Laboratory of Germplasm Cryopreservation

%d0%b7%d0%b0%d0%b2-%d0%bb%d0%b0%d0%b1-%d0%ba-%d0%b1-%d0%bd-%d1%81-%d0%b2-%d0%ba%d1%83%d1%88%d0%bd%d0%b0%d1%80%d0%b5%d0%bd%d0%ba%d0%beHead of Laboratory: Kushnarenko Svetlana Veniaminovna, Candidate of Biological Sciences (PhD), Associate Professor.

Tel.: 393-55-90; 394-75-54. E-mail: svetlana_bio@mail.ru

She has been working as head of the laboratory since 2000. Graduated from the Biology Faculty of Moscow State University on a specialty “Biochemistry” (1984). Defended her Candidate’s dissertation on a specialty “Plant Physiology and Biochemistry” in 1990. In 2015 she was awarded an Associate Professor.

She is a specialist in the sphere of plant biotechnology, physiology and biochemistry. An author of over 130 research papers on plant tissue culture, micropropagation, cryopreservation, also the component composition and biological activity of essential oils extracted from plants of Kazakhstan.

Supervised one candidate of sciences, now she is the supervisor of two PhD-students.

A winner of the President Grant of the Republic of Kazakhstan (2003). She was awarded with the Honorary Diploma of the MES RK for contribution to development of science in Kazakhstan (2014).

Membership in professional societies:

European Biotechnology Thematic Network Association

Society for In vitro Biology

International Association of Sexual Plant Reproduction Research

Society of Plant Physiologists of Kazakhstan

Society of Plant Physiologists of Russia

Laboratory Staff:

  • Rakhimbayev Izbassar Rakhimbayevich, Chief research scientist, Member of the NAS of the RK, Professor, Doctor of Biological Sciences
  • Kovalchuk Irina Yurievna, Leading research scientist, Candidate of Agricultural Sciences
  • Romadanova Natalya Vladimirovna, Leading research scientist, Candidate of Biological Sciences
  • Turdiyev Timur Tuygunovich, Leading research scientist, Candidate of Biological Sciences
  • Utegenova Gulzhakhan Abduzhalilovna, Junior research scientist, Master of Biology, PhD-student
  • Karasholakova Lyazzat Naushabayevna, Junior research scientist, Master of Biology, PhD- student
  • Frolov Sergey Nikolayevich, Junior research scientist
  • Mishustina Svetlana Aleksandrovna, Junior research scientist, Master of Biology
  • Madiyeva Gulnara Akimbekovna, Junior research scientist, Bachelor of Biology
  • Aralbayeva Moldir Malikovna, Senior laboratory assistant, Bachelor of Biology
  • Zholamanova Saule Zhanibekkyzy, Senior laboratory assistant, Bachelor of Biology

Main research areas of the laboratory

Laboratory activities are aimed at addressing a number of actual tasks of fundamental and applied relevance:

  • Study of physiological and genetic regularities of low-temperature exploration on plant cells: cytomorphology, ultrastructure of cell organelles, cytochemistry, morphogenesis, regeneration.
  • Improvement of cryopreservation methods in compliance with the type of explant and species specificity of plant material.
  • Development of biotechnology regulations of the cryopreservation in compliance with biological peculiarities of cultures.
  • Creation of a bank of in vitro plants at +4°C for medium-term storage of germplasm of economically important, rare and endangered plants.
  • Creation of a cryobank in liquid nitrogen at -196°С for long-term storage of germplasm of economically important, rare and endangered plants.
  • Development of biotechnology for biologically active agents.

Main results of activities

  • In vitro plant collection of 178 cultivars, hybrids and wild forms was created, including 36 – apple, 38 – pear, 30 – raspberry, 12 – black currant, 20 – barberry, 10 – honeysuckle, 32 – potato.
  • Seed collections of wild forms of apricot, apple, honeysuckle and barberry, as well as cultivars of rice were formed at +4°C and -20°C.
  • Biotechnological regulations for cryopreservation of apical meristems of apple, currant, raspberry, apricot, grape, potato, honeysuckle, barberry and strawberry in liquid nitrogen were developed on the basis of improved cryopreservation methods.
  • The first national plant cryobank was created where cryogenic collection of 620 cultivars, hybrids and wild forms of apple, currant, raspberry, strawberry, honeysuckle, barberry, potato and rice is contained in liquid nitrogen at ultra-low temperature (-196ºC).
  • An electronic database of genotypes on rice and grape genotypes of domestic and foreign selection was created with connection to international database Botanic Gardens Conservation International (BGCI) http://www.bgci.org/garden.php?id=4756
  • Innovation patents for the invention were obtained:

— Innovation patent for the invention № 28526 from 16.05.2014, Kazakhstan. A method for decontaminating seeds of cereal cultivars / Turdiyev T.T., Mamonov L.K., Kovalchuk I.Y. et al.

