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AP 19676010 (supervizer Romadanova N.V.)

Brief description of the project

(2023-2025)

 

Project title: IRN АР19676010 «Development of biotechnology for ex situ conservation of rare, endangered Rosaceae species, for the restoration of natural populations».

 

Relevance. The aim of the project is to develop a biotechnology for the preservation in cryogenic bank and in vitro a germplasm of rare, endangered Rosaceae species, listed in the Red Book of Kazakhstan, the production of planting stocks for the restoration of natural populations.

The problem that the project is aimed at is that some Rosaceae species are endangered in Kazakhstan: Amygdalus ledebouriana Schlecht., Crataegus ambigua C.A. Mey. ex A. Beck, Cotoneaster karatavikus Pojark., Louiseania ulmifolia (Franch.) Pachom, Rosa pavlovii Chrshan., Sorbus persica Hedl., Malus sieversii (Ledeb.) M. Roem. and others. The main reason for the disappearance of plants is human economic activity. The death of plants in large quantities leads to their extinction and to global changes in ecosystems. The most vulnerable are rare species of flora – relics and endemics, the distribution of which depends on the boundaries of a particular area.

Many countries provide protection for endangered plants. The Red Book has been created in Kazakhstan, which is the main legislative document summarizing materials on the current state of rare and endangered species of plants and animals. In 2014, the second edited and supplemented edition of the Red Book of Plants was published, including 387 species, which is 81 species more than in the first edition of 1981. Replenishment of the list is the result of increasing human economic activity in recent years, as a result of which a further reduction of these species or even their disappearance is obvious.

Basic approaches to research. It is required to use scientific approaches to preserve the genetic material of endangered Rosaceae plants, including biotechnological methods, such as micropropagation, long-term storage in cryogenic banks, as well as restoration of natural populations by biotechnologically obtained plants.

Practical significance of research results. The studied plants have a valuable medicinal, food, decorative value, which are important objects for breeding, as well as sources of the gene pool. The preservation of these plants in vitro and in a cryobank, as well as the restoration of the ecology of their natural habitats, is of practical importance for solving urgent problems of the socio-economic, scientific and technical development of Kazakhstan. The planting stocks obtained during the implementation of the project will be in demand in breeding practice, and can also be sent to mass production for the implementation of the commercialization project. The created cryogenic collection and the in vitro collection can be used for the international exchange of genetic resources.

 

The goal of the project: is to develop a biotechnology for the preservation in cryogenic bank and in vitro a germplasm of rare, endangered Rosaceae species, listed in the Red Book of Kazakhstan, the production of planting stocks for the restoration of natural populations.

 

Expected results:

  1. Collection of plant material (annual shoots and fruits) of plants of the Rosaceae listed in the Red Book of Kazakhstan in their natural habitats. Studying the state of endangered populations using geobotanical methods. Selection of promising forms for breeding.
  2. Initiation to in vitro culture of shoots germinated from seeds and annual shoots. Diagnostics of test tube plants for the presence of endophytic contamination on a specialized medium 523 for the detection of fungi and bacteria. Optimization of nutrient media for micro propagation and for in vitro shoot rooting. Creation of an in vitro collection of aseptic plants as source material and medium-term storage of in vitro shoots at the for degrees of 4°C, long-term preservation of shoot tips at -196°C and planting stocks production.
  3. Optimization of methods for cryopreservation of shoot tips of some endangered Rosaceae species. Creation of a cryogenic seed bank, isolated embryonic axes, shoot tips and DNA at -196°C, long-term preservation of seeds in a freezer at a temperature of -20°C.

 

Scientific Supervisor of the project: Assoc. Prof., Ph.D. Leading research scientist of the Laboratory Germplasm Cryopreservation Romadanova N.V. More than 110 scientific papers have been published, of which 11 publications are included in the Web of Science database, 10 in the Scopus database. H-index 5. Scopus ID 36111616700; Web of Science ID BBD-5948-2021; ORCiD 0000-0003-1052-2753

 

Research group: 

1) Leading researcher, PhD, professor Kushnarenko S.V. More than 200 scientific papers have been published, of which 18 publications are included in the Web of Science database, 21 in the Scopus database. H-index 9. Scopus ID 6507153914; Web of Science ID M-6616-2015

2) Senior Researcher, PhD Aleksandrova A.M. 9 publications are included in the Web of Science database, H-index 3; Web of Science ID AGJ-1822-2022

3) Researcher Tolegen A.B. 12 scientific papers have been published, of which 2 publications are included in the Web of Science database, 2 in the Scopus database. H-index 2; Web of Science ID IPO-2780-2023; Scopus ID 57567940600.

