AP 23490217 (superviser Anuarbek S.)

Results for 2025

  1. Botanical expedition routes were developed to collect Aegilops L. species (Ae. cylindrica, Ae. crassa, Ae. tauschii, Ae. triuncialis, and Ae. juvenalis) growing in southeastern Kazakhstan. As a result of expeditions conducted in 2025, nine populations of Ae. cylindrica and four populations of Ae. triuncialis were collected.
  2. Phenotypic and phenological assessments were conducted on 29 Aegilops accessions grown in the Almaty region, including four species native to Kazakhstan.
  3. DNA was isolated from two Aegilops species using the CTAB (cetyltrimethylammonium bromide) method in triplicate for subsequent genetic analysis using SSR and ITS markers.
  4. Optimization of polymerase chain reaction (PCR) conditions for 20 wheat SSR markers localized on chromosomes 1D –7D has been completed. The optimized parameters will be applied in further genotyping of Aegilops species.
  5. Work has begun on genotyping two Aegilops species – Ae. cylindrica and Ae. triuncialis and determining the nucleotide sequences of their chloroplast genomes.

Information for potential users

The results of the completed research are of high scientific and practical value, as they contribute to expanding knowledge about the biological diversity of the genus Aegilops L. in Kazakhstan. Organized expeditions allowed us to collect new populations of the species Ae. cylindrica and Ae. triuncialis, expanding our collection of genetic material and creating a basis for further breeding and genetic research. Phenotypic and phenological assessment of 29 Aegilops L. species provides insight into their adaptive and morphological characteristics in the Almaty region, while DNA extraction and the use of SSR and ITS markers create opportunities for studying the genetic diversity and relationships between species. The data obtained make it possible to identify valuable genetic resources that can be used in wheat breeding to enhance resistance to stress conditions and improve crop productivity. Overall, the research results contribute to the conservation, assessment, and use of the genetic diversity of the genus Aegilops in Kazakhstan and beyond.

Publications 2025

Abstracts of the international conference (indicating the form of the report )

  1. Amalova A., Yessimbekova M, Turemuratova S., Gulin A., Anuarbek Sh.  Study of the Aegilops L. species of Kazakhstan // Материалы «3-ий Международный симпозиум по передовым исследованиям по сохранению разнообразия растений в третьем полюсе мира (PDCR 2025). – Алматы. – 2025. – С.47 (постер)