Kapytina A, Kolchenko M, Kerimbek N, Pozharskiy AS, Nizamdinova G, Taskuzhina A, Adilbayeva K, Khusnitdinova M, Amidullayeva M, Moisseyev R, et al. Distribution of Wheat-Infecting Viruses and Genetic Variability of Wheat Streak Mosaic Virus and Barley Stripe Mosaic Virus in Kazakhstan. Viruses. 2024; 16(1):96. https://doi.org/10.3390/v16010096
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GritsenkoDilyara Alexandrovna
Head of the Laboratory, PhDHead of laboratory: biography
Head of the laboratory since 2019. Bachelor’s degree in Engineering and Technology from KazNU named after al-Farabi (2006-2010). Master’s degree in Natural Sciences from KazNU named after al-Farabi (2010-2012). PhD in 2019 with a thesis on «Creation of a viral vector for the production of recombinant proteins in plants and the study of their expression efficiency». Internship at the Tolkovsky Laboratory at the Volcani Center Plant Protection Institute in Israel. Awarded the «Nazarbayev Scholarship» by the President for academic excellence and high achievements in research in 2011. Author of over 50 scientific works, Hirsch index – 5.
LABORATORY TEAM
Dilyara Gritsenko, PhD (Head of the Laboratory)
G.K. Nizamdinova, PhD (Senior Researcher)
M.A. Khusnitdinova, PhD (Senior Researcher)
A.S. Pozharskiy (Researcher)
A.I. Kapytina (Junior Researcher)
N.M. Kerimbek (Junior Researcher)
A.K. Taskuzhina (Junior Researcher)
V.S. Kostyukova (Junior Researcher)
R.T. Kenzhebekova (Junior Researcher)
M.V. Kolchenko (Laboratory Assistant)
K.S. Adilbayeva (Laboratory Assistant)
R.M. Moiseev (Laboratory Assistant)
A.Mendybayeva (Laboratory Assistant)
A.N. Makhambetov (Laboratory Assistant)
Z.K. Dairbekova (Laboratory Assistant)
M.I. Yelchaninov (Laboratory Assistant)
MISSION OF THE LABORATORY
Ensuring the country’s food security through fundamental and applied research in plant breeding, rational use of genetic resources, and plant protection.
Developing modern technologies and knowledge for protecting strategically important agricultural crops and applied research in diagnostics systems, detection, and identification of economically important phytopathogens, quarantine pests and pathogens, and genetic passportization of agricultural crops. 
MAIN RESEARCH FOCUS OF THE LABORATORY
01Identification and molecular-genetic research of phytopathogens and pests.
02Development of recombinant molecules for biotechnology.
03Whole-genome studies of pathogens and pests of plants.
04Study of phytopathological and genetic aspects of populations of wild and cultivated apple.
05Genetic potential of potato resistance to major diseases and pests.
06Study of the genetic diversity of the local breeding collection of tomato varieties.
07Study of the genetic potential of sugar beet and its phytopathological status.
08Collaboration and services in molecular genetic research.
MAIN RESULTS OF THE ACTIVITIES
Established a system for regenerating various grape varieties from meristematic and apical tissue.
Developed a system for simultaneous diagnostics of six grape viruses (ArMV GFkV GFLV GVA GLRV-1 GLRV-3).
Developed a system for simultaneous diagnostics of five apple viruses (ASGV ASPV ACLSV ApMV ToRSV).
Conducted an analysis of the genetic diversity of wild apple populations in Kazakhstan using DNA markers.
Genotyped technical and table grape varieties of domestic and foreign selection cultivated in Kazakhstan.
Developed viral vectors based on the genome of grapevine virus A for the expression of heterologous proteins in plants.
Developed a highly sensitive diagnostics system for Erwinia amylovora (bacterial fire blight of fruit and berry crops) based on the loop-mediated isothermal amplification of nucleic acids method.
Established a GMO detection system.
21 publications
KEY PUBLICATIONS (2019-2024)
Gritsenko D. et al. First evaluation of genetic diversity among honeybee populations in Kazakhstan // Apidologie. 2023. 54, № 6. P. 61.
Khusnitdinova M. et al. Problems and prospects of sugar beet cultivation in Kazakhstan (Changed title according to reviewers’ comments) // Agronomy Research. 2023. Vol. 21.
Kairova G. et al. Evaluation of Fire Blight Resistance of Eleven Apple Rootstocks Grown in Kazakhstani Fields // Applied Sciences. 2023. Vol. 13, № 20. P. 11530.
Kolchenko M. et al. Wild Malus niedzwetzkyana Dieck ex Koehne as a Genetic Resource for Fire Blight Resistance // Horticulturae. 2023. Vol. 9, № 10. P. 1066.
Pozharskiy A. et al. Genetic structure and genome-wide association study of the traditional Kazakh horses // animal. 2023. P. 100926.
