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AP 14870612 (superviser Abugalieva S.)

Brief description of the project

(2022-2024)

 Project title: IRN AP14870612 «Study of the genetic diversity in species of the genus Tulipa L. growing in Kazakhstan».

Relevance. Tulipa L. is a group of perennial geophyte monocots, one of the largest genera of the Liliaceae family. It has a great economic, floricultural, ecological importance, cultural value in many regions of the world. Most species are found in mountainous areas, 17 species grow in the steppes and adjacent deserts and semi-deserts. Most of the species are subjected to anthropogenic impact, but 18 species are included in the Red Book of Kazakhstan. The use of modern molecular genetic and genomic technologies with the involvement of bioinformatics, along with traditional botanical methods, help to refine the taxonomy and study the phylogenetic and evolutionary relationships of wild plant species.

The goal of the project: To study intra- and interspecific genetic diversity of wild-growing species of tulips in Kazakhstan using SSR markers, to determine the phylogenetic relationships of species by nucleotide sequences of DNA barcoding markers and chloroplast genome using new generation genomic technologies, as well as phytochemical traits of widespread tulip species.

Expected results:

  1. Populations of tulip species will be collected, species identity will be determined, herbariums will be compiled and digitized.
  2. Phylogenetic relationships of Tulipa species will be studied using the genetic diversity of chloroplast genome.
  3. Phylogenetic relationships of representatives of the genus Tulipa will be analyzed using markers of the nuclear and chloroplast genome.
  4. The level of intra- and interspecific genetic diversity of Tulipa species of Kazakhstan will be studied, the population structure will be determined.
  5. Widespread species of tulips will be studied for secondary metabolites.
  6. The database «Biodiversity of the flora of Kazakhstan» will be supplemented with data on botanical collections, molecular genetic data of deposited nucleotide sequences of chloroplast genomes and DNA barcoding data of species of the genus Tulipa.

Scientific Supervisor of the project: S. Abugalieva, principal researcher, Doctor of Biological Sciences, Professor. In 2017-2022 co-authored 40 scientific articles, including 29 articles in peer-reviewed foreign scientific journals indexed in Web of Science (https://www.webofscience.com/wos/author/record/2577530) and/or Scopus (H-index=8, https://www.scopus.com/authid/detail.uri?authorId=6507029042), 11 in journals recommended by the Committee for Control in the Sphere of Education and Science (CCSES) of the Ministry of Education and Science of the Republic of Kazakhstan.

Research group: 

  1. Turuspekov, Candidate of biological sciences, Professor, Head of the Laboratory of Molecular Genetics. He has >100 scientific publications, including 40 in peer-reviewed international journals, indexed in Web of Science databases (https://www.webofscience.com/wos/author/record/1523020) and/or having a CiteScore percentile in the Scopus database (H-index 14, https://www.scopus.com/authid/detail.uri?authorId=57197860996), 27 articles in 2017-2022); 41 in publications CCSES, co-author of 4 monographs, 4 scientific and methodological recommendations, databases.
  2. Almerekova, Senior Researcher, PhD (2018, Geobotany). Co-author of 35 scientific papers, incl. 12 articles, including 7 articles in peer-reviewed journals indexed in Web of Science (H-index=4, https://www.webofscience.com/wos/author/record/9617158) and Scopus (H-index=4, https ://www.scopus.com/authid/detail.uri?authorId=57196952712), and 7 articles in journals, CCSES MES RK.
  3. Amalova, MS (Geobotany), researcher, co-author of 5 articles (H-index 2, https://www.scopus.com/authid/detail.uri?authorId=57213623570) in Scopus/WoS, and 3 articles in CCSES.
  4. Yermagambetova, MS, PhD-student, junior researcher, has 1 article in Scopus/WoS.
  5. Podzorova, Bachelor, laboratory assistant at IPBB, master student of the 2nd year of al-Farabi Kazakh National University, ORCID ID 0000-0002-9521-5754.

 List of publications of the project’s participants (2017-2022)

