Laboratory of Molecular Biology

Head of the Laboratory: 

Gritsenko Dilyara Alexandrovna, PhD

E-mail: d.kopytina@mail.ru

Scopus

Researcher ID Web of Science

ORCID ID 

Researchgate

 PROFESSIONAL BACKGROUND :

Head of the laboratory since 2019. Bachelor’s degree in Engineering and Technology from KazNU named after al-Farabi (2006-2010). Master’s degree in Natural Sciences from KazNU named after al-Farabi (2010-2012). PhD in 2019 with a thesis on «Creation of a viral vector for the production of recombinant proteins in plants and the study of their expression efficiency». Internship at the Tolkovsky Laboratory at the Volcani Center Plant Protection Institute in Israel. Awarded the «Nazarbayev Scholarship» by the President for academic excellence and high achievements in research in 2011. Author of over 50 scientific works, Hirsch index – 5.

 

MISSION OF THE LABORATORY:

Ensuring the country’s food security through fundamental and applied research in plant breeding, rational use of genetic resources, and plant protection.

Developing modern technologies and knowledge for protecting strategically important agricultural crops and applied research in diagnostics systems, detection, and identification of economically important phytopathogens, quarantine pests and pathogens, and genetic passportization of agricultural crops.  

LABORATORY TEAM:

Dilyara Gritsenko, PhD (Head of the Laboratory)

G.K. Nizamdinova, PhD (Senior Researcher)

M.A. Khusnitdinova, PhD (Senior Researcher)

A.S. Pozharskiy (Researcher)

A.I. Kapytina (Junior Researcher)

N.M. Kerimbek (Junior Researcher)

A.K. Taskuzhina (Junior Researcher)

V.S. Kostyukova (Junior Researcher)

R.T. Kenzhebekova (Junior Researcher)

M.V. Kolchenko (Laboratory Assistant)

K.S. Adilbayeva (Laboratory Assistant)

R.M. Moiseev (Laboratory Assistant)

A.Mendybayeva (Laboratory Assistant)

A.N. Makhambetov (Laboratory Assistant)

Z.K. Dairbekova (Laboratory Assistant)

M.I. Yelchaninov (Laboratory Assistant)

  

MAIN RESEARCH FOCUS OF THE LABORATORY:

Identification and molecular-genetic research of phytopathogens and pests.

Development of recombinant molecules for biotechnology.

Whole-genome studies of pathogens and pests of plants.

Study of phytopathological and genetic aspects of populations of wild and cultivated apple.

Genetic potential of potato resistance to major diseases and pests.

Study of the genetic diversity of the local breeding collection of tomato varieties.

Study of the genetic potential of sugar beet and its phytopathological status.

Collaboration and services in molecular genetic research.

  

MAIN RESULTS OF THE ACTIVITIES:

Established a system for regenerating various grape varieties from meristematic and apical tissue.

Developed a system for simultaneous diagnostics of six grape viruses (ArMV GFkV GFLV GVA GLRV-1 GLRV-3).

Developed a system for simultaneous diagnostics of five apple viruses (ASGV ASPV ACLSV ApMV ToRSV).

Conducted an analysis of the genetic diversity of wild apple populations in Kazakhstan using DNA markers.

Genotyped technical and table grape varieties of domestic and foreign selection cultivated in Kazakhstan.

Developed viral vectors based on the genome of grapevine virus A for the expression of heterologous proteins in plants.

Developed a highly sensitive diagnostics system for Erwinia amylovora (bacterial fire blight of fruit and berry crops) based on the loop-mediated isothermal amplification of nucleic acids method.

Established a GMO detection system.

 

SERVICES:

Detection of viral pathogens:

Grapevine virus A

Grapevine short node virus

Grapevine leafroll-associated virus-1 and 3

Arabis mosaic virus

Grapevine fleck virus

Apple chlorotic leaf spot virus

Apple stem pitting virus

Apple stem grooving virus

Apple mosaic virus

Tomato ringspot virus

Tomato brown rugose fruit virus (ToBRFV)

Barley stripe mosaic virus

Wheat streak mosaic virus

Wheat mosaic emaravirus

Beet necrotic yellow vein virus

Raspberry leaf mottle virus (RLMV)

Raspberry leaf blotch virus (RLBV).

 

Detection of bacterial pathogens:

Bacterial fire blight of fruit crops, Brown rot of potato.

