Brief description of the project
(2025-2027)
Project title: IRN AP26102771 «Generation of Promising Lines for the Breeding of Spring Rapeseed Using Omics Technologies».
Relevance.
The development of new rapeseed varieties and lines, as well as the improvement of existing ones, is one of the main priorities of breeding and seed production in Kazakhstan. Traditionally, the creation of new rapeseed varieties has been based on classical breeding methods, whereas in developed countries modern biotechnological and omics technologies have been actively integrated into breeding programs for many years. This project is aimed at developing promising spring rapeseed lines using modern technologies, which will enhance breeding efficiency and increase yield as well as seed oil quality.
The relevance of this research is driven by the need to develop new, well-adapted, and competitive rapeseed varieties in response to climate change and the increasing demand for food quality.
The goal of this project is to study a rapeseed collection consisting of intervarietal, interspecific hybrids, mutants, and their doubled haploids using modern omics approaches to develop new promising rapeseed breeding material. The project will focus on the investigation of quantitative traits related to yield and qualitative traits associated with the fatty acid composition of seeds.
This project adopts a multidisciplinary approach, integrating methods of biotechnology, genetics, breeding (both conventional and molecular), and biochemistry.
The implementation of this project will create conditions for the efficient development of promising rapeseed lines and their hybrids, covering a wide range of qualitative and quantitative characteristics, as well as traits related to stress tolerance under environmental conditions.
The goal of the project: To study collection samples of spring rapeseed using traditional and omics breeding technologies.
Expected results:
1. The existing rapeseed collection (at least 100 lines), consisting of intervarietal and interspecific hybrids, mutants, and doubled haploids, will be evaluated and characterized.
2. At least 15 lines from the rapeseed collection will be studied for drought tolerance during the seed germination stage, and transcriptomic analysis followed by RNA sequencing will be conducted under stress and control conditions. In addition, at least 15 rapeseed lines will be evaluated for drought tolerance during the budding and flowering stages, with transcriptomic analysis and subsequent RNA sequencing performed under stress and control conditions. The expression of rapeseed genes associated with drought tolerance will be determined.
3. Metabolomic analysis of the rapeseed collection samples will be carried out to assess quality traits and stress tolerance. Homozygosity of the rapeseed collection samples will be evaluated using FISH and GISH methods. Data obtained during the study of the rapeseed collection will be analyzed, and parental lines will be selected for further breeding programs.
Scientific Supervisor of the project:
Sapakhova Zagipa Beisenovna, PhD, Associate Professor, Leading Researcher. H-index: 8 according to the Scopus
(https://www.scopus.com/authid/detail.uri?authorId=56046815800&origin=resultslist)
and Web of Science (WoS) databases
(https://www.webofscience.com/wos/author/record/156901).
Research group:
Shamekova Malika Khabidulaevna, PhD, Professor, Head of the Laboratory of Breeding and Biotechnology. H-index: 8 according to the Scopus
(https://www.scopus.com/authid/detail.uri?authorId=55617198500)
and Web of Science (WoS) databases
(https://www.webofscience.com/wos/author/record/98695).
Daurova Ainash Kenenbaevna, MSc, PhD student, Senior Researcher. H-index: 6 according to the Scopus
(https://www.scopus.com/authid/detail.uri?authorId=57201673636&origin=resultslist)
and Web of Science (WoS) databases
(https://www.webofscience.com/wos/author/record/2024273).
Volkov Dmitriy Vladimirovich, MSc, PhD student, Senior Researcher. H-index: 4 according to the Scopus
(https://www.scopus.com/authid/detail.uri?authorId=57218835515&origin=resultslist)
and Web of Science (WoS) database
(https://www.webofscience.com/wos/author/record/636236).
Akbaev Asset Maulenovich, MSc, Junior Researcher.
Sadykova Assel Dulatovna, Bachelor’s degree, Laboratory Assistant.
List of publications of the project’s participants
- Daurov D., Daurova A., Sapakhova Z., Kanat R., Akhmetzhanova D., Abilda Z., Toishimanov M., Raissova N., Otynshiyev M., Zhambakin K., Shamekova M. The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers. Agronomy. 2024, 14, 1782. https://doi.org/10.3390/agronomy14081782
- Sapakhova, Z.; Abilda, Z.; Toishimanov, M.; Daurov, D.; Daurova, A.; Raissova, N.; Sidorik, A.; Kanat, R.; Zhambakin, K.; Shamekova, M. Early Generation Selection of Potato Breeding Lines. Horticulturae 2024, 10, 1121. https://doi.org/10.3390/horticulturae10101121
- Sapakhova Z., Islam K.R., Toishimanov M., Zhapar K., Daurov D., Daurova A., Raissova N., Kanat R., Shamekova M., Zhambakin K. Mulching to improve sweet potato production. Journal of Agriculture and Food Research, 2024, 15, 101011. https://doi.org/10.1016/j.jafr.2024.101011
- Gritsenko D, Daurova A, Pozharskiy A, Nizamdinova G, Khusnitdinova M, Sapakhova Z, Daurov D, Zhapar K, Shamekova M, Kalendar R, Zhambakin K. Investigation of mutation load and rate in androgenic mutant lines of rapeseed in early generations evaluated by high-density SNP genotyping. Heliyon. 2023 Mar 2;9(3):e14065. doi: 10.1016/j.heliyon.2023.e14065. (IF-3.7, Q2, WoS; Процентиль – 86, Scopus).