— Innovation patent for the invention № 29084 from 20.10.2014, Kazakhstan. A method for viability recovery and germination increase of rice seeds after the long storage / Turdiyev T.T., Mamonov L.K., Kovalchuk I.Y. et al.

— An application for utility model № 14794 from 31.07.2015. The Republic of Kazakhstan. A method for producing of rehabilitated apple seedlings using cryotherapy / Romadanova N.V., Kushnarenko S.V.

Projects and Programs

  • 2015-2017. Project 2117/GF4 “Pharmacological effects of essential oils and their components from plants of Kazakhstan”. Project Manager – Kushnarenko S.V.
  • 2015-2017. Project 1783/GF4 “Development of technology for the cryogenic germplasm preservation of valuable barberry species and forms – a source of biologically active substances”. Project Manager – Romadanova N.V.
  • 2015-2017. Project 2174/GF4 “Development of biotechnological regulations for germplasm conservation of varietal and species diversity of pear for use in the breeding process on the creation of new highly productive and resistant cultivars”. Project Manager – Turdiyev T.T.
  • 2013-2015. Project 0504/GF3 “Identification and preservation of new sources of essential oils among the poorly known species of Kazakhstan Altai flora”. Project Manager – Kushnarenko S.V.
  • 2013-2015. Project 0491/GF3 “Creation of a cryogenic bank of promising cultivars and clonal rootstocks of apple on the basis of biotechnology”. Project Manager – Romadanova N.V.
  • 2012-2014. Project 0047/GF “Creation of a cryogenic bank of potato germplasm for reliable conservation and efficient utilization of genetic resources in seed and breeding practice of Kazakhstan”. Project Manager – PhD, Kushnarenko S.V.
  • 2012-2014. Project 0046/GF “Development of biotechnology for cryopreservation of cell cultures and creation of a germplasm bank of domestic and foreign grape cultivars”. Project Manager – Kovalchuk I.Y.
  • 2012-2014. Project 0040/GF “Development of biotechnological methods for long-term preservation and study of the rice collection in Kazakhstan”. Project Manager – Mamonov L.K., Turdiyev T.T.
  • 2012-2014. Project “Development of biotechnologies for production of biologically active preparations from Kazakhstan honeysuckle species” within the scope of the scientific and technical program “Biotechnologies for drug development, dietary supplements and specialty foods and other materials on the basis of cultural, introduced and wild species of Kazakhstan flora”. Project Manager – Kushnarenko S.V.
  • 2011-2013. K-1920 ISTC Project “Estimation of Apricot and Pear Genetic Diversity and Germplasm Preservation in Kazakhstan”. Project Manager – Kovalchuk I.Y.

Scientific cooperation with international and domestic organizations

  • United States Department of Agriculture, Agricultural Research Services (USDA, ARS), National Clonal Germplasm Repository, Corvallis, Oregon, USA (Dr. B.Reed).
  • Anadolu University, Faculty of pharmacy, Department of pharmacognosy, Eskisehir, Turkey (Prof., Dr. T. Ozek, Assoc. Prof. Dr. G. Ozek).
  • Montana State University, Department of microbiology and immunology, Bozeman, USA (Dr. I. Schepetkin).
  • State Scientific Institution All-Russian Research Institute named after Vavilov N.I. of the Russian Academy of Agricultural Sciences, Saint Petersburg.
  • Botanical Garden of Peter the Great, The Federal state budgetary scientific institution, Botanical Institute named after Komarova V.L. of Russian Academy of Sciences (BIS RAS), Saint Petersburg.
  • State budgetary Institution of the Republic of Crimea “Order of Red Banner of Labour Nikitsky Botanical Garden – National scientific center”.
  • Federal Agency of scientific organizations of Russia, Federal State budgetary scientific institution “All-Russian Scientific Research Institute of Rice», Krasnodar.
  • JSC “Lesnoi pitomnik” of SC MES RK.
  • LLP “Kazakh Research Institute of Horticulture and Viticulture” of Ministry of Agriculture of the Republic of Kazakhstan, Almaty.
  • LLP “Kazakh Research Institute of Rice named after Ibrai Zhakhayev” of Ministry of Agriculture of the Republic of Kazakhstan, Kyzylorda.

Scientific — pedagogical activity

Research works were made in the laboratory and bachelor’s, master’s and PhD degrees has been obtained:

Bachelor degree course:

Matakova G.N. – Al-Farabi KazNU, specialty 5В070100 – «Biotechnology», 2013.

Bekebayeva M.О. – Al-Farabi KazNU, specialty 5В070100 – « Biotechnology », 2013.

Karabalin А.А. – Al-Farabi KazNU, specialty 5В070100 – « Biotechnology », 2013.