 

Detection of fungal pathogens:

Moniliosis of fruit crops.

 

Identification of insects using PCR:

Lymantria Dispar Dispar

Nematodes.

 

 

KEY PUBLICATIONS (2019-2024):

           Kapytina A, Kolchenko M, Kerimbek N, Pozharskiy AS, Nizamdinova G, Taskuzhina A, Adilbayeva K, Khusnitdinova M, Amidullayeva M, Moisseyev R, et al. Distribution of Wheat-Infecting Viruses and Genetic Variability of Wheat Streak Mosaic Virus and Barley Stripe Mosaic Virus in Kazakhstan. Viruses. 2024; 16(1):96. https://doi.org/10.3390/v16010096

1. Gritsenko D. et al. First evaluation of genetic diversity among honeybee populations in Kazakhstan // Apidologie. 2023. 54, № 6. P. 61.
2. Khusnitdinova M. et al. Problems and prospects of sugar beet cultivation in Kazakhstan (Changed title according to reviewers’ comments) // Agronomy Research. 2023. Vol. 21.
3. Kairova G. et al. Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields // Applied Sciences. 2023. Vol. 13, № 20. P. 11530.
4. Kolchenko M. et al. Wild Malus niedzwetzkyana Dieck ex Koehne as a Genetic Resource for Fire Blight Resistance // Horticulturae. 2023. Vol. 9, № 10. P. 1066.
5. Pozharskiy A. et al. Genetic structure and genome-wide association study of the traditional Kazakh horses // animal. 2023. P. 100926.
6. Kolchenko M. et al. Genetic Characterization of Raspberry Bushy Dwarf Virus Isolated from Red Raspberry in Kazakhstan: 4 // Viruses. Multidisciplinary Digital Publishing Institute, 2023. Vol. 15, № 4. P. 975.
7. Gritsenko D. et al. Investigation of mutation load and rate in androgenic mutant lines of rapeseed in early generations evaluated by high-density SNP genotyping // Heliyon. Elsevier, 2023. Vol. 9, № 3.
8. Pozharskiy A. et al. Genetic diversity of the breeding collection of tomato varieties in Kazakhstan assessed using SSR, SCAR and CAPS markers // PeerJ. 2023. Vol. 11. P. e15683.
9. Kairova G, Daulet N, Solomadin M, Sandybayev N, Orkara S, Beloussov V, Kerimbek N, Gritsenko D, Sapakhova Z. Identification of Apple Varieties Resistant to Fire Blight (Erwinia amylovora) Using Molecular Markers. Horticulturae. 2023; 9(9):1000. https://doi.org/10.3390/horticulturae9091000
10. Pozharskiy A. et al. MLO Proteins from Tomato (Solanum lycopersicum L.) and Related Species in the Broad Phylogenetic Context: 12 // Plants. Multidisciplinary Digital Publishing Institute, 2022. Vol. 11, № 12. P. 1588.
11. Gritsenko D. et al. Apple varieties from Kazakhstan and their relation to foreign cultivars assessed with RosBREED 10K SNP array // Europ.J.Hortic.Sci. 2022. Vol. 87, № 1.
12. Gritsenko D. et al. Development of primer sets for detection of Raspberry leaf blotch virus and Raspberry leaf mottle virus by multiplex RT-PCR // Eurasian Journal of Applied Biotechnology. 2022. № 1.
13. Pozharskiy A. et al. Screening a collection of local and foreign varieties of Solanum lycopersicum L. in Kazakhstan for genetic markers of resistance against three tomato viruses // Heliyon. 2022. Vol. 8, № 8. P. e10095.
14. Zhigailov, A. V., Stanbekova, G. E., Nizkorodova, A. S., Galiakparov, N. N., Gritsenko, D. A., Polimbetova, N. S., … & Iskakov, B. K. (2022). Phosphorylation of the alpha-subunit of plant eukaryotic initiation factor 2 prevents its association with polysomes but does not considerably suppress protein synthesis., 111190.
15. S. Pozharskiy, K.P. Aubakirova, Gritsenko D.A., N.I. Tlevlesov, N.Zh. Karimov, N.N. Galiakparov, N.A. Ryabushkina. (2020) Genotyping and morphometric analysis of Kazakhstani grapevine cultivars versus Asian and European cultivars// Genet. Mol. Res., vol. 19, no. 1. DOI: 10.4238/gmr18482
16. Pozharskiy, A., Khamzina, A., Gritsenko, D., Khamzina, Z., Kassymbekova, S., Karimov, N., … & Tlevlesov, N. (2020). SNP genotyping and population analysis of five indigenous Kazakh sheep breeds. Livestock Science, 241, 104252.
17. Karpova, A. Alexandrova, E. Yeriskina, R. Kryldakov, D. Gritsenko, N. Galiakparov, B. Iskakov Andean and Ordinary Strains of Potato Virus S Infecting Potatoes in Southern Kazakhstan // Plant Disease, Vol.104, No.2, P. 599, 2020. https://doi.org/10.26577/ijbch.2020.v13.i1.13.
18. Gritsenko, D.. et al. Simultaneous detection of five apple viruses by RT-PCR. International Journal of Biology and Chemistry, [S.l.], v. 13, n. 1, p. 129-134, aug. 2020. ISSN 2409-370X.
19. Gritsenko, D.A., Kenzhebekova, R.T., Deryabina, N.D., Galiakparov N.N. Development of a “deconstructed” vector based on the genome of grapevine virus A // Plant Biotechnol Rep. -2019. — Vol. 13. — P. 169-177. https://link.springer.com/article/10.1007/s11816-019-00528-1
20. Gritsenko, D., Pozharsky, A, Deryabina, N., Kassenova, A, Galiakparov N. Genetic analysis of hemagglutinin proteins of H3 and H1 subtypes in Kazakhstan // Genetika, 2019. DOI: 10.2298/GENSR1902511G.

