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HomeAP 14869593 (superviser Almerekova Sh.)

AP 14869593 (superviser Almerekova Sh.)

Brief description of the project

(2022-2024)

 

Project title: IRN AP14869593 «Study of genetic diversity and phytochemical analysis of species of the genus Salsola L. in Kazakhstan».

Relevance. Approximately 60% of the territory of Kazakhstan is occupied by deserts and semi-deserts with peculiar vegetation, where Salsola species (saltwort) are the dominant component of plants community. Also, saltworts are an important source of medicinal raw materials, valuable food resources at arid territories, bioremediants and sand fixers. Therefore, a comprehensive study of Salsola species for the conservation and rational usage of genetic resources using the methods of botany, molecular genetics, new generation technologies and phytochemistry is highly important from scientific and practical points of view. These comprehensive study of valuable Kazakhstan’s Salsola species has never been done before. In addition, there is no generally accepted taxonomy of the genus Salsola and questions of the molecular taxonomy are unresolved. The use of next generation sequencing (NGS) technology in the project will contribute to resolving the taxonomic issues of the genus.

The goal of the project: study of genetic diversity and phytochemistry of Salsola L. species of Kazakhstan using modern molecular genetics and biochemical methods and new generation genomic technologies.

Expected results:

  1. The collection of populations of Salsola species will be carried out, the species identity will be determined, herbariums will be designed and digitized, phytocenoses with the participation of Salsola species will be described.
  2. Phylogenetic relationships among Salsola species will be studied using markers of nuclear and chloroplast genomes and DNA barcoding technology.
  3. Phylogenetic relationships among Salsola species will be analyzed using chloroplast genome sequencing.
  4. Macro- and microelement composition and antioxidant activity of Kazakhstan’s Salsola species will be studied.

Scientific Supervisor of the project: Almerekova Shyryn, senior researcher, PhD (2018, geobotany). Co-author of 35 scientific papers, including 21 articles, including 12 articles in peer-reviewed publications indexed in Web of Science (H-index=4, https://www.webofscience.com/wos/author/record/9617158) and/or Scopus (H-index=4, https://www2.scopus.com/authid/detail.uri?authorId=57196952712), and 9 articles in journals recommended by the Committee for Control in the Sphere of Education and Science (CCSES) Ministry of Education and Science of the Republic of Kazakhstan, 1 study guide, 1 copyright certificate, 1 patent of the Republic of Kazakhstan for a utility model. Winner of a scholarship for talented young scientists of the Republic of Kazakhstan (2021).

 

Research group: 

Abugalieva S., principal researcher, Doctor of Biological Sciences, Professor. Co-author of >90 articles (including 39 articles in Web of Science databases (H-index=8, https://www.webofscience.com/wos/woscc/citation-report/b75075f1-aa93-49e7-b3c5-86e58315e8a1-2f7a75ee) and/or Scopus (H-index-8, https://www2.scopus.com/authid/detail.uri?authorId=6507029042); 47 articles in CCSES et al.

Zatybekov A., PhD, senior researcher, co-author of 23 scientific articles: 7 in peer-reviewed journals Web of Science and Scopus (H-index–3, https://www2.scopus.com/authid/detail.uri?authorId =57196942983), 16 in publications of CCSES; co-author of 1 scientific and methodological recommendation, 2 utility model patents.

Veselova P., Candidate of Biological Sciences (botany), Leading Researcher of the Laboratory of Higher Plant Flora of the Institute of Botany and Phytointroduction (IBP) Committee of Forestry and Wildlife, Ministry of Ecology and Natural Resources of the RK. Has >100 publications, H-index=3 (https://www2.scopus.com/authid/detail.uri?authorId=57193084177, H-index=3 by WoS, https://www.webofscience.com/wos /author/record/41761960).

Aimenova Z., PhD, head of the research laboratory «Phytochemistry» at the Research Institute of Natural and Technical Sciences of the Auezov SKU. Author of >40 scientific papers, including 6 articles in scientific journals, CCSES, 3 articles indexed in Scopus (H-index=1, https://www2.scopus.com/authid/detail.uri?authorId=57074125500), 1 patent for invention, 10 manuals, 1 monograph.

Yermagambetova M., MS (Geobotany), junior researcher, has 4 years of experience.

Genievskaya Y., MS (Biotechnology), junior researcher, co-author of 11 articles indexed in Web of Science and/or Scopus (H-index=5, https://www2.scopus.com/authid/detail.uri?authorId =57196939730).

Osmonali B., junior researcher at the Laboratory of Higher Plant Flora of the IBP, PhD- student (Geobotany) of al-Farabi Kazakh National University on the topic of the project. Co-author of >15 publications, incl. 5 scientific articles indexed in the Web of Science and/or Scopus databases (H-index=2, https://www2.scopus.com/authid/detail.uri?authorId=57212564484).

