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HomeAP 22787867 (superviser Kumarbayeva M.)

AP 22787867 (superviser Kumarbayeva M.)

Brief description of the project

(2024-2026)

 Project title: IRN AP22787867 «Molecular screening and identification of isolates insensitive to strobulirin fungicides with Quinone Outside Inhibitor Qol in wheat Pyrenophora tritici-repentis population».

 Relevance.  Tan spot of wheat, the causative agent of which is Pyrenophora tritict-repentis (Ptr), is the most aggressive among leaf diseases of wheat both worldwide and in Kazakhstan. Under favorable conditions for the development of the disease, crop losses reach 50-65%. The increased importance of tan spot of wheat in recent years is due to an increase in wheat acreage with a combination of direct sowing (no-till) and monoculture; the absence of wheat varieties resistant to diseases and the emergence of new Ptr races; using ineffective fungicides, which ensured the geographical spread of the pathogen over large areas. In this situation, the use of fungicides has become one of the most important tools to help reduce crop losses. Strobilurine fungicides with an external quinone inhibitor, Qol, are widely used against tan spot, but there are insufficient studies reporting the sensitivity of the most aggressive Ptr isolates to fungicides. In Kazakhstan, studies aimed at studying the sensitivity of Ptr isolates to fungicides have not been conducted before. The widespread use of strobilurin fungicides against leaf blotches requires a study of its mechanism, i.e. there is a need to understand the nature of resistance to strobulirin fungicides. In this regard, the molecular screening and identification of isolates of Qol insensitive to strobulirin fungicides in the wheat Pyrenophora tritici-repentis population is an urgent problem.

 The goal of the project: Molecular screening and identification of isolates insensitive to strobulirin fungicides with Quinone outside inhibitor Qol in wheat Pyrenophora tritici-repentis population

 Expected results:

  1. Identification of the causative agent of tan spot -on wheat in Almaty, Kostanay and Akmola regions. Creation of a collection of monoconidial isolates of the causative agent of tan spot P. tritici-repentis.
  2. Molecular screening of P. tritici-repentis monoconidial isolates for the presence of CHS-1 chitin synthase genes, ITS internal transcribed spacer, and ToxA and ToxB toxin formation genes.
  3. To study the effects of fungicides with an external quinone inhibitor Qol on the resistance of wheat genotypes, depending on the presence of Tsn1 and Tsc2 tan spot resistance genes in them.

 Scientific Supervisor of the project: PhD, Senior Researcher at the IPBB, Kumarbayeva Madina.

 Research group: 

  1. Keishilov Zh.S.-researcher
  2. Aydana Kharipzhanova – PhD, Junior researcher
  3. Bolatbekova A. A.-PhD doctoral student,
  4. Zhanuzak D. Junior researcher
  5. Bakhytuly K. Junior researcher

 List of publications of the project’s participants (2018-2023)

