Brief description of the project
(2021-2023)
Project title: IRN AP0925899 «Mapping of genetic factors determining resistance to Septoria nodorum based on genome-wide association study in the collection of hexaploid wheat».
Relevance.
In Kazakhstan, Septoria nodorum (SNB) is a serious problem for wheat, yield losses during epiphytoties can reach 40-50%. Breeding for host plant resistance remains the most economical, efficient and environmentally friendly strategy to control the disease. Effective combinations of resistance genes are best achieved through marker selection. The development of SNB-resistance genotypes depends on understanding host resistance mechanisms and identifying disease resistance genes. Genome wide association study (GWAS) is one of the advanced molecular and genomic selection approaches providing high resolution mapping. Identification of novel QTLs and genomic regions associated with SNB resistance, this will deepen understanding of the nature of resistance to the disease. The Project will apply the GWAS approach using DArTseq™ next generation sequencing technology, which is new and modern in the field of molecular plant breeding. Compared to SSR markers, DArT markers have advantages in terms of cost and time of genotyping. However, the highly efficient DArT technology is currently not widely used in Kazakhstan. Although Septoria is a potential hazard and harmfulness, no GWA studies have been conducted to identify genomic regions associated with resistance to Septoria in Kazakh germplasm.
The goal of the project: «Mapping of genetic factors that determine resistance to Septoria nodorum based on a genome-wide association study (GWAS) in the collection of hexaploid wheat from Kazakhstan»
Expected results:
Based on the use of the modern GWAS approach, a search for new genetic factors (QTL and DNA markers) that determine resistance to Septoria will be carried out; the genetic structure of the population has been studied, carriers of valuable alleles for their marker introgression into new wheat varieties will be identified. Promising lines and varieties of wheat resistant to isolates and selective toxins of the fungus, characterized by seedling and field resistance to Septoria in 2-contrast regions of Kazakhstan will be identified.
Scientific Supervisor of the project: A.M. Kokhmetova, Doctor of Biological Sciences (Genetics), professor, corresponding member of the National Academy of Sciences RK, head of Genetics and Breeeding Laboratory, Institute of Plant Biology and Biotechnology (IPBB), National Coordinator European Biotechnology Association (EBTNA) for Kazakhstan. Work experience is related to the study of disease resistance using Marker Assisted Selection (MAS) technology and associative mapping to improve the efficiency of the breeding for disease resistance. Since 2015 she was manager of 6 projects funded by Ministry of Education and Science and Ministry of Agriculture. Under her supervision 1 PhD, 4 PhD-doctoral, 12 master’s theses defended;1 PhD-dissertation is carried out. Hirsch Index is 9 (https://apps.webofknowledge.com/CitationReport.do?product=UA&search_mode=CitationReport&SID=E4M3qrpPTX4Xbgyu2tf&page=1&cr_pqid=6&viewType=summary). She has 300 publications, including 20 works in WOS/Scopus databases. WOS-ID: M-6618-2015; ORCHID-ID:0002-0186-7832.
Research group:
Kumarbayeva M.T., MSci (6М081100 – Plant protection and quarantine), completed PhD (6D080100 -Агрономия), scientific researcher; h-index: 4; ResearcherID: AAQ-3647-2020; OrcID: 0000-0002-5588-6772 Scopus ID: 5721361957;
Keyshilov Zhenys Sovetkhanuly, MSci (6М081100 – Plant protection and quarantine); h-index: 4; ResearcherID: AAQ-3647-2020; OrcID: 0000-0002-5588-6772 Scopus; h-index: 2; ResearcherID: DWX-6746-2022; OrcID: 0000-0003-2126-6951; Scopus ID: 57221382183;
Zhanuzak Danna Komekkyzy, MSci (6М070100 – Biotechnology), ; h-index: 4; ResearcherID: AAQ-3647-2020; OrcID: 0000-0002-5588-6772 Scopus; junior scientific researcher, h-index: 1; ResearcherID: ABE-9520-2021; Scopus ID: 57226127734; OrcID: 0000-0002-2252-0870;
Bolatbekova A.A., MSci (6M060800 – Ecology); junior scientific researcher; h-index: 1; ResearcherID: CHB-6750-2022; Scopus ID: 57226105668;
Malysheva Angelina Alexandrovna, MSci (7M05109 — biotechnology); junior scientific researcher; h-index: 2; ResearcherID: ABE-9732-2021; OrcID: 0000-0002-9840-9570; Scopus ID: 57226102193;
Kokhmetova Asiya Myrzabekovna, (08.00.05 — economics of trade); assistant, OrcID: 0000-0003-0119-0849;
Bakhytuly Kanat, master (7M05109 — biotechnology); assistant, OrcID: 0000-0001-6084-0480
List
1. Kokhmetova A, Rsaliyev A, Malysheva A, Atishova M, Kumarbayeva M, Keishilov Z. 2021. Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan // Plants. 10(11):2303. DOI: 10.3390/plants10112303 Scopus: percentile 56.