  1. Abugalieva S., Volkova L., Genievskaya Y., Ivaschenko A., Kotukhov Y., Sakauova G., Turuspekov Y. (2017). Taxonomic assessment of Allium species from Kazakhstan based on ITS and matK markers. BMC Plant Biology (IF=4.96, quartile-Q1; SJR=1.378, percentile-87), 17(2), 51-60. doi: 10.1186/s12870-017-1194-0;
  2. Turuspekov Y., Genievskaya Y., Baibulatova A., Zatybekov A., Kotuhov Y., Ishmuratova M., Imanbayeva A., Abugalieva S. (2018). Phylogenetic taxonomy of Artemisia species from Kazakhstan based on matK analyses. Proceedings of the Latvian Academy of Sciences. Section B: Natural, Exact, and Applied Sciences (Latvia) (SJR=0.168, percentile-39), 72(1), 29. https://doi.org/10.1515/prolas-2017-0068
  3. Almerekova S., Mukhitdinov N., Abugalieva S. (2017). Phylogenetic study of the endemic species Oxytropis almaatensis (Fabaceae) based on nuclear ribosomal DNA ITS sequences. BMC Plant Biology (IF= 4.96, quartile-Q1; SJR=1.378, percentile-87), 17(1), 1-9. https://doi.org/10.1186/s12870-017-1128-x
  4. Almerekova S., Shchegoleva N., Abugalieva S., Turuspekov Y. (2020). The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae). PloS one (IF=3.788, quartile-Q2; SJR=0.990, percentile-92), 15(10), e0240121. https://doi.org/10.1371/journal.pone.024012;
  5. Almerekova, S., Abugalieva, S.*, Mukhitdinov, N. (2018). Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan. Vavilovskii Zhurnal Genetiki i Selektsii= Vavilov Journal of Genetics and Breeding. (SJR=0.188, percentile-38), 22(2), 285-290.
  6. Genievskaya Y., Abugalieva S., Zhubanysheva A., Turuspekov Y. (2017). Morphological description and DNA barcoding study of sand rice (Agriophyllum squarrosum, Chenopodiaceae) collected in Kazakhstan. BMC Plant Biology (IF=4.96, quartile-Q1; SJR=1.378, percentile-87), 17(1), 1-8. https://doi.org/10.1186/s12870-017-1132-1;
  7. Özek G.; Schepetkin I.A.; Yermagambetova M.; Özek T.; Kirpotina L.N.; Almerekova S.S.; Abugalieva S.I.; Khlebnikov A.I.; Quinn M.T. (2021) Innate Immunomodulatory Activity of Cedrol, a Component of Essential Oils Isolated from Juniperus Molecules (IF=4.588, quartile-Q2; SJR=0.990, percentile-74), 26, 7644.
  8. Turuspekov Y., Abugalieva S., Ermekbayev K., Sato K. (2014). Genetic characterization of wild barley populations (Hordeum vulgare spontaneum) from Kazakhstan based on genome wide SNP analysis. Breeding Science, (IF= 2.63, quartile–Q2; SJR=0.799, percentile-74) 64(4), 399-403.
  9. Sumbembayev A., Abugalieva S., Danilova A., Matveyeva E., Szlachetko D. (2021). Flower morphometry of members of the genus Dactylorhiza Necker ex Nevski (Orchidaceae) from the Altai Mountains of Kazakhstan. Biodiversitas Journal of Biological Diversity (SJR=0.257, percentile-40), 22(8). https://doi.org/10.13057/biodiv/d220855
  10. Almerekova S., Favarisova N., Turuspekov Y., Abugalieva S.* (2020). Cross-Genera Transferability of Microsatellite Markers and Phylogenetic Assessment of Three Salsola Species from Western Kazakhstan. Proceedings of the Latvian Academy of Sciences. Section B: Natural, Exact, and Applied Sciences (Latvia) (SJR=0.168, percentile-39), 74 (5), 325-334. https://doi.org/10.2478/prolas-2020-0049.
  11. Amalova A., Kurmanbaeva M., Turuspekov E., Ivashchenko A., Abidkulova K (2018). Ontogenetic structure of cenopopulations Tulipa оstrowskiana Regel in Zailiyskiy Alatau. KazNU Bulletin. Ecological series, 56(3), 101-114. (in Russian)
  12. Favarisova, N., Kusmangazinov A., Karelova D., Yermagambetova M., Abugalieva S.I. 2020. Optimization of PCR Conditions for Agriophyllum, Haloxylon and Salsola Microsatellite Markers. International Journal of Biology and Chemistry 13 (1):57-68. https://doi.org/10.26577/ijbch.2020.v13.i1.06.
  13. Orazov A.E., Mukhitdinov N.M., Myrzagalieva A.B., Turuspekov Y.K., Shramko G., Kubentaev S.A. Distribution and characteristics of cenopopulations of Amygdalus ledebouriana on the territory of the Narymsky ridge // KazNU Bulletin. Biological series (IF=0.043). 2019. №1(78). P.36-45. DOI: https://doi.org/10.26577/eb-2019-1-1401 (in Russian)
  14. Turuspekov E.K., Abugalieva S.I., Almerekova Sh.S. Author’s certificate of entering information into the state register of rights to objects protected by copyright — to the database «Biodiversity of the flora of Kazakhstan» (№20035 from 01.09.2021),http://www.kazflora.kz/.

Results for 2022:

  1. Starting isolation and purification of the DNA of available populations of Tulipa species growing in Kazakhstan. At this stage of the project, DNA has been isolated from 110 samples of 6 populations of Tulipa species native to Kazakhstan.
  2. Starting identification and deposition of nucleotide sequences of ITS markers of the nuclear genome of species of the genus Tulipa. Depositing the nucleotide sequences of ITS markers of the nuclear genome of species of the genus Tulipa in the international database NCBI (National Center for Biotechnology Information).
  3. Starting to study the level of intra- and interspecific genetic diversity of populations of endemic, rare and endangered Tulipa species of Kazakhstan using SSR markers, to determine the population structure. At this stage of the project, the PCR conditions for SSR markers of tulips were optimized in terms of the primer annealing temperature, the concentration of dNTPs (deoxynucleoside triphosphates) and Mg2+ in the PCR reaction mixture. The results will be used to study the level of genetic diversity of populations of endemic, rare and endangered species of tulips.