  1. Kumarbayeva M., Kokhmetova A., Kovalenko N., Atishova M., Keishilov Zh., Aitymbetova K. Characterization of Pyrenophora tritici-repentis(tan spot of wheat) Races in Kazakhstan // Phytopathologia Mediterranea. –  – Vol. 6. – P. 243-257. IF – 2.08. WOS Q2. Scopus: percentile 65.
  2. Kokhmetova A., Rathan N.D., Sehgal D., Malysheva A., Kumarbayeva M., Atishova M., Bolatbekova A., Krishnappa G., Gultyaeva E., Kokhmetova A., Keishilov Zh., Bakhytuly K. QTL mapping for seedling and adult plant resistance to stripe and leaf rust in two winter wheat populations // Frontiers in Genetics. – 2023. – Vol. 14. DOI: 10.3389/fgene.2023.1265859. IF – 3.7. WOS Q2. Scopus: percentile 53.
  3. Malysheva A., Kokhmetova A., Urazaliev R., Kumarbayeva M., Keishilov Z., Nurzhuma M., Bolatbekova A., Kokhmetova A. Phenotyping and Identification of Molecular Markers Associated with Leaf Rust Resistance in the Wheat Germplasm from Kazakhstan, CIMMYT and ICARDA // Plants. – 2023. – Vol. 12(15). – P. 2786. DOI: 10.3390/plants12152786. IF – 4.5. WOS Scopus: percentile 83.
  4. Kokhmetova A.M, Rsaliyev S., Atishova M.M, Kumarbayeva M.T, Malysheva A., Keishilov Z., Zhanuzak D., Bolatbekova A. Evaluation of wheat germplasm forresistanceto leaf rust (Puccinia triticina) and identificationofthe sources of Lr resistance genes using molecular markers // Plants. – 2021. – Vol. 10(7). – P. 1484. DOI: 10.3390/plants10071484. IF – 3.935. WOS Scopus: percentile 56.
  5. Kokhmetova A., Kumarbayeva M., Atishova M. Identification of high-yielding wheat genotypes resistant to Pyrenophora tritici-repentis (tan spot) // Euphytica. – 2021. – Vol. 217. – P. 97. DOI:10.1007/s10681-021-02822-y IF 1.84.WOS Q2. Scopus: percentile 74.
  6. Kokhmetova A.M, Rsaliyev A., Malysheva A.A, Atishova M., Kumarbayeva M.T, Keishilov Z. Identification of StripeRust Resistance Genes inCommon WheatCultivars and BreedingLines from Kazakhstan // Plants. – 2021. – Vol. 10(11). – P. 2303. DOI: 3390/plants10112303 IF –3.935. WOS Q1. Scopus: percentile 56.
  7. Kokhmetova A., Sehgal D., Ali S., Atishova M., Kumatbayeva M., Leonova I., Dreisigacker S. Genome-wide association study of tan spot resistance in a hexaploid wheat collection from Kazakhstan // Frontiers in Genetics. – 2021. – Vol. 11. – P. 581214. IF – 4.7. WOS Q2. Scopus: percentile 53.
  8. Kumarbayeva M.T., Kovalenko N.M., Kremneva O.Yu., Atishova M.N., Keishilov Zh.S., Malysheva A.A., Zhanuzak D.K., Bolatbekova A.A., Kokhmetova A.M. Identification of Wheat Samples for Resistance to Toxins of Pyrenophora Tritici-Repentis (Ptr) // International Journal of Biology and Chemistry. – 2022. – Vol. 15(1). – P. 64-72. DOI: 10.26577/ijbch.2022.v15.i1.07. IF – 0.3. WOS Q4.
  9. Malysheva A.A., Kokhmetova A.M., Kumarbayeva M.T., Zhanuzak D.K., Bolatbekova A.A., Keishilov Zh.S., Gultyaeva E.I., Kokhmetova A.M., Tsygankov V., Dutbayev Y.B., Dubekova S.B. Identification of Carriers of Puccinia Striiformis Resistance Genes in the Population of Recombinant Inbred Wheat Lines // International Journal of Biology and Chemistry. – – Vol. 15(1). – P. 4-10. DOI: 10.26577/ijbch.2022.v15.i1.01. IF – 0.3. WOS Q4.
  10. Kokhmetova A., Kovalenko N.V., Kumarbayeva M. Pyrenophora tritici-repentis population structure in the Republic of Kazakhstan and identification of wheat germplasm resistant to tan spot // Вавиловский журнал генетики и селекции. – 2020. – Vol. 24. – P. 722-729. IF – 0.9. WOS Q3. Scopus: percentile
  11. 6Kokhmetova A., Atishova M., Kumarbayeva M., Leonova I. Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot // Вавиловский журнал генетики и селекции. – 2019. – Vol. 23(7). – P. 879-886. DOI:10.18699/VJ19.562. IF – 0.9. WOS Q4. Scopus: percentile
  12. Кохметова А.М., Кейшилов Ж.С., Ғалымбек Қ., Кумарбаева М.Т. Қазақстанда өсірілетін бидай сорттарының пиренофороз Pyrenophora tritici-repentis ауруына төзімділігіне фитопатологиялық скрининг жүргізу // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2019. – №2. – С.213-218.
  13. Кумарбаева М.Т., Кохметова А.М., Ғалымбек К., Кейшилов Ж.С., Рсалиев А.С. Генетико-селекционное и фитопатологическое изучение устойчивости к пиренофорозу образцов пшеницы. Вестник Науки Казахского Агротехнического Университета. №3(102) 2019. С. 47-57.
  14. Кохметова. А.М., Кеишилов. Ж.С., Ғалымбек. Қ., Кумарбаева М.Т. Қазақстанда өсірілетін бидай сорттарының пиренофороз Pyrenophora tritici-repentis ауруына төзімділігіне фитопатологиялық скрининг жүргізу // Ізденістер, нəтижелер – Исследования, результаты – 2019. № 2 (82). — С.213-218.
  15. Кейшилов Ж.С., Кохметова А.М., Маденова А.К., Кумарбаева М.Т., Жигитбекова А.Д. Күздік бидай коллекциясының пиренофорозға (Pyrenophora tritici-repentis) төзімділігін бағалау // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2020. – №2. –Б.128-135.