2. Kokhmetova A, Rsaliyev S, Atishova M, Kumarbayeva M, Malysheva A, Keishilov Z, Zhanuzak D, Bolatbekova A. 2021. Evaluation of Wheat Germplasm for Resistance to Leaf Rust (Puccinia triticina) and Identification of the Sources of Lr Resistance Genes Using Molecular Markers // Plants. 10(7):1484. DOI:10.3390/plants10071484 IF 3.935. WOS. Q1. Scopus: percentile 56.
3. Kokhmetova A., Kumarbayeva M., Atishova M. 2021. Identification of high-yielding wheat genotypes resistant to Pyrenophora tritici-repentis (tan spot) // Euphytica. 217:97. DOI:10.1007/s10681-021-02822-y IF 1.84. WOS. Q2. Scopus: percentile 71.
4.Madenova A.K., Kokhmetova A.M., Atishova M.N. et al. 2019. Identification of carriers of resistance to common bunt (Tilletia caries) of winter wheat // Research on Crops.20(4):782-790.DOI:10.31830/2348-7542.2019.115. Scopus, Q3. SJR 0.21. Scopus: percentile 18.
5.Kokhmetova A., Kremneva O.,Volkova G., Atishova M., Sapakhova Z. 2017. Evaluation of wheat cultivars growing in Kazakhstan and Russia for resistance to tan spot // Journal of Plant Pathology. 99(1):161-167. DOI:10.4454/jpp.v99i1.3812. IF 1.041. WOS. Q3. Scopus: percentile 31.
6. Galymbek K., Kokhmetova A.M., Akan K., Madenova A.K., Atishova M.N. 2017. Identification of germplasm of wheat on leaf rust (Puccinia recondita Rob. Ex Desm. F.sp. tritici)//Ecology, Environment and Conservation. 23(2):1211-1218. Scopus, Q4. SJR 0.14.
7. Кохметова А.М., Али С., Сапахова З., Атишова М.Н. 2018. Идентификация генотипов-носителей устойчивости к токсинам пиренофороза Ptr ToxA и Ptr ToxB Pyrenophora tritici-repentis в коллекции мягкой пшеницы//Вавиловский журнал генетики и селекции. 22(8):978¬-986. DOI:10.18699/VJ18.440. Scopus, Q4. SJR 0.15. 31-й процентиль.
8. A.Kokhmetova, M.Atishova, M.Kumarbayeva, Leonova I. 2019. Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot//Вавиловский журнал генетики и селекции. 23(7):879-886. DOI:10.18699/VJ19.562. Scopus, Q4. SJR 0.15. 31-й процентиль.
9.Kumarbayeva M., Kokhmetova A., Kovalenko N., Atishova M., Keishilov Zh., Aitymbetova K. Characterization of Pyrenophora tritici-repentis (tan spot of wheat) Races in Kazakhstan // Phytopathologia Mediterranea. – 2022(1). (Принята в печать) IF — 2.08, WOS. Q2, Scopus: процентиль 65.
10. Кеишилов Ж. С., Кохметова A.M., Кумарбаева M.T., Болатбекова A.A., Малышева A.A., Кохметова A.M. Жаздық бидайдың қоңыр тат (Puccinia recondita) ауруына солтүстік қазақстанда жүргізілген мониторингі 2019-2021 // Вестник науки Казахского агротехнического университета им. С. Сейфуллина. – 2022. – №. 1(112). – Б. 930. DOI: 10.51452/kazatu.2022.1(112).930 КОКСОН 10. Keishilov Zh.S., Kokhmetova A.M.,
11. Kumarbaeva M.T., Kokhmetova A.M., Keishilov Zh.S., Chudinov V., Zhanuzak D.K. 2022. Disease monitoring to determine the level of spread and development of the pathogen pyrenophora tritici-repentis in Kazakhstan // Herald of science of S. Seifullin KazATU. 1(112): 906. DOI:10.51452/kazatu.2022.1(112).906(КОКСОН).
12.Kumarbayeva M., Kokhmetova A., Kovalenko N., Atishova M., Keishilov Zh., Aitymbetova K. Characterization of Pyrenophora tritici-repentis (tan spot of wheat) Races in Kazakhstan // Phytopathologia Mediterranea. – 2022(1). (Принята в печать) IF — 2.08, WOS. Q2, Scopus: процентиль 65.
Results for 2022:
The response of wheat germplasm to resistance to races and host-specific toxins produced by the fungus S. nodorum was studied. Wheat germplasm resistant to S. nodorum races was identified. 107 genotypes (48.6%) resistant to the Sn2K race and 113 genotypes (51.4%) resistant to the Sn5K race were selected. A total of 154 ToxA toxin insensitive genotypes and 72 ToxB toxin resistant genotypes have been identified. A field assessment of wheat germplasm in 2 contrasting regions of Kazakhstan (Almaty and Kostanay regions) for the resistance of adult plants to S. nodorum was carried out. In the Kostanay region, 30 accessions of wheat with high field resistance to S. nodorum disease were isolated. In the Almaty region, on a natural background, 85 immune and 55 resistant genotypes were selected. Under an artificial background, the varieties Severyanka, KR12-9011, Almaly/GF92, Samgay proved to be immune, and resistant cultivars were Karaspan, Mereke 75, Saratovskaya 29, Stb-7, Yr2/Octyabrina, Bayandy, Mayra, Pamyati Azieva, Kokbiday.