 

List of publications of the project’s participants (2017-2022)

  1. Almerekova, S., Mukhitdinov, N., Abugalieva, S. (2017). Phylogenetic study of the endemic species Oxytropis almaatensis (Fabaceae) based on nuclear ribosomal DNA ITS sequences. BMC plant biology (IF= 4.96, quartile-Q1; SJR=1.378, percentile-87), 17(1), 1-9. DOI: 1186/s12870-017-1128-x;
  2. Almerekova, S., Shchegoleva, N., Abugalieva, S., Turuspekov, Y. (2020). The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae). PloS one (IF=3.788, quartile-Q2; SJR=0.990, percentile-92), 15(10), e0240121. DOI:1371/journal.pone.0240121
  3. Özek, G.; Schepetkin, I.A.; Yermagambetova, M.; Özek, T.; Kirpotina, L.N.; Almerekova, S.S.; Abugalieva, S.I.; Khlebnikov, A.I.; Quinn, M.T. (2021) Innate Immunomodulatory Activity of Cedrol, a Component of Essential Oils Isolated from Juniperus Species. Molecules (IF=4.588, quartile-Q2; SJR=0.990, percentile-74), 26, 7644.
    DOI:3390/molecules26247644
    Almerekova, S., Lisztes-Szabó, Z., Mukhitdinov, N., Kurmanbayeva, M., Abidkulova, K., Sramko, G. (2018). Genetic diversity and population genetic structure of the endangered Kazakh endemic Oxytropis almaatensis (Fabaceae). Acta Botanica Hungarica (SJR=0.417, percentile-50) 60(3-4), 263-278. DOI: 10.1556/034.2018.1
  4. Almerekova, S., Favarisova, N., Turuspekov, Y., Abugalieva, S.*(2020). Cross-Genera Transferability of Microsatellite Markers and Phylogenetic Assessment of Three Salsola Species from Western Kazakhstan. Proceedings of the Latvian Academy of Sciences. Section B: Natural, Exact, and Applied Sciences (Latvia) (SJR=0.168, percentile-39), 74 (5), 325-334. DOI:2478/prolas-2020-0049.
  5. Almerekova, S., Abugalieva, S*, Mukhitdinov, N. (2018). Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan. Vavilovskii Zhurnal Genetiki i Selektsii= Vavilov Journal of Genetics and Breeding. (SJR=0.188, percentile-38), 22(2), 285-290. DOI:10.18699/VJ18.362
  6. Abugalieva, S., Volkova, L., Genievskaya, Y., Ivaschenko, A., Kotukhov, Y., Sakauova, G., Turuspekov, Y. (2017). Taxonomic assessment of Allium species from Kazakhstan based on ITS and matK markers. BMC plant biology (IF=4.96, quartile-Q1; SJR=1.378, percentile-87), 17(2), 51-60. DOI:10.1186/s12870-017-1194-0
  7. Genievskaya, Y., Abugalieva, S., Zhubanysheva, A., Turuspekov, Y. (2017). Morphological description and DNA barcoding study of sand rice (Agriophyllum squarrosum, Chenopodiaceae) collected in Kazakhstan. BMC Plant Biology (IF=4.96, quartile-Q1; SJR=1.378, percentile — 87), 17(1), 1-8. DOI:1186/s12870-017-1132-1
  8. Sumbembayev, A., Abugalieva, S., Danilova, A., Matveyeva, E., & Szlachetko, D. (2021). Flower morphometry of members of the genus Dactylorhiza Necker ex Nevski (Orchidaceae) from the Altai Mountains of Kazakhstan. Biodiversitas Journal of Biological Diversity (SJR=0.257, percentile-40), 22(8). DOI:13057/biodiv/d220855
  9. Turuspekov, Y., Genievskaya, Y., Baibulatova, A., Zatybekov, A., Kotuhov, Y., Ishmuratova, M., Imanbayeva A., Abugalieva, S. (2018). Phylogenetic taxonomy of Artemisia species from Kazakhstan based on matK analyses. Proceedings of the Latvian Academy of Sciences. Section B: Natural, Exact, and Applied Sciences (Latvia) (SJR=0.168, percentile-39), 72(1), 29. DOI:10.1515/prolas-2017-0068.
  • Turuspekov, Y., Abugalieva, S., Ermekbayev, K., Sato, K. (2014). Genetic characterization of wild barley populations (Hordeum vulgare spontaneum) from Kazakhstan based on genome wide SNP analysis. Breeding Science, (IF=2.63, quartile–Q2; SJR=0.799, percentile-74) 64(4), 399-403. DOI:10.1270/jsbbs.64.399
  1. Friesen, N., Vesselova, P., Osmonaly, B., Sitpayeva, G., Luferov, A., & Shmakov, A. (2021). Allium toksanbaicum (Amaryllidaceae), a new species from Southeast Kazakhstan. Phytotaxa (IF=1.198, quartile–Q3; SJR=0.443, percentile-50), 494(3), 251-267.DOI:10.11646/phytotaxa.494.3.1
  2. Seidl, A., Tremetsberger, K., Pfanzelt, S., Blattner, F. R., Neuffer, B., Friesen, N., Hurka, H., Shmakov A., Batlai O., Žerdoner Čalasan, A., Vesselova, P., Bernhardt, K-G. (2021). The phylogeographic history of Krascheninnikovia reflects the development of dry steppes and semi-deserts in Eurasia. Scientific reports (IF=5.134, quartile–Q1; SJR=1.240, percentile-93), 11(1), 1-15. DOI:1038/s41598-021-85735-z