  16. Кумарбаева М.Т. Кохметова А.М., Рсалиев А.С. Фитопатологический скрининг на устойчивость к пиренофорозу и оценка хозяйственно-ценных признаков образцов озимой мягкой пшеницы // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2020. – №2. –С.247-252.
  17. Kumarbaeva M.T., Kokhmetova A.M., Keishilov Zh.S., Chudinov V., Zhanuzak D.K. 2022. Disease monitoring to determine the level of spread and development of the pathogen pyrenophora tritici-repentis in Kazakhstan // Herald of science of S. Seifullin KazATU. 1(112): 906. DOI:10.51452/kazatu.2022.1(112).906
  18. Keishilov Zh.S., Kokhmetova A.M., Kumarbaeva M.T., Bolatbekova A.A., Malysheva A.A., Kokhmetova A.M. 2022. Monitoring of leaf rust (puccinia recondita) of spring wheat in northern Kazakhstan 2019-2021 // Herald of science of S. Seifullin KazATU. 1(112): 930. DOI: 10.51452/kazatu.2022.1(112).930
  19. Kumarbayeva, M.K., A.M. Kokhmetova, N.M. Kovalenko, O.Yu. Kremneva, M.N. Atishova, Zh.S. Keishilov, A.A. Malysheva, D.K. Zhanuzak, A.A. Bolatbekova, and A.M. Kokhmetova. “Identification of Wheat Samples for Resistance to Toxins of Pyrenophora Tritici-Repentis (Ptr)”. International Journal of Biology and Chemistry 15 (1):64-72. https://doi.org/10.26577/ijbch.2022.v15.i1.07.
  20. Malysheva, A.A., A.M. Kokhmetova, M.K. Kumarbayeva, D.K. Zhanuzak, A.A. Bolatbekova, Zh.S. Keishilov, E.I. Gultyaeva, A.M. Kokhmetova, V. Tsygankov, Y.B. Dutbayev, and S.B. Dubekova. 2022. “Identification of Carriers of Puccinia Striiformis Resistance Genes in the Population of Recombinant Inbred Wheat Lines”. International Journal of Biology and Chemistry 15 (1):4-10. https://doi.org/10.26577/ijbch.2022.v15.i1.01.
  21. Kokhmetova, A., Malysheva, A., Kumarbayeva, M., Bolatbekova, A., & Kokhmetova, A. 2022. Evaluation of the wheat recombinant inbred lines for resistance to leaf rust. Научный журнал «Доклады НАН РК», (2), 48–60. https://doi.org/10.32014/2022.2518-1483.147
  22. Кеишилов, Ж.С., Кохметова, А.М., Кумарбаева, М.Т., Жанузақ, Д.К., Рсалиев, Ш.С. Бидайдың сары тат (Puccinia striiformis f.sp. tritici) ауруына Алматы облысы бойынша 2019–2021 жылдары жүргізілген мониторингі // Вестник Карагандинского университета. Серия «Биология. Медицина. География». – 2022. – Vol. 2. – №. 106. – P. 82-88. DOI31489/2022BMG2/82-88
  23. Кумарбаева М.Т., Кохметова А.М., Кеишилов Ж.С., Малышева А.А., Болатбекова А.А. Идентификация источников устойчивости к желтой ржавчине (Puccinia striiformis westend f. sp. tritici) пшеницы в коллекции озимых образцов //– Ізденістер, нәтижелер – Исследования, результаты. – 2023. – №2 (98). – С. 89-101. DOI https://doi.org/10.37884/2-2023/09
  24. Кеишилов Ж.С., Кохметова А.М., Кумарбаева М.Т., Малышева А.А., Бахытұлы Қ. Бидайдың септориоз (Septoria tritici) ауруына алматы облысы бойынша 2022 жылы жүргізілген мониторингі //– Ізденістер, нәтижелер – Исследования, результаты. – 2023. – №2 (98). – С. 225-235. DOI https://doi.org/10.37884/2-2023/22
  25. Малышева А.А., Кохметова А.М., Кумарбаева М.Т., Кейшилов Ж.С., Дубекова С. Молекулярно-генетический скрининг Lr-генов устойчивости у образцов мягкой яровой пшеницы в условиях юговостока Казахстана // Ізденістер, нәтижелер – Исследования, результаты. – 2023. – №3 (99). – С. 92-99. (КОКСНВО).
  26. Жанузак Д.К., Кумарбаева М.Т., Болатбекова А., Бахытулы К., Кохметова А.М. Идентификация носителей генов устойчивости к желтой ржавчине на основе молекулярного скрининга образцов яровой пшеницы // Ізденістер, нәтижелер – Исследования, результаты. – 2023. – №3 (99). – С. 136-145. (КОКСНВО).
  27. Кumarbayeva М.Т., Кokhmetova А.М., Мalysheva A.A., Bolatbekova А.А., Кokhmetova А.М. Identification of sources of resistance to Septoria tritici in winter wheat germoplasm // Ізденістер, нәтижелер-Исследования, результаты. – 2023. – №4(100). – P. 57-62 (КОКСНВО).
  28. Кеишилов Ж.С., Кохметова А.М., Кумарбаева М.Т., Дутбаев Е.Б., Харіпжанова А.І., Дабабат А.А. Жаздық жұмсақ бидай үлгілерінің puccinia recondita және bipolaris sorokiniana ауру қоздырғыштарына төзімділігін фитопотологиялық бағалау // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2024. № 2 – 122-134 бет. (ККСОН МОН РК). DOI: https://doi.org/10.37884/2-2024/12
  29. Кеишилов Ж.С., Кохметова А.М., Кумарбаева М.Т., Болатбекова А.А.,Нұржұма М.Н. Қазақстанның солтүстік және оңтүстік облыстары бойынша, бидайдың пиренофороз (pyrenophora tritici-repentis) ауруына жүргізілген мониторингі // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2024. № 2 – 74-84 бет. (ККСОН МОН РК). DOI: https://doi.org/10.37884/2-2024/08
  30. Бахытулы К., Кохметова А.М., Умирзакова А.Т., Кулиев А.С., Кумарбаева М.Т., Кеишилов Ж.С. (2024). Мониторинграспространения Juglans regia L. в южных и юго-восточных регионах Казахстана и морфологическое изучение плодов грецкого ореха // Ізденістер нәтижелер-Исследования, результаты. – №4. – 2024.