4) Researcher Altayeva N.A. 54 scientific papers have been published, of which 4 publications are included in the Web of Science database, 3 in the Scopus database. H-index 3. Scopus ID 57216750088; Web of Science ID EKZ-9456-2022; ORCiD 0000-0002-5039-8999.

5) Junior researcher Zemtsova A.S. 5 scientific papers have been published.

6) Laboratory assistant Mikhailenko N.V. 2 scientific papers have been published

7) Laboratory assistant Artimovich N.A.

 

List of publications of the project’s participants (2018-2022)

List of main publications of the project leader included in the WoS database, Scopus and in the peer-reviewed journals of Kazakhstan Republic

  1. Kushnarenko S.V., Romadanova N.V., Aralbayeva M.M. Current state and in vitro conservation of the only endangered population of Corylus avellana in Kazakhstan // Res. on Crops. – 2020. – Vol. 21 (4). – P. 681-686. IF 0.413 DOI: 10.31830/2348-7542.2020.106 SCI 0, percentile 18.
  2. Romadanova N.V., Karasholakova L.N., Eshbakova K.A., Özek G., Özek T., Yur S., Kushnarenko S.V. Phytochemical analysis and antioxidant activity of Berberis iliensis M. Pop and Berberis integerrima Bunge fruits pulp // Res. on Crops. – 2021. – Vol. 22(4). – P. 940-947. IF 0.84 DOI: 10.31830/2348-7542.2021.154 SCI 0, percentile 38.
  3. Romadanova N.V., Tolegen A.B., Koken T.E., Nurmanov M.M., Kushnarenko S.V. Chemotherapy of in vitro apple shoots as a method of viruses eradication // International Journal of Biology and Chemistry. – 2021. – Vol. 14, № 1. – P. 48-55. WoSSCI 1. DOI: https://doi.org/10.26577/ijbch.2021.v14.i1.04
  4. 4. Romadanova N.V., Tolegen A.B., Kushnarenko S.V. Effect of Plant Preservative MixtureTM on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots // Plants. – 2022. – Vol. 11, Issue19, 2624. IF. 4.658, Q 1, percentile 97. SCI 1, WoSSCI 1. DOI: https://doi.org/10.3390/plants11192624
  5. Romadanova N.V., Kushnarenko S.V. Biotechnology for obtaining virus-free Malus sp. planting stocks // Bulletin of Karaganda University. Series “Biology. The medicine. Geography». – 2021. – No. 3 (103). – P. 102-118. DOI 10.31489/2021BMG3/102-118 (in Russian)
  6. Romadanova N.V., Aralbaeva M.M., Rymkhanova N.K., Baigaraev D.Sh., Ramazanov A.K., Ishmuratova M.Yu., Kushnarenko S.V. Cryopreservation as a way to increase laboratory germination and germination energy of seeds // Bulletin of Karaganda University. Series “Biology. The medicine. Geography». – 2022. – No 1 (105). – P. 86-95. DOI: 10.31489/2022BMG1/86-95 (in Russian)
  7. Kushnarenko S.V., Romadanova N.V., Turdiev T.T., Aralbaeva M.M., Kalybaev K.R. Preservation in a cryobank of Juglans regia L. accession from several populations in the Sairam-Ugam State National Natural Park // Bulletin of KazNU. Ecological Series». – 2022. – No 2 (71). – С 72-80. DOI: 10.26577/EJE.2022.v71.i2.07 (in Russian)

Publications of key staff of the research team:

  1. Alexandrova A.M., Karpova O.V., Nargilova R.M., Kryldakov R.V., Nizkorodova A.S., Zhigaylov A.V., Yekaterinskaya E.M., Kushnarenko S.V., Akbergenov R.Zh., Iskakov B.K. Distribution of potato (Solanum tuberosum) viruses in Kazakhstan // International Journal of Biology and Chemistry. – 2018. – 1 (11). – P. 33-40. DOI: https://doi.org/10.26577/ijbch-2018-1-311. WoSSCI 2, Q 4.
  2. Terletskaya N.V., Zorbekova A.N., Altayeva N.A., Bari G.T., Erezhetova U. Effect of drought on growth parameters and pigment complex of wheat lines obtained from interspecific crosses // Vestnik KazNU. Al-Farabi. Biological series. — №3 (76). — 2018. – p.130 – 139.
  3. Terletskaya N.V., Altayeva N.A., Erezhetova U. The influence of drought on the functioning of the photosynthetic apparatus of the flag leaf in alloplasmic lines obtained as a result of interspecific crossings of wheat // Bulletin of the L.N. Gumilev Eurasian National University. Biological Sciences Series — No. 4 (129). – 2019. – p.58-68.
  4. Karpova O., Alexandrova A., Yeriskina E., Kryldakov R., Gritsenko D., Galiakparov N., Iskakov B. Andean and Ordinary Strains of Potato Virus S Infecting Potatoes in Southern Kazakhstan // Plant Disease. – 2020. – 104 (2). – P. 599. DOI: 10.1094/pdis-09-19-1822-pdn. IF. 4.614. SCI 0, percentile 75; WoSSCI 0, Q 1.
  5. Terletskaya N.V., Altayeva N.A., Erezhetova U., Zorbekova A.N. Morphophysiological aspects of studying drought resistance of interspecific wheat hybrids // Bulletin of the Kazakh National University named after. al-Farabi, ser. environmental. — 2020. — No. 1 (62). — P.36-44.
  6. Terletskaya N.V., Shcherban A.B., Nesterov M.A., Perfil’ev R.N., Salina E.A., Altayeva N.A., Blavachinskaya I.V. Drought Stress Tolerance and Photosynthetic Activityof Alloplasmic Lines T. dicoccum x T. Aestivum. May 2020. International Journal of Molecular Sciences21(9):3356. DOI: 10.3390/ijms21093356. SCI 12, percentile 66; WoSSCI 9, Q 1
  7. Terletskaya N.V., Lee T.E., Altayeva N.A., Kudrina N.O., Blavachinskaya I.V., Erezhetova U. Some Mechanisms Modulating the Root Growth of Various Wheat Species under Osmotic-Stress Conditions. Plants 2020, 9, 1545; doi:10.3390/plants9111545. SCI 11, percentile 72; WoSSCI 10, Q 1.
  8. Kushnarenko S., Utegenova G., Danaeva G., Aralbaeva M. IoT-based fully automatic smart providing aseptic materials for in vitro and cryogenic collections of wild Juglans regia L. for genetic resources preservation // International Journal of Agricultural Resources, Governance and Ecology/ — 2021. – Vol. 17, Issue 2-4. – P. 238 – 246. DOI: 10.1504/IJARGE.2021.121663. IF 0.744. SCI 0, percentile 25; WoSSCI 0, Q 3.
  9. Karpova O., Alexandrova A., Nargilova R., Ramazanova M., Kryldakov R., Iskakov B. AtDREB2A gene expression under control of the inducible promoter and virus 5’-untranslated regions improves tolerance to salinity in Nicotiana tabacum // International Journal of Biology and Biomedical Engineering (by NAUN). – 2021. – 15. – P. 260-274. DOI: 10.46300/91011.2021.15.32. IF. 0.49. SCI 0, percentile 17.
  10. Kushnarenko S., Aralbayeva M., Rymkhanova N., Reed B.M. Initiation pretreatment with Plant Preservative MixtureTM increases the percentage of aseptic walnut shoots // In Vitro Cellular & Developmental Biology – Plant. – 2022. IF 2.347. SCI 1, percentile 74; WoSSCI 1, Q 2. https://doi.org/10.1007/s11627-022-10279-4
  11. Terletskaya N.V., Stupko V.Yu., Altayeva N.A., Kudrina N.O., Blavachinskaya I.V., Kurmanbayeva M.S., Erezhetova U. Photosynthetic activity of Triticum dicoccum × Triticum aestivum alloplasmic lines during vegetation in connection with productivitytraits under varying moister conditions. Photosynthetica 59 (1): 74-83, 2021. DOI 10.32615/ps.2021.003. SCI 3, percentile 49; WoSSCI 3, Q 2.
  12. Alexandrova A., Karpova O., Kryldakov R., Golyaev V., Nargilova R., Iskakov B., Pooggin M.M. Virus elimination from naturally infected field cultivars of potato (Solanum tuberosum) by transgenic RNA interference // International Journal of Molecular Sciences. – 2022. – Vol. 23(14). DOI: 10.3390/ijms23148020. IF. 5.542. SCI 0, percentile 85; Q 2.