- Daurova A.K., Volkov D.V., Daurov D.L., Zhapar K.K., Sapakhova Z.B., Gritsenko D.A., Pozharskiy A., Abdrakhmanova A., Shamekova M.Kh., Zhambakin K.Zh. Production mutant lines of turnip rape (Brassica rapa) and its interspecific hybrids in the isolated microspore culture // Experimental Biology. – 2022. – Vol. 2 (91). – P:100-108pp. https://doi.org/10.26577/eb.2022.v91.i2.08
- Daurova A.K., Volkov D.V., Daurov D.L., Zhapar K.K., Sapakhova Z.B., Shamekova M.Kh., Zhambakin K.Zh. Improvement of Breeding-valuable Traits of Rapeseed (Brassica napus) using Mutagenesis // International Journal of Agriculture and Biology. – Intl. J. Agric. Biol. – Vol. 28. 2022. – P.219‒227. IF (WoS) -0.9. Q-3; Процентиль (Scopus) – 53.
- Daurova Ainash, Daurov Dias, Volkov Dmitriy, Aibek Karimov, Zhandos Abai, Daniyar Raimbek,Kuanish Zhapar, Kabyl Zhambakin, Malika Shamekova. Mutagenic treatment of microspore-derived embryos of turnip rape (Brassica rapa) to increase oleic acid content. Plant Breeding. 2020; IF (Scopus) – 3, процентиль — 71, Квартиль (WoS) — Q3, Индекс цитирования – 2, https://doi.org/10.1111/pbr.12830
- Daurova A., Daurov D., Volkov D.,Zhapar K.,Raimbek D.,Shamekova M.,Zhambakin K. Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits. OCL — Oilseeds and fats, Crops and Lipids, 2020, 27, 27 процентиль — 70, Индекс цитирования – 2, DOI:10.1051/ocl/2020041
- Sapakhova, Z.; Abilda, Z.; Toishimanov, M.; Daurov, D.; Daurova, A.; Raissova, N.; Sidorik, A.; Kanat, R.; Zhambakin, K.; Shamekova, M. Early Generation Selection of Potato Breeding Lines. Horticulturae 2024, 10, 1121. https://doi.org/10.3390/horticulturae10101121
- Kanat, R.; Shamekova, M.; Sapakhova, Z.; Toishimanov, M.; Daurov, D.; Raissova, N.; Abilda, Z.; Daurova, A.; Zhambakin, K. Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development. Biology 2024, 13, 857. https://doi.org/10.3390/biology13110857
Information on existing patents and other intellectual property documents:
1 Patent №244781 for a breeding achievement for the spring rapeseed variety “Tengri”. Authors: Zhambakin K.Zh., Daurova A.K., Shamekova M.H., Sapakhova Z.B., Daurov D.L., Oshergina I.P., Ten E.A., Kradetskaya O.O. Date of patent: 03.11.2024.
2 Computer Program: Digital seed quality control platform “E-Tukym”. Authors: Raisova N.U., Zhambakin K.Zh., Sapakhova Z.B., Kurdyumov A.A., Daurov D.L., Daurova A.K., Toyshimanov M.R., Shamekova M.H. Certificate of registration in the State Register of copyright-protected objects № 45900, issued on 16.05.2024..
Results for 2025:
Rapeseed lines exceeding the standard variety Maiqudyk (40.73 c/ha) in yield were identified. The most productive lines were: DHK12-4 -54.55 c/ha; DHG-5 — 50.0 c/ha; DHK12-1 — 49.55 c/ha; DHGK-1 — 43.64 c/ha; DHZ 4-3-20 — 45.0 c/ha.
Seed oil content ranged from 46.08 to 50.33%, and protein content ranged from 23.08 to 26.55%. Lines distinguished by high oil content included DHK12-4 (50.33%), DHZ 4-1-20 (49.54%), DHGK-1 (49.27%), and the variety Zolotistaya (49.55%). The highest protein content was observed in DHivH-1 (26.55%), DHG-5 (25.77%), and Zolotistaya (25.26%).
These results indicate high plasticity and adaptability of the studied lines and confirm the effectiveness of a comprehensive approach combining classical breeding methods with modern biotechnological technologies.
During genotyping of the rapeseed collection, 65 molecular markers were identified, associated with traits such as the number of pods per plant, seeds per pod, 1000-seed weight, and seed color. Genotyping of 50 lines using these markers revealed associations of three SSR markers (BrGMS2864, BrEMS0015, BrGMS4031) with key agronomic traits, including pod length and seed weight.
Publications (2025):