 

Patents

1. D.A. Gritsenko, I.S. Gritsenko. Copyright certificate for the computer program DNARNAref, IS 009529, No. 1890 (2017), MYURK.
2. A.O. Sagitov, B.A. Duisembekov, A.B. Zhanarbekova, B.K. Kopzhasarov, G. Kaldybekkyzy, A.A. Jaimurzina, A.S. Dinasilov, Z.B. Beknazarova, A.M. Sarbasova, A.A. Darubaev, A.A. Seisenova, D.M. Dzhumakhan, T.S. Sambetova, D.A. Gritsenko. Copyright certificate «Applied scientific research in the field of protection and quarantine of plants to ensure phytosanitary security of the Republic of Kazakhstan (scientific work)», No. 0645 (2018).
3. N.N. Galiakparov, M. Omashova, D.A. Gritsenko. Patent for the invention «Set of synthetic oligonucleotides for the detection of apple viruses by RT-PCR», No. 33634 (2019). (https://gosreestr.kazpatent.kz/Invention/DownLoadFilePdf?patentId=283865&lang=ru)
4. N.N. Galiakparov, D.A. Gritsenko, N.N. Deryabina. Patent for the invention «Viral vector for transient expression of heterologous proteins in plants», 33632 (2019). (https://gosreestr.kazpatent.kz/Invention/DownLoadFilePdf?patentId=283863&lang=ru)
5. N.N. Galiakparov, D.A. Gritsenko, N.N. Deryabina. Utility model patent No. 4583 (2019) «T-vector for cloning PCR products of different sizes». (https://gosreestr.kazpatent.kz/Utilitymodel/Details?docNumber=316755)
6. D.A. Gritsenko, N.N. Galiakparov. Utility model patent «Complex of antigens of influenza virus subtype H3N8», No. 5764 (2020).
7. D.A. Gritsenko, A.I. Kapytina, N. Kerimbek. Utility model patent No. 7018 (2022) «Set of synthetic oligonucleotides for the detection of raspberry ring spot virus».
8. D.A. Gritsenko, A.S. Pozharskiy, V.S. Kostyukova, K. Adilbayeva. Utility model patent No. 7296 (2022) «Set of synthetic oligonucleotides for the detection of tomato brown rugose fruit virus».
9. D.A. Gritsenko, A.S. Pozharskiy, A.K. Taskuzhina. Patent for the invention No. 36219 (2023) «Set of highly specific oligonucleotides for the detection of five potato viruses in plant material».

 

Contact information:

 87273947768 — Gritsenko Dilyara Alexandrovna, Head of the Laboratory d.kopytina@gmail.com

+77477237558 — G. K. Nizamdinova., senior researcher nizamdin13@gmail.com