zhigailov,V., Alexandrova,A.M., Nizkorodova,A.S., Stanbekova.G.E., KryldakovR.V., Karpova O.V., PolimbetovaN.S., Halford,N.G., Iskakov,B.K. Evidence That Phosphorylation of the α-Subunit of eIF2 Does Not Essentially Inhibit mRNA Translation in Wheat Germ Cell-Free System. Frontiers in Plant Science, 2020, 11, 936     DOI:1010.3389/fpls.2020.00936  IF – 4,4,  Q1, P (Scopus) – 95.
  7. Nizkorodova, A., Suvorova, M., Zhigailov, A., Iskakov, B. The Effect of Translation Promoting Site (TPS) on Protein Expression in E. coli Cells. Molecular Biotechnology. Volume 62, Issue 6-7, 1 July 2020, Pages 326-334 IF – 2, Q3, P (Scopus) – 54.
  8. Temirbayeva K., Zhang M.L., Molecular phylogenetic and biogeographical analysis of Nitraria based on nuclear and chloroplast DNA sequences. Plant Systematics and Evolution. 2015. 301 (7): 1897-1906. DOI: 10.1007/s00606-015-1202-5. IF(WoS)-1.3 (2019), Q3, P (Scopus) – 73.
  9. Zhang M.L., Temirbayeva K., Saсnderson S.C., Chen X. Young dispersal of xerophil Nitraria lineages in intercontinental disjunctions of the Old World. Scientific Reports. 2015. DOI: 10.1038/srep13840. IF(WoS)- 3.99, Q1, P (Scopus) – 93.
  10. Zhambakin, K., Zhapar, K. Current status and prospects of plant biotechnology in Kazakhstan. Plant Biotechnology Report  14, 177–184 (2020). DOI:10.1007/s11816-020-00601-0  IF-1.259, Q-3, P (Scopus) – 64.
  11. Daurov D., Zhapar K., Daurova A., Volkov D., Bakbergenova M., Tolegenova D., Shamekova M., Zhambakin K. Production of virus-free sweet potato planting material for the southeast of Kazakhstan. International journal of agriculture and biology 20 (4) : 851-856, 2018. DOI: 17957/IJAB/15.0576.      IF (WoS)  – 0,82, Q-3, P (Scopus) – 56.
  12. Daurova A., Daurov D., Volkov D., Karimov A., Zhandos A., Raimbek D., Zhapar K., Zhambakin K., Shamekova M.  Mutagenic treatment of microspore-derived embryos of turnip rape (Brassica rapa) to increase oleic acid content. Plant Breeding. 2020; 00:1–7. DOI:10.1111/pbr.12830 IF (WoS) – 1.251, Q -3, P (Scopus) – 62.
  13. Daurov, D., Daurova, A., Karimov, A. Tolegenova, D., Volkov, D., Raimbek D., Zhambakin K.,Shamekova M. Determining Effective Methods of Obtaining Virus-Free Potato for Cultivation in Kazakhstan. Am.J.PotatoRes. 97, 367–375 (2020).  DOI:10.1007/s12230-020-09787-z, IF (WoS) -1.085, Q-3, P (Scopus) – 57.
  14. Daurova A, Daurov D, Volkov D, Zhapar K, Raimbek D, Shamekova M, Zhambakin K. 2020. Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits. OCL 27: 45. DOI:1051/ocl/2020041; SiteCoreScopus-2.8, P (Scopus) – 70
  15. Daurov D, Zhapar K, Daurova A, Volkov D, Borhan I, Zhambakin K, Shamekova M. Isolated meristem culture of sweet potatoes. Research, results 2: 34-42, 2017.
  16. Zhapar K, Daurov D, Zhambakin K, Shamekova M. Sweet potato’s resistance to various stress factors. Science News of Kazakhstan 2(132) : 90 -112, 2017.
  17. Daurova A, Daurov D, Zhapar K, Volkov D, Zhambakin K, Shamekova M. Production of transgenic sweet potato plants with the dreb1a gene. News of the National Academy of Sciences of the Republic of Kazakhstan, Biological and medical series. 2 (309) : 71-77, 2017.

Results obtained for 2021

The collection of sweet potatoes was expanded to 35 varieties from various sources such as local farms, as well as from near and far abroad (South Korea, Australia, Ukraine, Russia). Computer analysis of sweet potato genes was carried out: IbRbcS1, GBSS-I, primers and DNA fragments were synthesized for cloning a promoter, terminator and cis-gene insert for RNA silencing. The promoter of the sweet potato ribulose-1,5-bisphosphate carboxylase small subunit gene (IbRbcS1) was cloned. The transcription terminator of the starch synthase I gene associated with sweet potato granules (GBSS-I) was cloned. The DNA region encoding dsRNA for the conserved region of the sweet potato proline dehydrogenase (ProDH) gene was cloned under the control of the sweet potato cisgenic regulators (promoter and transcription terminator). The optimal timing of planting seedlings (early June), the optimal planting density of seedlings (30/80 cm) were determined.

Publication: Published 1 article in a journal included in the CCSES list:

Zhapar K., Daurov D., Volkov D., Daurova A., Tolegenova D., Abay Z., Argynbaeva A., Kim H.S., Kwak S.S., ShamekovaM., Zhambakin K. Selection of sweetpotato cultivars with high yields in Almaty region, Kazakhstan // Al-Farabi Kazakh National University. Experimental Biology. — 2021. — №3 (88). — Р. 45-52.