Results for 2024:

  1. Monitoring of the spread and development of tan spot in the main grain-growing regions of the country (in Almaty, Turkestan, Kostanay and Akmola regions) was carried out in order to identify the causative agent of tan spot. Infectious material was collected and monoconidial isolates of P. tritici-repentis were isolated in pure culture. A collection of monoconidial isolates of Ptr was created, the racial composition was characterized and the virulence of the isolates was determined using Canadian differentiating varieties.
  2. Phytopathological screening and phenological observations of a collection of wheat samples at the stage of an adult plant were carried out in the field conditions of the Almaty region. Phenological observations and evaluation of wheat lines for the main elements of productivity were carried out.
  3. Isolation of monoconidial isolates of P. tritici-repentis (Ptr) in pure culture and morphological identification of the isolates were carried out. As part of the study, the isolation of monoconidial isolates of Ptr into pure culture was carried out. A collection of 107 pure cultures of monoconidial isolates of P. tritici-repentis was created for further studies, including pathogenicity tests and molecular characterization.

Publications (2024):

  1. Кеишилов Ж.С., Кохметова А.М., Кумарбаева М.Т., Болатбекова А.А.,Нұржұма М.Н. Қазақстанның солтүстік және оңтүстік облыстары бойынша, бидайдың пиренофороз (pyrenophora tritici-repentis) ауруына жүргізілген мониторингі // «Ізденістер, нәтижелер-Исследования, результаты», –Алматы, 2024. № 2 – 74-84 бет. (ККСОН МОН РК). DOI: https://doi.org/10.37884/2-2024/08 КОКНВО
  2. Kumarbayeva М, Kokhmetova A., Keishilov Zh. Identification of promising wheat lines resistant to tan spot (Pyrenophora tritici-repentis) using breeding and molecular methods. ICPBB 2024, Р. 67. (Almaty, Kazakhstan). тезис