Zholamanova S.Zh. – Al-Farabi KazNU, specialty 5В070100 – «Biotechnology», 2016.

Madiyeva G.А. – KazSWPU, specialty 5B011300 – «Biology», 2016.

Master degree course:

Karasholakova L.N. – Al-Farabi KazNU, specialty 6М061300 – «Geobotany», 2013.

Bekebayeva M.О. – Al-Farabi KazNU, specialty 6М070100 – «Biotechnology», 2015.

PhD degree course:

Zhumagulova Zh.B., KazNAU, specialty 6D080900 – «Horticulture», 2012-2015.

Karasholakova L.N. – Al-Farabi KazNU, specialty 6D061300 – « Geobotany », 2013-2016.

Kabylbekova B.Zh., KazNAU, specialty 6D080900 – «Horticulture», 2014-2017.

Utegenova G.A. – Al-Farabi KazNU, specialty 6D070100 – «Biotechnology», 2014-2017.

Foreign trainings, honorary academic degrees, awards, scholarships

  • 2016, 2015. Karasholakova L.N. Training at Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, Eskisehir, Turkey.
  • Turdiyev T.T., Madiyeva G.A. Training in statistical analysis and experimental design, National Clonal Germplasm Repository, Corvallis, USA.
  • Utegenova G.A. Training at Montana State University, Bozeman, USA.
  • Romadanova N.V. Training at the State Scientific Institution All-Russian Research Institute named after Vavilov N.I. of the Russian Academy of Agricultural Sciences, Saint Petersburg, on the theme “Plant genetic resources and their use in crop breeding”.
  • Karasholakova L.N. Training under the program “Central Asia Biodiversity Network» in Germany, Greifswald, at the University named after Ernst-Moritz-Arndt.
  • Matakova G.N. – scholarship holder of the special one-time scholarship of Kazakhstan Alumni Association of the William George Fulbright Program.
  • Bekebayeva M.O. – the diploma of the first degree at the international conference of students and young scientists “World of Science» on the 20th anniversary of the Republic of Kazakhstan.
  • Rakhimbayev I.R. – was awarded the medal “Bolshaya Zolotaya Medal” of NAS RK.
  • Rakhimbayev I.R. – was awarded the Order “Kurmet”.
  • Rakhimbayev I.R. – was awarded the medal “For achievements in science”.
  • Kushnarenko S.V. – the President Grant of the Republic of Kazakhstan for supporting young scientists to be trained in foreign research centers.