Patents:

  1. Pat. 2019/0952. The method of common hazel propagation / Kushnarenko S.V., Aralbaeva M.M., Romadanova N.V. // No. 4998; Declared Oct. 30, 2019/0952.2., publ. 06.05.20. (in Russian) https://gosreestr.kazpatent.kz/Utilitymodel/Details?docNumber=320230
  2. Pat. No. 34902. Method for obtaining apple planting stocks recovered from viruses by chemotherapy / Romadanova N.V., Nurmanov M.M., Kushnarenko S.V. // No. 34902; application 01/31/2020/0056.1., publ. 02/26/21. (in Russian) https://gosreestr.kazpatent.kz/Invention/Details?docNumber=323672

 

Results for 2023: populations were described using geobotanical methods; descriptors from 35 to 49 indicators have been developed for plants, depending on the species. JPS coordinates were recorded at accessions collection sites.

In vitro initiation of S. schrenckianus and P. tenella shoots has begun. Seeds without stratification were germinated on Knop nutrient medium (viability — 20%). The seeds of all collected plants were placed into plastic containers for stratification for 8 weeks at 4°C. Seeds from 6 S. schrenckianus accessions were stratified in moist perlite for 8 weeks at 4°C. No endophytic contamination was detected in shoots of S. schrenckianus germinated on Knop medium after diagnosing test tube plants for the presence of endophytic contamination on specialized medium 523. The optimization of nutrient media for micropropagation of S. schrenckianus and the creation of its in vitro collection were being carried out.

The creation of a cryogenic bank of seeds and DNA at -196°C has begun. Seeds from 12 S. schrenckianus accessions were placed in liquid nitrogen for long-term storage. Total DNA was isolated from leaves of M. sieversi, S. persica and L. ulmifolia, and DNA quality was tested using electropherograms. As a result, 20 DNA accessions of S. persica, 16 accessions of M. sieversi, and 6 accessions of L. ulmifolia were placed in a cryogenic bank for long-term storage.

 

Publications (2023):

  • V. Romadanova, A.S. Zemtsova, A.M. Alexandrova, N.A. Altayeva, M.M. Aralbayeva, K.T. Abidkulova, N.V. Mikhailenko, A.B. Tolegen, S.V. Kushnarenko. Development of biotechnology for the ex-situ conservation of rare, endangered Rosaceae species , for the restoration of natural populations //November 27-29, 2023 — satellite events dedicated to the problems of preserving the biodiversity of rare, endangered plant species; growing fruit and berry crops, potatoes and perennial forage crops, including in the northern regions (in print).
  • S. Zemtsova, N.V. Romadanova, N.A. Altayeva, M.M. Aralbayeva, S.V. Kushnarenko. Conservation of rare and endangered plant species biodiversity of the Rosaceae: Spiraeanthus schrenkianus (Fisch. & C.A. Mey.) Maxim. and Sibiraea altaiensis (Laxm.) Schneid. //November 27-29, 2023 – satellite events dedicated to the problems of preserving the biodiversity of rare and endangered plant species; growing fruit and berry crops, potatoes and perennial forage crops, including in the northern regions (in print).
  • V. Romadanova, A.S. Zemtsova, A.M. Aleksandrova, N.A. Altayeva, M.M. Aralbayeva, K.T. Abidkulova, N.V. Mikhailenko, A.B. Tolegen, S.V. Kushnarenko. Development of biotechnology for ex situ conservation of rare, endangered Rosaceae species, for the restoration of natural populations //36th International Conference on Ecological Agriculture, Biodiversity &Waste Management (EABWM)/ Dec. 20-22, 2023 Bangkok (Thailand). P 16