Laboratory of Cell Engineering

Head of the laboratory

Dr. Kairat Uteulin,  Professor

Fax:  +7(727) 394 7562

Phone: 8 705 185 0224

E-mail: gen_uteulink@mail.ru

Author ID Scopus 6602865844

Researcher ID Web of Science L-3508-2018

ORCID ID 0000-0002-5459-0902

Genetic constructions of key genes to combat micropathogens and viruses penetration and infecting in barley have been created: Ac gene — chitinase that destroys the cell wall of microfungies; constriction for plant CRISPR / Cas9 genome editing and knockout of gene eukaryotic virus translation initiation factor (eIF4E) that involved in translation of many viruses caused crops diseases. A new simple and effective genotype-independent germ-line biotechnology method for genetic transformation of barley has been developed and patented. Genetic editing approaches for the barley eIF4E gene have been developed, including bombardment and Agrobacterium tumefaciens transformations. Genetic editing (modification) of the eIF4E gene is performing. Edited CRISPR / Cas9 barley lines with modification of the eIF4E gene resistant to many viral diseases will be created.

Approaches to improve the natural resistance of plants to biotic stresses and to create disease-resistant soybean lines by genetic engineering of the phenylpropanoid cycle have been developed. Genetic engineering techniques for increasing the biosynthesis of lignin — a natural anti-microbial compound, to improve the control of micro-pathogens that caused diseases have been established. Genetic constructions of the key genes of phenylpropanoid cycle have been elaborated and applied in the genetic transformation of soybean: transcription factor Cs/MYB4_sens; the main lignification genes — 35S/PAL5, C4H/F5H, 35S/CAD1, as well as gene antioxidant stress — FeSOD. The biotechnology of A. tumefaciens mediated germ-line genetic transformation of soybean plants was elaborated and  optimized for the introduction of key genes of lignin biosynthesis and resistance to biotic stresses and was used to obtain T₀ transgenes with the fficiency of beans forming 90% of transformed seeds number. Metabolic profiling of transgenic soybean plants showed a composition changing and an increasing of content of the most important metabolites of the phenylpropanoid cycle: flavonols, phenolic acids, genistin, saponins — antioxidants involved in protecting plants from biotic stresses.

So, the transition from Genomics to Metabolomics was carried out by improving the most important metabolic process — the phenylpropanoid cycle in soybean plants using genetic engineering techniques to improve resistance to biotic stresses — diseases caused by micropathogens.

New interspecific drought-resistant wheat hybrids with high grain quality for various purposes of the food industry were obtained.

Kok-saghyz breeding technologies have been developed.

Methods for the extraction of rubber from Kok-saghyz roots have been developed.

Kershanskaya O.I. The Patent application was accepted and the formal examination of the patent for the invention “New method of genetic transformation of barley” 2019.0184.1 from 11.03.2019 was successfully completed. Authors: Kershanskaya OI, Esenbaeva G.L., Mukiyanova G.S., Nelidova DS, Sadullaeva Z.N.

Lee T.E. et al. A method for identifying high-protein leguminous crops. The number of the utility model is 30716. Published 12/25/2015

Terletskaya N.V., Altaeva N.A., Zorbekova A.N. A way to create alloplasmatic drought-resistant wheat line. Utility model number: 107266. Published 12/21/2018

Terletskaya N.V. et al. A method for producing photosynthetic callus wheat culture. Utility model number: 1642. Published: 09/15/2016

Altaeva N.A., Khaylenko N.A. The method for determining hybrid wheat plants in interspecific crosses with the species T.kiharae. The number of the utility model: 1751. Posted 09/26/2016

Uteulin KR, et al. The method of obtaining the original seeds “TKS-1 ″ — prototype varieties of kok-sagyz (Taraxacum kok-saghyz). Innovation patent number: 31285. Published: 06/30/2016.

Uteulin K.R., Zhambakin K.J. et al. Certificate of the author of a selection achievement. Variety of Dandelion, kok-saghyz «Saryzhaz» No. 4395 (2018).

PhD doctoral student Gabit Bari (2019-2021), supervisor Uteulin K.R.«Obtaining highly productive kok-saghyz  (Taraxacum kok-saghyz L.) forms based on variability of morpho-physiological features».

PhD doctoral student . Erezhetova Ulzhan (2019-2021). supervisor Terletskaya N. “The effect of drought on the anatomical, morphological and photosynthetic parameters of various species and interspecific wheat hybrids in the agrocenosis”.

2 master’s theses were defended: Kuly Z.T., Maulenbay A.D. KazNU, Master of Technical Sciences, KazNU, 6M070100 — Biotechnology. June 2018. Supervisor Kershanskaya O.I.

Defence of the Bachelor’s of Sciences Diploma, KazNAU: Sadullaeva Z.N. 2019. Thesis Manager Candidate of Agricultural Sciences Yesenbayeva G.L.

Kershanskaya O.I.:

Real Member – Academician of Petri Prima Academy of Science and Arts (PANA), Russia, ID RAN 1227, from 22 June 2006.

Member of EBTNA (European Biotechnology Thematic Network Association).

Member of SPR (Russian Society of Plant Physiologists).

Fulbright Foreign Visitor CIES, USDS, University of Illinois at Urbana-Champaign, Illinois, USA. Senior Researcher. “Soybean stress resistance improvement via genetic engineering of phenylpropanoid cycle”. 2013-2015.

Fulbright Certificate of Completion. Chairman, J. William Fulbright Foreign Scholarship Board, Assistant Secretary of State for Educational and Cultural Affairs, Washington DC, USA.

Terletskaya N.V.:

received in 2018 the title of associate professor.