  3. Kechaykin А, Batkin, A., Sitpayeva, G., Vesselova, P., Osmonali, B., Shmakov, A. (2020). New data on genus Potentilla L. Rosaceae Juss.) in the flora of Kazakhstan. Turczaninowia (SJR=0.354, percentile-32), 23(1), 32-40.
    DOI: 14258/turczaninowia.23.1.4
  4. Osmonali1, B., Akhtaeva, N., Veselova, P., Kudabaeva, G., Kurbatova, N. (2020). Features of the anatomical structure of various species of the genus Salsola Problems of Botany of Southern Siberia and Mongolia, 19(2), 146-154. DOI: 10.14258/pbssm.2020093 (in Russian)
  5. Osmonali1, B. (2021) Species of the genus Salsola of the flora of the desert part in the valley of Syrdarya river (summary and key to identification). Problems of Botany of Southern Siberia and Mongolia, 20(2), 125-137. DOI: 10.14258/pbssm.2021130 (in Russian)
  6. Osmonali1, B., Vesselova P., Kudabayeva G., Akhtayeva N. (2022) Phytocenotic features of species of the genus Salsola (Chenopodiaceae Vent./Amaranthaceae Juss.) in the desert part of the Syrdarya river valley. Вестник Карагандинского университета, 1(105), 78-85. DOI 10.31489/2022BMG1/78-85
  7. Mukhitdinov N., Ametov A., Almerekova Sh., Abidkulova K. Ecological and biological features of plant cenopopulations of a rare and endemic Oxytropis almaatensis. KazNU bulletin. Ecology series. – Almaty: Kazakh University, 2017. — №2 (51). – P. 69-80. (in Kazakh)
  8. Eshibaev, A., Aimenova, Z., Akynova, L., Nurseitova, L., & Kopabaeva, A. (2021). The population status of Lagochilus setulosus Vved. and its biochemical composition. Ecological Questions (SJR=0.227, percentile-46) 32(2), 111-118. DOI: 12775/EQ.2021.018
    (https://www.webofscience.com/wos/woscc/full-record/WOS:000729025600009)
  9. Aimenova J.E., Yeshibaev A.A. Pharmaceutical potential of Lagochilus setulosus and technology of obtaining a biological preparation with hemostatic action using its extraction. Monography. – Shymkent university, 2019 – 130 p. ISBN 978-9965-782-30-5. (in Russian)
  10. Patent of invention № 32895 Aimenova J.E., Yeshibaev A.A. Application of Lagochilus extract as a hemostatic agent. RSE «National Institute of Intellectual Property». – 2018. Registration number 2016/1057.1 (in Russian)
  11. Turuspekov E.K., Abugalieva S.I., Almerekova Sh.S. Author’s certificate of entering information into the state register of rights to objects protected by copyright — to the database «Biodiversity of the flora of Kazakhstan» (№20035 from 01.09.2021), http://www.kazflora.kz/.

 

Results for 2022:

  1. Work on collecting populations of Salsola species, determining the species and their distribution areas in Kazakhstan, and describing phytocenoses with the participation of Salsola species. During expedition trips in the desert part of the Syrdarya River valley, more than 5 plant communities were studied with the participation or dominance of S. arbuscula and S. orientalis species of the genus Salsola. At this stage of the work, 2 populations of the species S. arbuscula (shrub) and S. orientalis (semi-shrub) were collected.
  2. Phylogenetic analysis and study of phylogenetic relationships of species of the genus Salsola using markers of nuclear and chloroplast genomes and DNA barcoding technology have been started. DNA was isolated from 59 samples of 4 populations of Salsola species: S. arbuscula — 29 samples, S. orientalis — 30 samples. The analysis of phylogenetic relationships using the technology of DNA-barcoding of species of the genus Salsola has been started. Nucleotide sequences of 4 (S. arbuscula and S. orientalis) Kazakhstan accessions of Salsola were aligned with accessions from the NCBI database.
  3. Work on drying, grinding plant material of species of the genus Salsola, and obtaining extracts for further research. For the preparation of Salsola plants, the upper flowering non-lignified branches 15-30 cm long were cut and dried in air-shade conditions.