Main publications

  • Кushnarenko S.V., КаrasholakovаN., Оzек G., Аbidkulova К.Т., Мukhitdinov N.М., Bаsеr К.H.C., Оzек Т. Investigation of essential oils from three natural populations of Lonicera iliensis // Chemistry of Natural Compounds. – 2016. – V. 52 (4). – P. 751-753.
  • Romadanova N.V., Mishustina S.A., Gritsenko D.A., Omasheva M.Y., Galiakparov N.N., Reed B.M., Kushnarenko S.V. Cryotherapy as a method for reducing the virus infection of apples (Malus) // CryoLetters. – 2016. – V. 37, No. 1. – P. 1-9.
  • Romadanova N.V., Mishustina S.A., Matakova G.N., Kuhsnarenko S.V., Rakhimbaev I.R., Reed B.M. In vitro collection of Malus shoot cultures for cryogenic bank development in Kazakhstan // Acta Horticulturae. – 2016. – V. 1113. – Р. 271-277.
  • Schepetkin I.A., Kushnarenko S.V., Özek G., Kirpotina L.N., Utegenova G.A., Kotukhov Y.A., Danilova A.N., Özek T., Başer K.H.C., Quinn M.T. Inhibition of Human Neutrophil Responses by Essential Oil of Artemisia kotuchovii and its Constituents – J. Agric. Food Chem. – 2015. – V. 63. – P. 4999-5007.
  • Турдиев Т.Т., Ковальчук И.Ю., Успанова Г.К., Чуканова Н.И., Фролов С.Н. Оптимизация клонального микроразмножения для сохранения генофонда растений груши // Вестник КазНУ, Серия биологическая. – 2015. – № 3. – С. 356-362.
  • Утегенова Г.А., Кушнаренко С.В. Биологическая активность эфирных масел и их компонентов // Доклады Национальной академии наук Республики Казахстан. – 2015. – № 4. – С. 146-153.
  • Кушнаренко С.В., Утегенова Г.А., Озек Г., Озек Т., Котухов Ю.А., Данилова А.Н., Сатеков Е.Я. Каталог эфирных масел растений Восточного Казахстана. – Алматы. – 2015. – 72 с.
  • Kushnarenko S.V., Romadanova N.V., Bekebaeva M.O., Aleksandrova A.M, Reed B.M., Combined ribavarin treatment and cryotherapy for efficient potato vitus M and potato virus S eradication in potato in vitro plantlets // Cryobiology. – 2015. – V. 71 (3) – P 569-570.
  • Кушнаренко С.В., Ромаданова Н.В., Бекебаева М.О., Матакова Г.Н. Усовершенствование регламента криоконсервации апикальных меристем для создания криобанка сортов и гибридов картофеля // Биотехнология. Теория и практика. – 2015. – №2. – С. 35-41.
  • Ромаданова Н.В., Мишустина С.А., Карашолакова Л.Н., Аралбаева М.М., Кабулова Ф.Д., Абидкулова К.Т., Кушнаренко С.В. Введение в культуру in vitro дикорастущих видов Berberis флоры Казахстана и Узбекистана // Вестник КазНУ. Серия биологическая. – 2015. – №3. – С. 346-354.
  • Kovalchuk I., Zhumagulova Zh., Turdiev T., Reed B.M. Growth medium alterations improve in vitro cold storage of pear germplasm // CryoLetters. – 2014. – V. 35. – N. 3. – P. 197-203.
  • Kovalchuk I., Turdiev T., Mukhitdinova Z., Frolov S., Reed B.M., Kairova G. New techniques for rapid cryopreservation of dormant vegetative buds // Proceedings of the Second International Symposium on Plant Cryopreservation. Ford Collins. Colorado. USA. Acta Horticulturae. – 2014. – V. 1039. – P. 137-146.
  • Kovalchuk I., Turdiev T., Mukhitdinova Z., Frolov S., Reed B.M. Cryopreservation of native Kazakhstan apricot (Prunus armeniaca L) seeds and embryonic axes // CryoLetters. – 2014. V. – N. 2. – P. 83-89.
  • Кушнаренко С.В., Ромаданова Н.В., Аралбаева М.М., Матакова Г.Н., Бекебаева М.О., Бабисекова Д.И. Создание коллекции in vitro сортов и гибридов картофеля как исходного материала для криоконсервации // Биотехнология. Теория и практика. – 2013. – №1. – С. 28-33.
  • Ромаданова Н.В., Мишустина С.А., Матакова Г.Н., Рахимбаев И.Р., Кушнаренко С.В. Введение в культуру in vitro и микроклональное размножение перспективных сортов, клоновых подвоев и дикорастущих форм яблони // «Ізденістер, нәтижелер, Исследования, результаты». – 2013. – № 3 (059). – С. 142-149.
  • Kushnarenko S.V., Reed B.M., Normah M.N. Cryopreservation of in vitro grown shoot tips // In: Plant Tissue Culture, Development, and Biotechnology. Eds. R.N. Trigiano and D.J. Gray. CRC Press. Taylor & Francis Group. – USA. – 2011. – P. 497-505.
  • Kushnarenko S., Salnikov E., Nurtazin M., Mukhitdinova Z., Rakhimbaev I., Reed B. M. Characterization and Cryopreservation of Malus sieversii Seeds // The Asian and Australasian Journal of Plant Science and Biotechnology – 2010. – V. 4 (Special Issue 1). – P. 5-9.
  • Kushnarenko S., Kovalchuk I., Mukhitdinova Z., Rakhimova E., Reed B.M. Ultrastructure study of apple meristem cells during cryopreservation // The Asian and Australasian Journal of Plant Science and Biotechnology – 2010. – V. 4 (Special Issue 1). – P. 10-20.
  • Kovalchuk I.Y., Turdiev T.T., Kushnarenko S.V., Rakhimbaev I.R., Reed B.M. Cryopreservation of raspberry cultivars: testing techniques for long-term storage of Kazakhstan’s plant germplasm // Asian and Australasian Journal of Plant Science and Biotechnology. – V. 4 (Special Issue 1). – 2010 – P. 1-4.

Benefits

  • Obtaining rehabilitated super-elite seedlings of fruit, berry and ornamental plants for nurseries and farms.
  • Quick propagation of hard propagated trees for urban landscaping, backyard farms and setting up of breed shed and plantations using biotechnological methods.
  • Obtaining virus-free potato of domestic and foreign cultivars for seed farms.
  • Organization of specialized biotechnological laboratories for plant micropropagation and cryopreservation.
  • Carrying out of scientific trainings on the topics:

— Plant micropropagation.

— Cryopreservation of plant germplasm.

Brand products

  • Improved super-elite plant material of apple, pear, grape, raspberry, currant, strawberry, honeysuckle and barberry.
  • Virus-free seed potat

 

Академик Рахимбаев И.Р.

Академик Рахимбаев И.Р.

Закладка образцов растений в криобанк

Закладка образцов растений в криобанк

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Гидродистилляция эфирных масел

Гидродистилляция эфирных масел

Работа в ламинар-боксе

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