Kolchenko M. et al. Genetic Characterization of Raspberry Bushy Dwarf Virus Isolated from Red Raspberry in Kazakhstan: 4 // Viruses. Multidisciplinary Digital Publishing Institute, 2023. Vol. 15, № 4. P. 975.
Gritsenko D. et al. Investigation of mutation load and rate in androgenic mutant lines of rapeseed in early generations evaluated by high-density SNP genotyping // Heliyon. Elsevier, 2023. Vol. 9, № 3.
Pozharskiy A. et al. Genetic diversity of the breeding collection of tomato varieties in Kazakhstan assessed using SSR, SCAR and CAPS markers // PeerJ. 2023. Vol. 11. P. e15683.
Kairova G, Daulet N, Solomadin M, Sandybayev N, Orkara S, Beloussov V, Kerimbek N, Gritsenko D, Sapakhova Z. Identification of Apple Varieties Resistant to Fire Blight (Erwinia amylovora) Using Molecular Markers. Horticulturae. 2023; 9(9):1000. https://doi.org/10.3390/horticulturae9091000
Patents
2017D.A. Gritsenko, I.S. Gritsenko. Copyright certificate for the computer program DNARNAref, IS 009529, No. 1890 (2017), MYURK.Patent
2018A.O. Sagitov, B.A. Duisembekov, A.B. Zhanarbekova, B.K. Kopzhasarov, G. Kaldybekkyzy, A.A. Jaimurzina, A.S. Dinasilov, Z.B. Beknazarova, A.M. Sarbasova, A.A. Darubaev, A.A. Seisenova, D.M. Dzhumakhan, T.S. Sambetova, D.A. Gritsenko. Copyright certificate «Applied scientific research in the field of protection and quarantine of plants to ensure phytosanitary security of the Republic of Kazakhstan (scientific work)», No. 0645 (2018).Patent
2019N.N. Galiakparov, M. Omashova, D.A. Gritsenko. Patent for the invention «Set of synthetic oligonucleotides for the detection of apple viruses by RT-PCR», No. 33634 (2019). (https://gosreestr.kazpatent.kz/Invention/DownLoadFilePdf?patentId=283865&lang=ru)Patent
2019N.N. Galiakparov, D.A. Gritsenko, N.N. Deryabina. Patent for the invention «Viral vector for transient expression of heterologous proteins in plants», 33632 (2019). (https://gosreestr.kazpatent.kz/Invention/DownLoadFilePdf?patentId=283863&lang=ru)Patent
2019N.N. Galiakparov, D.A. Gritsenko, N.N. Deryabina. Utility model patent No. 4583 (2019) «T-vector for cloning PCR products of different sizes». (https://gosreestr.kazpatent.kz/Utilitymodel/Details?docNumber=316755)Utility model patent
2020D.A. Gritsenko, N.N. Galiakparov. Utility model patent «Complex of antigens of influenza virus subtype H3N8», No. 5764 (2020).Utility model patent
2022D.A. Gritsenko, A.I. Kapytina, N. Kerimbek. Utility model patent No. 7018 (2022) «Set of synthetic oligonucleotides for the detection of raspberry ring spot virus».Utility model patent
2022D.A. Gritsenko, A.S. Pozharskiy, V.S. Kostyukova, K. Adilbayeva. Utility model patent No. 7296 (2022) «Set of synthetic oligonucleotides for the detection of tomato brown rugose fruit virus».Utility model patent
2023D.A. Gritsenko, A.S. Pozharskiy, A.K. Taskuzhina. Patent for the invention No. 36219 (2023) «Set of highly specific oligonucleotides for the detection of five potato viruses in plant material».Patent
SERVICES
Detection of viral pathogens:
Grapevine virus A
Grapevine short node virus
Grapevine leafroll-associated virus-1 and 3
Arabis mosaic virus
Grapevine fleck virus
Apple chlorotic leaf spot virus
Apple stem pitting virus
Apple stem grooving virus
Apple mosaic virus
Tomato ringspot virus
Tomato brown rugose fruit virus (ToBRFV)
Barley stripe mosaic virus
Wheat streak mosaic virus
Wheat mosaic emaravirus
Beet necrotic yellow vein virus
Raspberry leaf mottle virus (RLMV)
Raspberry leaf blotch virus (RLBV).
Detection of bacterial pathogens:
Bacterial fire blight of fruit crops, Brown rot of potato.
Detection of fungal pathogens:
Moniliosis of fruit crops.
Identification of insects using PCR:
Lymantria Dispar Dispar
Nematodes.
Contact information
87273947768 — Gritsenko Dilyara Alexandrovna, Head of the Laboratory d.kopytina@gmail.com
+77477237558 — G. K. Nizamdinova., senior researcher nizamdin13@gmail.com
